Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1985 Nov;28(5):626–629. doi: 10.1128/aac.28.5.626

Cytotoxicity of rabbit macrophage peptides MCP-1 and MCP-2 for mouse tumor cells.

M J Sheu, W W Baldwin, K W Brunson
PMCID: PMC176345  PMID: 4091528

Abstract

The cytotoxicity of cationic peptides MCP-1 and MCP-2 isolated from rabbit alveolar macrophages was tested against two tumor cell lines of murine lymphosarcoma origin, RAW117-P and RAW117-H10, and a normal mouse connective tissue fibroblast strain, ATCC CCL1.RAW117-H10 is a highly malignant metastatic variant derived from the less malignant RAW117-P. Our results indicate that these peptides possess a cytotoxic activity against the tumor cells tested but not against the normal cells tested. At concentrations of 30 micrograms/ml, these peptides completely killed RAW117-H10 cells in suspension cultures, while inhibiting growth of RAW117-P for but a limited period of time, up to 48 h, after which growth resumed. RAW117-P cells were killed by concentrations of 40 micrograms/ml. These peptides showed little cytotoxicity for normal mouse connective tissue fibroblasts at concentrations of 40 micrograms/ml.

Full text

PDF
627

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams D. O., Johnson W. J., Fiorito E., Nathan C. F. Hydrogen peroxide and cytolytic factor can interact synergistically in effecting cytolysis of neoplastic targets. J Immunol. 1981 Nov;127(5):1973–1977. [PubMed] [Google Scholar]
  2. Adams D. O., Johnson W. J., Marino P. A. Mechanisms of target recognition and destruction in macrophage-mediated tumor cytotoxicity. Fed Proc. 1982 Apr;41(6):2212–2221. [PubMed] [Google Scholar]
  3. Brunson K. W., Nicolson G. L. Selection and biologic properties of malignant variants of a murine lymphosarcoma. J Natl Cancer Inst. 1978 Dec;61(6):1499–1503. [PubMed] [Google Scholar]
  4. Lehrer R. I., Ferrari L. G., Patterson-Delafield J., Sorrell T. Fungicidal activity of rabbit alveolar and peritoneal macrophages against Candida albicans. Infect Immun. 1980 Jun;28(3):1001–1008. doi: 10.1128/iai.28.3.1001-1008.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lehrer R. I., Ladra K. M., Hake R. B. Nonoxidative fungicidal mechanisms of mammalian granulocytes: demonstration of components with candidacidal activity in human, rabbit, and guinea pig leukocytes. Infect Immun. 1975 Jun;11(6):1226–1234. doi: 10.1128/iai.11.6.1226-1234.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lehrer R. I., Selsted M. E., Szklarek D., Fleischmann J. Antibacterial activity of microbicidal cationic proteins 1 and 2, natural peptide antibiotics of rabbit lung macrophages. Infect Immun. 1983 Oct;42(1):10–14. doi: 10.1128/iai.42.1.10-14.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lepoivre M., Tenu J. P., Lemaire G., Petit J. F. Antitumor activity and hydrogen peroxide release by macrophages elicited by trehalose diesters. J Immunol. 1982 Aug;129(2):860–866. [PubMed] [Google Scholar]
  8. Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. doi: 10.1016/0003-2697(80)90470-4. [DOI] [PubMed] [Google Scholar]
  9. Panke E. S., Zwilling B. S., Somers S. D., Campolito L. B., Packer B. J. The effects of lipopolysaccharide, BCG-immune T lymphocytes, and lymphokines on generations of tumoricidal pulmonary macrophages in Syrian hamster. Exp Lung Res. 1982 Feb;3(1):81–90. doi: 10.3109/01902148209115818. [DOI] [PubMed] [Google Scholar]
  10. Patterson-Delafield J., Martinez R. J., Lehrer R. I. Microbicidal cationic proteins in rabbit alveolar macrophages: a potential host defense mechanism. Infect Immun. 1980 Oct;30(1):180–192. doi: 10.1128/iai.30.1.180-192.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
  12. Selsted M. E., Brown D. M., DeLange R. J., Lehrer R. I. Primary structures of MCP-1 and MCP-2, natural peptide antibiotics of rabbit lung macrophages. J Biol Chem. 1983 Dec 10;258(23):14485–14489. [PubMed] [Google Scholar]
  13. Sone S., Moriguchi S., Shimizu E., Ogushi F., Tsubura E. In vitro generation of tumoricidal properties in human alveolar macrophages following interaction with endotoxin. Cancer Res. 1982 Jun;42(6):2227–2231. [PubMed] [Google Scholar]
  14. Weinberg J. B., Chapman H. A., Jr, Hibbs J. B., Jr Characterization of the effects of endotoxin on macrophage tumor cell killing. J Immunol. 1978 Jul;121(1):72–80. [PubMed] [Google Scholar]
  15. Zwilling B. S., Campolito L. B. Destruction of tumor cells by BCG-activated alqolar macrophages. J Immunol. 1977 Sep;119(3):838–841. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES