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. 1987 Feb;31(2):306–311. doi: 10.1128/aac.31.2.306

Organization of two sulfonamide resistance genes on plasmids of gram-negative bacteria.

G Swedberg
PMCID: PMC174711  PMID: 3032095

Abstract

The organization of two widely distributed sulfonamide resistance genes has been studied. The type I gene was linked to other resistance genes, like streptomycin resistance in R100 and trimethoprim resistance in R388 and other recently isolated plasmids from Sri Lanka. In R388, the sulfonamide resistance gene was transcribed from a promoter of its own, but in all other studied plasmids the linked genes were transcribed from a common promoter. This was especially established with a clone derived from plasmid R6-5, in which transposon mutagenesis showed that expression of sulfonamide resistance was completely dependent on the linked streptomycin resistance gene. The type II sulfonamide resistance gene was independently transcribed and found on two kinds of small resistance plasmids and also on large plasmids isolated from clinical material.

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Selected References

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  1. Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  3. CLOWES R. C., ROWLEY D. Some observations on linkage effects in genetic recombination in Escherichia coli K-12. J Gen Microbiol. 1954 Oct;11(2):250–260. doi: 10.1099/00221287-11-2-250. [DOI] [PubMed] [Google Scholar]
  4. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  5. Guerry P., van Embden J., Falkow S. Molecular nature of two nonconjugative plasmids carrying drug resistance genes. J Bacteriol. 1974 Feb;117(2):619–630. doi: 10.1128/jb.117.2.619-630.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heffron F., Rubens C., Falkow S. Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3623–3627. doi: 10.1073/pnas.72.9.3623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Korfmann G., Lüdtke W., van Treeck U., Wiedemann B. Dissemination of streptomycin and sulfonamide resistance by plasmid pBP1 in Escherichia coli. Eur J Clin Microbiol. 1983 Oct;2(5):463–468. doi: 10.1007/BF02013905. [DOI] [PubMed] [Google Scholar]
  8. Kratz J., Schmidt F., Wiedemann B. Characterization of Tn2411 and Tn2410, two transposons derived from R-plasmid R1767 and related to Tn2603 and Tn21. J Bacteriol. 1983 Sep;155(3):1333–1342. doi: 10.1128/jb.155.3.1333-1342.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  11. Stüber D., Bujard H. Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci U S A. 1981 Jan;78(1):167–171. doi: 10.1073/pnas.78.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Swedberg G., Sköld O. Characterization of different plasmid-borne dihydropteroate synthases mediating bacterial resistance to sulfonamides. J Bacteriol. 1980 Apr;142(1):1–7. doi: 10.1128/jb.142.1.1-7.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Swedberg G., Sköld O. Plasmid-borne sulfonamide resistance determinants studied by restriction enzyme analysis. J Bacteriol. 1983 Mar;153(3):1228–1237. doi: 10.1128/jb.153.3.1228-1237.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Timmis K. N., Cabello F., Cohen S. N. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol Gen Genet. 1978 Jun 14;162(2):121–137. doi: 10.1007/BF00267869. [DOI] [PubMed] [Google Scholar]
  15. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  16. Ward J. M., Grinsted J. Physical and genetic analysis of the Inc-W group plasmids R388, Sa, and R7K. Plasmid. 1982 May;7(3):239–250. doi: 10.1016/0147-619x(82)90005-1. [DOI] [PubMed] [Google Scholar]
  17. Wise E. M., Jr, Abou-Donia M. M. Sulfonamide resistance mechanism in Escherichia coli: R plasmids can determine sulfonamide-resistant dihydropteroate synthases. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2621–2625. doi: 10.1073/pnas.72.7.2621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. van Treeck U., Schmidt F., Wiedemann B. Molecular nature of a streptomycin and sulfonamide resistance plasmid (pBP1) prevalent in clinical Escherichia coli strains and integration of an ampicillin resistance transposon (TnA). Antimicrob Agents Chemother. 1981 Mar;19(3):371–380. doi: 10.1128/aac.19.3.371. [DOI] [PMC free article] [PubMed] [Google Scholar]

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