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. 1988 Jan;42(1):143–159.

Identification of more than 500 RFLPs by screening random genomic clones

James W Schumm, Robert G Knowlton, Jeffrey C Braman, David F Barker, David Botstein, Gita Akots, Valerie A Brown, Thomas C Gravius, Cynthia Helms, Kathy Hsiao, Kenneth Rediker, Joan G Thurston, Helen Donis-Keller
PMCID: PMC1715337  PMID: 2892396

Abstract

As part of our genome-mapping effort, we undertook a large-scale screening study to identify RFLPs useful as genetic markers. Some 1,664 single-copy or repeat-containing phage clones from a Charon 4A genomic library were tested for polymorphism against a panel of DNAs, from five unrelated individuals, digested with eight restriction enzymes. Approximately 30% (515) of the clones revealed polymorphism by Southern hybridization; 67 loci detected had PIC values >.5. Restriction enzymes MspI, TaqI, and RsaI were most efficient in detecting polymorphism within the 1–20-kb-fragment size range resolved. With only one exception each of the clones detected polymorphism originating from a single locus.

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Selected References

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