Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1987 Apr;31(4):660–662. doi: 10.1128/aac.31.4.660

Effect of phagocyte membrane stimulation on antibiotic uptake and intracellular bactericidal activity.

T H Steinberg, W L Hand
PMCID: PMC174803  PMID: 3606071

Abstract

Phagocytosis stimulated a substantial increase in clindamycin uptake and a smaller increase in rifampin accumulation by polymorphonuclear leukocytes. The effect of this increased antibiotic uptake on intraphagocytic bactericidal activity was evaluated. Although zymosan stimulated antibiotic uptake by polymorphonuclear leukocytes, neither zymosan nor formyl-methionyl-leucyl-phenylalanine enhanced the ability of clindamycin or rifampin to kill ingested staphylococci. Properties other than antibiotic uptake are important in determining intraphagocytic bactericidal activity.

Full text

PDF
660

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babior B. M. The respiratory burst of phagocytes. J Clin Invest. 1984 Mar;73(3):599–601. doi: 10.1172/JCI111249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borregaard N., Herlin T. Energy metabolism of human neutrophils during phagocytosis. J Clin Invest. 1982 Sep;70(3):550–557. doi: 10.1172/JCI110647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bøyum A. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol. 1976 Jun;Suppl 5:9–15. [PubMed] [Google Scholar]
  4. COHN Z. A., HIRSCH J. G. The influence of phagocytosis on the intracellular distribution of granule-associated components of polymorphonuclear leucocytes. J Exp Med. 1960 Dec 1;112:1015–1022. doi: 10.1084/jem.112.6.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crapo J. D., McCord J. M., Fridovich I. Preparation and assay of superoxide dismutases. Methods Enzymol. 1978;53:382–393. doi: 10.1016/s0076-6879(78)53044-9. [DOI] [PubMed] [Google Scholar]
  6. Hand W. L., Corwin R. W., Steinberg T. H., Grossman G. D. Uptake of antibiotics by human alveolar macrophages. Am Rev Respir Dis. 1984 Jun;129(6):933–937. doi: 10.1164/arrd.1984.129.6.933. [DOI] [PubMed] [Google Scholar]
  7. Hand W. L., King-Thompson N. L. Effect of erythrocyte ingestion on macrophage antibacterial function. Infect Immun. 1983 Jun;40(3):917–923. doi: 10.1128/iai.40.3.917-923.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hand W. L., King-Thompson N. L., Johnson J. D. Influence of bacterial-antibiotic interactions on subsequent antimicrobial activity of alveolar macrophages. J Infect Dis. 1984 Feb;149(2):271–276. doi: 10.1093/infdis/149.2.271. [DOI] [PubMed] [Google Scholar]
  9. Hand W. L., King-Thompson N. L. Membrane transport of clindamycin in alveolar macrophages. Antimicrob Agents Chemother. 1982 Feb;21(2):241–247. doi: 10.1128/aac.21.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Horwitz M. A. Phagocytosis of microorganisms. Rev Infect Dis. 1982 Jan-Feb;4(1):104–123. doi: 10.1093/clinids/4.1.104. [DOI] [PubMed] [Google Scholar]
  11. Jacobs R. F., Wilson C. B., Laxton J. G., Haas J. E., Smith A. L. Cellular uptake and intracellular activity of antibiotics against Haemophilus influenzae type b. J Infect Dis. 1982 Feb;145(2):152–159. doi: 10.1093/infdis/145.2.152. [DOI] [PubMed] [Google Scholar]
  12. Johnson J. D., Hand W. L., Francis J. B., King-Thompson N., Corwin R. W. Antibiotic uptake by alveolar macrophages. J Lab Clin Med. 1980 Mar;95(3):429–439. [PubMed] [Google Scholar]
  13. Lane T. A., Lamkin G. C., Windle B. E. Phagocytosis-induced modulation of human neutrophil chemotaxis receptors. Blood. 1981 Aug;58(2):228–236. [PubMed] [Google Scholar]
  14. Mandell G. L. Interaction of intraleukocytic bacteria and antibiotics. J Clin Invest. 1973 Jul;52(7):1673–1679. doi: 10.1172/JCI107348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mandell G. L., Vest T. K. Killing of intraleukocytic Staphylococcus aureus by rifampin: in-vitro and in-vivo studies. J Infect Dis. 1972 May;125(5):486–490. doi: 10.1093/infdis/125.5.486. [DOI] [PubMed] [Google Scholar]
  16. Prokesch R. C., Hand W. L. Antibiotic entry into human polymorphonuclear leukocytes. Antimicrob Agents Chemother. 1982 Mar;21(3):373–380. doi: 10.1128/aac.21.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Root R. K., Metcalf J., Oshino N., Chance B. H2O2 release from human granulocytes during phagocytosis. I. Documentation, quantitation, and some regulating factors. J Clin Invest. 1975 May;55(5):945–955. doi: 10.1172/JCI108024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Steinberg T. H., Hand W. L. Effects of phagocytosis on antibiotic and nucleoside uptake by human polymorphonuclear leukocytes. J Infect Dis. 1984 Mar;149(3):397–403. doi: 10.1093/infdis/149.3.397. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES