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. 2002 Mar;57(3):263–266. doi: 10.1136/thorax.57.3.263

Serum sialyl Lewis X-i antigen in lung adenocarcinoma and idiopathic pulmonary fibrosis

H Satoh 1, H Ishikawa 1, Y Yamashita 1, M Ohtsuka 1, K Sekizawa 1
PMCID: PMC1746275  PMID: 11867833

Abstract

Background: Sialyl Lewis X-i antigen has been used as a diagnostic tool for lung adenocarcinoma. However, serum levels of the antigen are also raised in some patients with idiopathic pulmonary fibrosis (IPF) without coexistent malignancy. Expression of the antigen in serum samples of patients with lung adenocarcinoma was evaluated and compared with that of patients with IPF by Western blotting in order to establish a specific laboratory test to differentiate lung adenocarcinoma from IPF.

Methods: The pattern of antigen expression in serum samples from 23 patients with either lung adenocarcinoma or non-malignant lung disease in whom serum levels of sialyl Lewis X-i antigen (>50 U/ml) were significantly increased was studied by Western blotting.

Results: Thirteen of the 14 serum samples from patients with lung adenocarcinoma had a molecular weight band at 120 or 130 kD, while five of the six patients with IPF had two or three bands at <97.4 kD. The pattern of antigen expression was apparently different between the two diseases. The sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio of this test in 20 patients with lung adenocarcinoma and IPF were 92.9%, 83.3%, 5.57, and 0.09, respectively.

Conclusions: Western blotting analysis of serum samples from patients with raised levels of sialyl Lewis X-i antigen provides some clinical information for a differential diagnosis between lung adenocarcinoma and IPF.

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Selected References

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  1. Abbona G. C., Papotti M., Gugliotta P., Pecchio F., Rapellino M. Immunohistochemical detection of carcinoembryonic antigen (CEA) in non-neoplastic lung disease. Int J Biol Markers. 1993 Oct-Dec;8(4):240–243. doi: 10.1177/172460089300800407. [DOI] [PubMed] [Google Scholar]
  2. Bast R. C., Jr, Feeney M., Lazarus H., Nadler L. M., Colvin R. B., Knapp R. C. Reactivity of a monoclonal antibody with human ovarian carcinoma. J Clin Invest. 1981 Nov;68(5):1331–1337. doi: 10.1172/JCI110380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beckett M. L., Wright G. L., Jr Characterization of a prostate carcinoma mucin-like antigen (PMA). Int J Cancer. 1995 Sep 15;62(6):703–710. doi: 10.1002/ijc.2910620610. [DOI] [PubMed] [Google Scholar]
  4. Bjoraker J. A., Ryu J. H., Edwin M. K., Myers J. L., Tazelaar H. D., Schroeder D. R., Offord K. P. Prognostic significance of histopathologic subsets in idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 1998 Jan;157(1):199–203. doi: 10.1164/ajrccm.157.1.9704130. [DOI] [PubMed] [Google Scholar]
  5. Brugiere O., Milleron B., Antoine M., Carette M. F., Philippe C., Mayaud C. Diffuse panbronchiolitis in an Asian immigrant. Thorax. 1996 Oct;51(10):1065–1067. doi: 10.1136/thx.51.10.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crystal R. G., Fulmer J. D., Roberts W. C., Moss M. L., Line B. R., Reynolds H. Y. Idiopathic pulmonary fibrosis. Clinical, histologic, radiographic, physiologic, scintigraphic, cytologic, and biochemical aspects. Ann Intern Med. 1976 Dec;85(6):769–788. doi: 10.7326/0003-4819-85-6-769. [DOI] [PubMed] [Google Scholar]
  7. Fitzgerald J. E., King T. E., Jr, Lynch D. A., Tuder R. M., Schwarz M. I. Diffuse panbronchiolitis in the United States. Am J Respir Crit Care Med. 1996 Aug;154(2 Pt 1):497–503. doi: 10.1164/ajrccm.154.2.8756828. [DOI] [PubMed] [Google Scholar]
  8. Gay S. E., Kazerooni E. A., Toews G. B., Lynch J. P., 3rd, Gross B. H., Cascade P. N., Spizarny D. L., Flint A., Schork M. A., Whyte R. I. Idiopathic pulmonary fibrosis: predicting response to therapy and survival. Am J Respir Crit Care Med. 1998 Apr;157(4 Pt 1):1063–1072. doi: 10.1164/ajrccm.157.4.9703022. [DOI] [PubMed] [Google Scholar]
  9. Gold P., Freedman S. O. Specific carcinoembryonic antigens of the human digestive system. J Exp Med. 1965 Sep 1;122(3):467–481. doi: 10.1084/jem.122.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirasawa Y., Kohno N., Yokoyama A., Kondo K., Hiwada K., Miyake M. Natural autoantibody to MUC1 is a prognostic indicator for non-small cell lung cancer. Am J Respir Crit Care Med. 2000 Feb;161(2 Pt 1):589–594. doi: 10.1164/ajrccm.161.2.9905028. [DOI] [PubMed] [Google Scholar]
  11. Itzkowitz S. H., Yuan M., Fukushi Y., Lee H., Shi Z. R., Zurawski V., Jr, Hakomori S., Kim Y. S. Immunohistochemical comparison of Lea, monosialosyl Lea (CA 19-9), and disialosyl Lea antigens in human colorectal and pancreatic tissues. Cancer Res. 1988 Jul 1;48(13):3834–3842. [PubMed] [Google Scholar]
  12. Itzkowitz S. H., Yuan M., Montgomery C. K., Kjeldsen T., Takahashi H. K., Bigbee W. L., Kim Y. S. Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer. Cancer Res. 1989 Jan 1;49(1):197–204. [PubMed] [Google Scholar]
  13. Kannagi R., Fukushi Y., Tachikawa T., Noda A., Shin S., Shigeta K., Hiraiwa N., Fukuda Y., Inamoto T., Hakomori S. Quantitative and qualitative characterization of human cancer-associated serum glycoprotein antigens expressing fucosyl or sialyl-fucosyl type 2 chain polylactosamine. Cancer Res. 1986 May;46(5):2619–2626. [PubMed] [Google Scholar]
  14. Kondo K., Tsuzuki H., Sasa M., Sumitomo M., Uyama T., Monden Y. A dose-response relationship between the frequency of p53 mutations and tobacco consumption in lung cancer patients. J Surg Oncol. 1996 Jan;61(1):20–26. doi: 10.1002/(SICI)1096-9098(199601)61:1<20::AID-JSO6>3.0.CO;2-U. [DOI] [PubMed] [Google Scholar]
  15. Ligtenberg M. J., Vos H. L., Gennissen A. M., Hilkens J. Episialin, a carcinoma-associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini. J Biol Chem. 1990 Apr 5;265(10):5573–5578. [PubMed] [Google Scholar]
  16. Matsushita Y., Cleary K. R., Ota D. M., Hoff S. D., Irimura T. Sialyl-dimeric Lewis-X antigen expressed on mucin-like glycoproteins in colorectal cancer metastases. Lab Invest. 1990 Dec;63(6):780–791. [PubMed] [Google Scholar]
  17. Matsushita Y., Nakamori S., Seftor E. A., Hendrix M. J., Irimura T. Human colon carcinoma cells with increased invasive capacity obtained by selection for sialyl-dimeric LeX antigen. Exp Cell Res. 1991 Sep;196(1):20–25. doi: 10.1016/0014-4827(91)90451-y. [DOI] [PubMed] [Google Scholar]
  18. Michaelson J. E., Aguayo S. M., Roman J. Idiopathic pulmonary fibrosis: a practical approach for diagnosis and management. Chest. 2000 Sep;118(3):788–794. doi: 10.1378/chest.118.3.788. [DOI] [PubMed] [Google Scholar]
  19. Miyake M., Zenita K., Tanaka O., Okada Y., Kannagi R. Stage-specific expression of SSEA-1-related antigens in the developing lung of human embryos and its relation to the distribution of these antigens in lung cancers. Cancer Res. 1988 Dec 15;48(24 Pt 1):7150–7158. [PubMed] [Google Scholar]
  20. Mukae H., Hirota M., Kohno S., Komori K., Fukushima K., Hiratani K., Kadota J., Hara K. Elevation of tumor-associated carbohydrate antigens in patients with diffuse panbronchiolitis. Am Rev Respir Dis. 1993 Sep;148(3):744–751. doi: 10.1164/ajrccm/148.3.744. [DOI] [PubMed] [Google Scholar]
  21. Sasaki M., Morita T., Hoso M., Nakanuma Y., Tanimura H. Carcinoembryonic antigen and blood group-related carbohydrate antigens in glycoproteins in human bile in hepatolithiasis. Japanese Study Group of Hepatolithiasis. Hepatology. 1996 Feb;23(2):258–263. doi: 10.1002/hep.510230210. [DOI] [PubMed] [Google Scholar]
  22. Satoh H., Ishikawa H., Kamma H., Yamashita Y. T., Takahashi H., Ohtsuka M., Hasegawa S. Serum sialyl lewis X-i antigen levels in non-small cell lung cancer: correlation with distant metastasis and survival. Clin Cancer Res. 1997 Apr;3(4):495–499. [PubMed] [Google Scholar]
  23. Satoh H., Kamma H., Ogata T., Yano H., Ohtsuka M., Hasegawa S. Clinical significance of serum levels of a carbohydrate antigen, sialyl SSEA-1, in patients with fibrosing lung disease. Am Rev Respir Dis. 1991 Nov;144(5):1177–1181. doi: 10.1164/ajrccm/144.5.1177. [DOI] [PubMed] [Google Scholar]
  24. Satoh H., Yano H., Naitoh T., Takahashi N., Suyama T., Murayama J., Kameyama M., Fukuda K., Satoh T., Ohtsuka M. [Determination of various tumor markers, with special reference to sialyl SSEA-1 antigen in lung cancer]. Gan To Kagaku Ryoho. 1988 Oct;15(10):2917–2922. [PubMed] [Google Scholar]
  25. Sikut R., Zhang K., Baeckström D., Hansson G. C. Distinct sub-populations of carcinoma-associated MUC1 mucins as detected by the monoclonal antibody 9H8 and antibodies against the sialyl-Lewis a and sialyl-Lewis x epitopes in the circulation of breast-cancer patients. Int J Cancer. 1996 May 29;66(5):617–623. doi: 10.1002/(SICI)1097-0215(19960529)66:5<617::AID-IJC6>3.0.CO;2-W. [DOI] [PubMed] [Google Scholar]
  26. Singhal A. K., Orntoft T. F., Nudelman E., Nance S., Schibig L., Stroud M. R., Clausen H., Hakomori S. Profiles of Lewisx-containing glycoproteins and glycolipids in sera of patients with adenocarcinoma. Cancer Res. 1990 Mar 1;50(5):1375–1380. [PubMed] [Google Scholar]
  27. Takahashi H., Nukiwa T., Matsuoka R., Danbara T., Natori H., Arai T., Kira S. Carcinoembryonic antigen in bronchoalveolar lavage fluid in patients with idiopathic pulmonary fibrosis. Jpn J Med. 1985 Aug;24(3):236–243. doi: 10.2169/internalmedicine1962.24.236. [DOI] [PubMed] [Google Scholar]
  28. Takayama S., Kataoka N., Usui Y., Inase N., Natori Y., Nakayama M., Miura H., Kimula Y. [CA 19-9 in patients with benign pulmonary diseases]. Nihon Kyobu Shikkan Gakkai Zasshi. 1990 Oct;28(10):1326–1331. [PubMed] [Google Scholar]
  29. Turner-Warwick M., Lebowitz M., Burrows B., Johnson A. Cryptogenic fibrosing alveolitis and lung cancer. Thorax. 1980 Jul;35(7):496–499. doi: 10.1136/thx.35.7.496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zenita K., Kirihata Y., Kitahara A., Shigeta K., Higuchi K., Hirashima K., Murachi T., Miyake M., Takeda T., Kannagi R. Fucosylated type-2 chain polylactosamine antigens in human lung cancer. Int J Cancer. 1988 Mar 15;41(3):344–349. doi: 10.1002/ijc.2910410304. [DOI] [PubMed] [Google Scholar]

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