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. 1988 Nov;32(11):1680–1683. doi: 10.1128/aac.32.11.1680

Sensitization by heat treatment of Escherichia coli K-12 cells to hydrophobic antibacterial compounds.

T Tsuchido 1, M Takano 1
PMCID: PMC175951  PMID: 3075437

Abstract

The sensitivities of intact and heat-injured cells of Escherichia coli K-12 to several antibacterial compounds were measured by the prolongation of growth delay. Cells exposed to sublethal heat became more sensitive to various hydrophobic compounds, such as medium-chain fatty acids, alkyl esters of p-hydroxybenzoic acid, and some kinds of antibiotics or dyes, than unheated cells; but there was a smaller or no increase in sensitivity to short-chain fatty acids, chloramphenicol, and vancomycin. The destruction by heat of a permeability barrier of the outer membrane may have sensitized the cells to hydrophobic compounds. The sensitization was much lower for a strain defective in lipopolysaccharide, which is important as a barrier against hydrophobic compounds.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Freese E., Sheu C. W., Galliers E. Function of lipophilic acids as antimicrobial food additives. Nature. 1973 Feb 2;241(5388):321–325. doi: 10.1038/241321a0. [DOI] [PubMed] [Google Scholar]
  2. Hancock R. E. Alterations in outer membrane permeability. Annu Rev Microbiol. 1984;38:237–264. doi: 10.1146/annurev.mi.38.100184.001321. [DOI] [PubMed] [Google Scholar]
  3. Hansch C., Clayton J. M. Lipophilic character and biological activity of drugs. II. The parabolic case. J Pharm Sci. 1973 Jan;62(1):1–21. doi: 10.1002/jps.2600620102. [DOI] [PubMed] [Google Scholar]
  4. Hitchener B. J., Egan A. F. Outer-membrane damage in sublethally heated Escherichia coli K-12. Can J Microbiol. 1977 Mar;23(3):311–318. doi: 10.1139/m77-046. [DOI] [PubMed] [Google Scholar]
  5. Katsui N., Tsuchido T., Hiramatsu R., Fujikawa S., Takano M., Shibasaki I. Heat-induced blebbing and vesiculation of the outer membrane of Escherichia coli. J Bacteriol. 1982 Sep;151(3):1523–1531. doi: 10.1128/jb.151.3.1523-1531.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Leive L. Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate. J Biol Chem. 1968 May 10;243(9):2373–2380. [PubMed] [Google Scholar]
  7. Leive L. The barrier function of the gram-negative envelope. Ann N Y Acad Sci. 1974 May 10;235(0):109–129. doi: 10.1111/j.1749-6632.1974.tb43261.x. [DOI] [PubMed] [Google Scholar]
  8. Nikaido H. Outer membrane of Salmonella typhimurium. Transmembrane diffusion of some hydrophobic substances. Biochim Biophys Acta. 1976 Apr 16;433(1):118–132. doi: 10.1016/0005-2736(76)90182-6. [DOI] [PubMed] [Google Scholar]
  9. Nikaido H., Vaara M. Molecular basis of bacterial outer membrane permeability. Microbiol Rev. 1985 Mar;49(1):1–32. doi: 10.1128/mr.49.1.1-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sheu C. W., Freese E. Lipopolysaccharide layer protection of gram-negative bacteria against inhibition by long-chain fatty acids. J Bacteriol. 1973 Sep;115(3):869–875. doi: 10.1128/jb.115.3.869-875.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tamaki S., Sato T., Matsuhashi M. Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12. J Bacteriol. 1971 Mar;105(3):968–975. doi: 10.1128/jb.105.3.968-975.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tsuchido T., Ahn Y. H., Takano M. Lysis of Bacillus subtilis Cells by Glycerol and Sucrose Esters of Fatty Acids. Appl Environ Microbiol. 1987 Mar;53(3):505–508. doi: 10.1128/aem.53.3.505-508.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tsuchido T., Hiraoka T., Takano M., Shibasaki I. Involvement of autolysin in cellular lysis of Bacillus subtilis induced by short- and medium-chain fatty acids. J Bacteriol. 1985 Apr;162(1):42–46. doi: 10.1128/jb.162.1.42-46.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tsuchido T., Katsui N., Takeuchi A., Takano M., Shibasaki I. Destruction of the outer membrane permeability barrier of Escherichia coli by heat treatment. Appl Environ Microbiol. 1985 Aug;50(2):298–303. doi: 10.1128/aem.50.2.298-303.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zimmermann W., Rosselet A. Function of the outer membrane of Escherichia coli as a permeability barrier to beta-lactam antibiotics. Antimicrob Agents Chemother. 1977 Sep;12(3):368–372. doi: 10.1128/aac.12.3.368. [DOI] [PMC free article] [PubMed] [Google Scholar]

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