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. 1988 Jul;32(7):1086–1089. doi: 10.1128/aac.32.7.1086

In vitro cleavable-complex assay to monitor antimicrobial potency of quinolones.

L Walton 1, L P Elwell 1
PMCID: PMC172350  PMID: 2847641

Abstract

Seven quinolones were evaluated to determine whether their ability to generate the DNA gyrase-mediated cleavable complex correlated with their ability to inhibit the catalytic activity of purified DNA gyrase and inhibit the growth of Escherichia coli. The rank order of potency of these drugs in the cleavable-complex assay was essentially the same as in the DNA supercoiling-inhibition assay. It required 2- to 10-fold-lower drug concentrations to generate the cleavable complex than to inhibit E. coli DNA gyrase. With the newer fluoroquinolones, a 25- to 100-fold-greater concentration was required for DNA gyrase inhibition than for cell growth inhibition, suggesting a more subtle interaction between these inhibitors and DNA gyrase than mere enzyme inhibition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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