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. 2003 Aug;12(4):221–227. doi: 10.1080/09629350310001599666

Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients.

Smaragdi Antonopoulou 1, Alexandros Tsoupras 1, George Baltas 1, Helen Kotsifaki 1, Zacharias Mantzavinos 1, Constantinos A Demopoulos 1
PMCID: PMC1781616  PMID: 14514473

Abstract

Periodontal diseases are localized chronic inflammatory conditions of the gingival and underlying bone and connective tissue. Platelet-activating factor (PAF), a potent inflammatory phospholipid mediator that has been previously detected in elevated levels in inflamed gingival tissues, in gingival crevicular fluid and in saliva, is implicated in periodontal disease. Our results from previous studies showed that the biologically active phospholipid detected in gingival crevicular fluid is a hydroxyl-PAF analogue. In this study, hydroxyl-PAF analogue was detected for the first time in human blood derived from patients with chronic periodontitis as well as from periodontally healthy volunteers. The hydroxyl-PAF analogue was purified by high-performance liquid chromatography, detected by biological assays and identified by electrospray analysis. In addition, the quantitative determination of PAF and hydroxyl-PAF analogue (expressed as PAF-like activity) showed a statistically significant increase in the ratio of hydroxyl-PAF analogue levels to PAF levels in periodontal patients, suggesting that this bioactive lipid may play a role in oral inflammation.

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Selected References

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  1. Antonopoulou S., Demopoulos C. A., Argyropoulos D., Baltas G., Kotsifaki H., Diamanti-Kipioti A. Identification of a new endogenous platelet-activating factor-like molecule in gingival crevicular fluid. Biochem J. 1998 Mar 1;330(Pt 2):791–794. doi: 10.1042/bj3300791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armitage G. C. Periodontal infections and cardiovascular disease--how strong is the association? Oral Dis. 2000 Nov;6(6):335–350. doi: 10.1111/j.1601-0825.2000.tb00126.x. [DOI] [PubMed] [Google Scholar]
  3. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  4. Baltas G., Kotsifaki H., Antonopoulou S., Kipioti A., Demopoulos C. A. Implication of PAF and acetylhydrolase (PAF-AH) activity in periodontal disease. Adv Exp Med Biol. 1996;416:135–141. doi: 10.1007/978-1-4899-0179-8_23. [DOI] [PubMed] [Google Scholar]
  5. Beck J. D., Pankow J., Tyroler H. A., Offenbacher S. Dental infections and atherosclerosis. Am Heart J. 1999 Nov;138(5 Pt 2):S528–S533. doi: 10.1016/s0002-8703(99)70293-0. [DOI] [PubMed] [Google Scholar]
  6. Beck J., Garcia R., Heiss G., Vokonas P. S., Offenbacher S. Periodontal disease and cardiovascular disease. J Periodontol. 1996 Oct;67(10 Suppl):1123–1137. doi: 10.1902/jop.1996.67.10s.1123. [DOI] [PubMed] [Google Scholar]
  7. Brochériou I., Stengel D., Mattsson-Hultén L., Stankova J., Rola-Pleszczynski M., Koskas F., Wiklund O., Le Charpentier Y., Ninio E. Expression of platelet-activating factor receptor in human carotid atherosclerotic plaques: relevance to progression of atherosclerosis. Circulation. 2000 Nov 21;102(21):2569–2575. doi: 10.1161/01.cir.102.21.2569. [DOI] [PubMed] [Google Scholar]
  8. Brügger B., Erben G., Sandhoff R., Wieland F. T., Lehmann W. D. Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2339–2344. doi: 10.1073/pnas.94.6.2339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Demopoulos C. A., Andrikopoulos N. K., Antonopoulou S. A simple and precise method for the routine determination of platelet-activating factor in blood and urine. Lipids. 1994 Apr;29(4):305–309. doi: 10.1007/BF02536336. [DOI] [PubMed] [Google Scholar]
  10. Demopoulos C. A., Pinckard R. N., Hanahan D. J. Platelet-activating factor. Evidence for 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine as the active component (a new class of lipid chemical mediators). J Biol Chem. 1979 Oct 10;254(19):9355–9358. [PubMed] [Google Scholar]
  11. Emingil G., Cinarcik S., Baylas H., Hüseyinov A. Levels of platelet-activating factor in gingival crevicular fluid and gingival tissue in specific periodontal diseases. J Periodontol. 2001 Aug;72(8):1032–1037. doi: 10.1902/jop.2001.72.8.1032. [DOI] [PubMed] [Google Scholar]
  12. Khaselev N., Murphy R. C. Electrospray ionization mass spectrometry of lysoglycerophosphocholine lipid subclasses. J Am Soc Mass Spectrom. 2000 Apr;11(4):283–291. doi: 10.1016/s1044-0305(99)00158-0. [DOI] [PubMed] [Google Scholar]
  13. Kinane D. F. Periodontal diseases' contributions to cardiovascular disease: an overview of potential mechanisms. Ann Periodontol. 1998 Jul;3(1):142–150. doi: 10.1902/annals.1998.3.1.142. [DOI] [PubMed] [Google Scholar]
  14. Kolltveit K. M., Eriksen H. M. Is the observed association between periodontitis and atherosclerosis causal? Eur J Oral Sci. 2001 Feb;109(1):2–7. doi: 10.1034/j.1600-0722.2001.109001002.x. [DOI] [PubMed] [Google Scholar]
  15. Lazanas M., Demopoulos C. A., Tournis S., Koussissis S., Labrakis-Lazanas K., Tsarouhas X. PAF of biological fluids in disease: IV. Levels in blood in allergic reactions induced by drugs. Arch Dermatol Res. 1988;280(2):124–126. doi: 10.1007/BF00417717. [DOI] [PubMed] [Google Scholar]
  16. Liapikos T. A., Antonopoulou S., Karabina S. P., Tsoukatos D. C., Demopoulos C. A., Tselepis A. D. Platelet-activating factor formation during oxidative modification of low-density lipoprotein when PAF-acetylhydrolase has been inactivated. Biochim Biophys Acta. 1994 Jun 2;1212(3):353–360. doi: 10.1016/0005-2760(94)90210-0. [DOI] [PubMed] [Google Scholar]
  17. McManus L. M., Marze B. T., Schiess A. V. Deficiency of salivary PAF in edentulous individuals. J Periodontal Res. 1990 Nov;25(6):347–351. doi: 10.1111/j.1600-0765.1990.tb00926.x. [DOI] [PubMed] [Google Scholar]
  18. McManus L. M., Pinckard R. N. PAF, a putative mediator of oral inflammation. Crit Rev Oral Biol Med. 2000;11(2):240–258. doi: 10.1177/10454411000110020701. [DOI] [PubMed] [Google Scholar]
  19. Montrucchio G., Alloatti G., Camussi G. Role of platelet-activating factor in cardiovascular pathophysiology. Physiol Rev. 2000 Oct;80(4):1669–1699. doi: 10.1152/physrev.2000.80.4.1669. [DOI] [PubMed] [Google Scholar]
  20. Page R. C. The role of inflammatory mediators in the pathogenesis of periodontal disease. J Periodontal Res. 1991 May;26(3 Pt 2):230–242. doi: 10.1111/j.1600-0765.1991.tb01649.x. [DOI] [PubMed] [Google Scholar]
  21. Ribaldi E., Guerra M., Mezzasoma A. M., Staffolani N., Goracci G., Gresele P. PAF levels in saliva are regulated by inflammatory cells. J Periodontal Res. 1998 May;33(4):237–241. doi: 10.1111/j.1600-0765.1998.tb02195.x. [DOI] [PubMed] [Google Scholar]
  22. Socransky S. S., Haffajee A. D. Evidence of bacterial etiology: a historical perspective. Periodontol 2000. 1994 Jun;5:7–25. doi: 10.1111/j.1600-0757.1994.tb00016.x. [DOI] [PubMed] [Google Scholar]
  23. Stafforini D. M., Prescott S. M., McIntyre T. M. Human plasma platelet-activating factor acetylhydrolase. Purification and properties. J Biol Chem. 1987 Mar 25;262(9):4223–4230. [PubMed] [Google Scholar]
  24. Tokumura A., Sumida T., Toujima M., Kogure K., Fukuzawa K. Platelet-activating factor (PAF)-like oxidized phospholipids: relevance to atherosclerosis. Biofactors. 2000;13(1-4):29–33. doi: 10.1002/biof.5520130106. [DOI] [PubMed] [Google Scholar]
  25. Triggiani M., Schleimer R. P., Warner J. A., Chilton F. H. Differential synthesis of 1-acyl-2-acetyl-sn-glycero-3-phosphocholine and platelet-activating factor by human inflammatory cells. J Immunol. 1991 Jul 15;147(2):660–666. [PubMed] [Google Scholar]

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