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. 1973 Nov;49(3):543–553. doi: 10.1111/j.1476-5381.1973.tb17265.x

The continuous bioassay of the release and disappearance of histamine in the circulation

S H Ferreira, K K Ng, J R Vane
PMCID: PMC1776484  PMID: 4777714

Abstract

1. The blood-bathed organ technique was used for the continuous assay of histamine in circulating blood.

2. Longitudinal strips of cat or kitten terminal ileum detect concentrations of histamine of 2-5 ng/ml; these are below the threshold at which hypotension is induced in dogs.

3. The cat or kitten terminal ileum also contract to bradykinin. However, the simultaneous use of a cat jejunum strip, which is selectively sensitive to bradykinin, will distinguish between histamine and bradykinin.

4. The utility of the method for detecting and quantitating circulating histamine was exemplified by measuring: (a) its half-life in the circulation, (b) its disappearance during a passage through the vascular beds of lungs, liver and lower limbs, and (c) its release into the circulation by compound 48/80.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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