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. 2000 Sep;84(3):245–250. doi: 10.1136/heart.84.3.245

Myocarditis mimicking acute myocardial infarction: role of endomyocardial biopsy in the differential diagnosis

A Angelini 1, V Calzolari 1, F Calabrese 1, G Boffa 1, F Maddalena 1, R Chioin 1, G Thiene 1
PMCID: PMC1760950  PMID: 10956283

Abstract

OBJECTIVE—To test the hypothesis, using endomyocardial biopsies, that unexplained cases of apparent acute myocardial infarction were caused by myocarditis.
MATERIAL—Between 1992 and 1998, 12 patients were admitted to the coronary care unit with severe chest pain, ST segment elevation, increased serum creatine kinase and MB isoenzyme, and with wall motion abnormalities on echocardiogram highly suggestive of acute myocardial infarction. These patients were further investigated by endomyocardial biopsy, as their coronary angiograms were normal. A diagnosis of myocarditis was made according to the Dallas criteria. A panel of antibodies was used for immunohistochemical characterisation of inflammatory cell infiltrate. Polymerase chain reaction (PCR) was used to detect viral genomes in seven cases.
RESULTS—Haematoxylin and eosin staining of the endomyocardial biopsy showed active myocarditis in six patients and borderline myocarditis in one. Immunohistochemistry was positive for inflammatory cell infiltrates in 11 patients, including all the seven who were positive on haematoxylin and eosin staining according to the Dallas criteria. Only one patient had no evidence of inflammation. PCR was positive in two patients, both for Epstein-Barr virus. Follow up showed complete resolution of echocardiographic abnormalities in all patients except one.
CONCLUSIONS—Myocarditis can mimic acute myocardial infarction in patients with angiographically normal coronary arteries, leading to errors of treatment. In patients with apparent myocardial infarction and a normal coronary angiogram, endomyocardial biopsy may help in the diagnosis of myocarditis. The sensitivity of endomyocardial biopsy was enhanced by using immunohistochemical and molecular biological techniques.


Keywords: acute myocardial infarction; endomyocardial biopsy; myocarditis

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Figure 1  .

Figure 1  

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ECG traces on admission for patient 1 (A) and patient 6 (B). Note the ST segment elevation.

Figure 2  .

Figure 2  

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Time course of elevation of cardiac enzymes (creatine kinase (CK) and MB isoenzyme fraction). Values are expressed as means, error bars = SEM.

Figure 3  .

Figure 3  

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Patient 9: active lymphocytic myocarditis. Haematoxylin and eosin stain. (A) Inflammatory infiltrate surrounding a few necrotic myocytes. Immunohistochemical staining for CD45 (leucocytes) (B), for CD43 (lymphocytes) (C), and for CD 68 (macrophages) (D) confirmed the presence of inflammatory cells within the myocardium.

Figure 4  .

Figure 4  

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Patient 5. Haematoxylin and eosin stain. (A) No evidence of myocardial inflammation. Immunohistochemical staining for CD45 (B) showed the presence of leucocytes in the myocardium. (C) Polymerase chain reaction (PCR) for Epstein-Barr virus (EBV) performed on endomyocardial biopsy. The products were detected by ethidium bromide staining of 3% agarose gel. Lane 1, molecular weight marker; lane 2, EBV positive control (269 bp amplimer); lane 3, patient 5; lane 4, negative control (PCR reactants minus nucleic acid).

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