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. 1988 Jan;42(1):60–65.

A new approach in recognition of heterochromatic regions of human chromosomes by means of restriction endonucleases.

A Babu 1, A K Agarwal 1, R S Verma 1
PMCID: PMC1715309  PMID: 2827464

Abstract

The heteromorphisms of C-band regions of human chromosomes are evaluated by means of restriction endonucleases AluI, DdeI, MboI, and RsaI. Every chromosome exhibits heteromorphic markers of the C-band regions except chromosome 8. Each enzyme was found to be highly characteristic in its staining profile, a result that clearly suggests the diversity of heterochromatin. The inherent C-band-region heterochromatin variability that is revealed by these enzymes provides a valuable tool in identifying markers as compared with other previously described techniques.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babu A., Verma R. S., Patil S. R. AluI-resistant chromatin of chromosome 18: classification, frequencies and implications. Chromosoma. 1987;95(3):163–166. doi: 10.1007/BF00330345. [DOI] [PubMed] [Google Scholar]
  2. Bianchi M. S., Bianchi N. O., Pantelias G. E., Wolff S. The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes. Chromosoma. 1985;91(2):131–136. doi: 10.1007/BF00294057. [DOI] [PubMed] [Google Scholar]
  3. Bianchi N. O., Bianchi M. S., Cleaver J. E. The action of ultraviolet light on the patterns of banding induced by restriction endonucleases in human chromosomes. Chromosoma. 1984;90(2):133–138. doi: 10.1007/BF00292450. [DOI] [PubMed] [Google Scholar]
  4. Jacobs P. A. Human chromosome heteromorphisms (variants). Prog Med Genet. 1977;2:251–274. doi: 10.1016/0378-1119(77)90004-x. [DOI] [PubMed] [Google Scholar]
  5. Kaelbling M., Miller D. A., Miller O. J. Restriction enzyme banding of mouse metaphase chromosomes. Chromosoma. 1984;90(2):128–132. doi: 10.1007/BF00292449. [DOI] [PubMed] [Google Scholar]
  6. McKenzie W. H., Lubs H. A. Human Q and C chromosomal variations: distribution and incidence. Cytogenet Cell Genet. 1975;14(2):97–115. doi: 10.1159/000130330. [DOI] [PubMed] [Google Scholar]
  7. Mezzanotte R., Bianchi U., Vanni R., Ferrucci L. Chromatin organization and restriction endonuclease activity on human metaphase chromosomes. Cytogenet Cell Genet. 1983;36(3):562–566. doi: 10.1159/000131973. [DOI] [PubMed] [Google Scholar]
  8. Miller D. A., Choi Y. C., Miller O. J. Chromosome localization of highly repetitive human DNA's and amplified ribosomal DNA with restriction enzymes. Science. 1983 Jan 28;219(4583):395–397. doi: 10.1126/science.6294832. [DOI] [PubMed] [Google Scholar]
  9. Miller D. A., Gosden J. R., Hastie N. D., Evans H. J. Mechanism of endonuclease banding of chromosomes. Exp Cell Res. 1984 Nov;155(1):294–298. doi: 10.1016/0014-4827(84)90793-6. [DOI] [PubMed] [Google Scholar]
  10. Olson S. B., Magenis R. E., Lovrien E. W. Human chromosome variation: the discriminatory power of Q-band heteromorphism (variant) analysis in distinguishing between individuals, with specific application to cases of questionable paternity. Am J Hum Genet. 1986 Feb;38(2):235–252. [PMC free article] [PubMed] [Google Scholar]
  11. Phillips R. B. New C band markers of human chromosomes: C band position variants. J Med Genet. 1980 Oct;17(5):380–385. doi: 10.1136/jmg.17.5.380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Verma R. S., Dosik H. Human chromosomal heteromorphisms: nature and clinical significance. Int Rev Cytol. 1980;62:361–333. doi: 10.1016/s0074-7696(08)61903-8. [DOI] [PubMed] [Google Scholar]

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