Skip to main content
NCBI home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1999;8(1):37–41. doi: 10.1080/09629359990702

Prevention of renal injury after induction of ozone tolerance in rats submitted to warm ischaemia.

E Barber 1, S Menéndez 1, O S León 1, M O Barber 1, N Merino 1, J L Calunga 1, E Cruz 1, V Bocci 1
PMCID: PMC1781776  PMID: 10704088

Abstract

On the basis that ozone (O3) can upregulate cellular antioxidant enzymes, a morphological, biochemical and functional renal study was performed in rats undergoing a prolonged treatment with O3 before renal ischaemia. Rats were divided into four groups: (1) control, a medial abdominal incision was performed to expose the kidneys; (2) ischaemia, in animals undergoing a bilateral renal ischaemia (30 min), with subsequent reperfusion (3 h); (3) O3 + ischaemia, as group 2, but with previous treatment with O3 (0.5 mg/kg per day given in 2.5 ml O2) via rectal administration for 15 treatments; (4) O2 + ischaemia, as group 3, but using oxygen (O2) alone. Biochemical parameters as fructosamine level, phospholipase A, and superoxide dismutases (SOD) activities, as well as renal plasma flow (RPF) and glomerular filtration rate (GFR), were measured by means of plasma clearance of p-amino-hippurate and inulin, respectively. In comparison with groups 1 and 3, the RPF and GFR were significantly decreased in groups 2 and 4. Interestingly, renal homogenates of the latter groups yielded significantly higher values of phospholipase A activity and fructosamine level in comparison with either the control (1) and the O3 (3) treated groups. Moreover renal SOD activity showed a significant increase in group 3 without significant differences among groups 1, 2 and 4. Morphological alterations of the kidney were present in 100%, 88% and 30% of the animals in groups 2, 4 and 3, respectively. It is proposed that the O3 protective effect can be ascribed to the substantial possibility of upregulating the antioxidant defence system capable of counteracting the damaging effect of ischaemia. These findings suggest that, whenever possible, ozone preconditioning may represent a prophylactic approach for minimizing renal damage before transplantation.

Full Text

The Full Text of this article is available as a PDF (133.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belzer F. O., Southard J. H. Principles of solid-organ preservation by cold storage. Transplantation. 1988 Apr;45(4):673–676. doi: 10.1097/00007890-198804000-00001. [DOI] [PubMed] [Google Scholar]
  2. Bocci V. Is ozone therapy therapeutic? Perspect Biol Med. 1998 Autumn;42(1):131–143. doi: 10.1353/pbm.1998.0056. [DOI] [PubMed] [Google Scholar]
  3. Bocci V. Ozone as a bioregulator. Pharmacology and toxicology of ozonetherapy today. J Biol Regul Homeost Agents. 1996 Apr-Sep;10(2-3):31–53. [PubMed] [Google Scholar]
  4. Coudray C., Boucher F., Pucheu S., De Leiris J., Favier A. Relationship between severity of ischemia and oxidant scavenger enzyme activities in the isolated rat heart. Int J Biochem Cell Biol. 1995 Jan;27(1):61–69. doi: 10.1016/1357-2725(94)00055-7. [DOI] [PubMed] [Google Scholar]
  5. Cross C. E., Halliwell B., Borish E. T., Pryor W. A., Ames B. N., Saul R. L., McCord J. M., Harman D. Oxygen radicals and human disease. Ann Intern Med. 1987 Oct;107(4):526–545. doi: 10.7326/0003-4819-107-4-526. [DOI] [PubMed] [Google Scholar]
  6. Franssen C., Defraigne J. O., Detry O., Pincemail J., Deby C., Lamy M. Antioxidant defense and free radical production in a rabbit model of kidney ischemia-reperfusion. Transplant Proc. 1995 Oct;27(5):2880–2883. [PubMed] [Google Scholar]
  7. Halliwell B. Antioxidants in human health and disease. Annu Rev Nutr. 1996;16:33–50. doi: 10.1146/annurev.nu.16.070196.000341. [DOI] [PubMed] [Google Scholar]
  8. Hernández F., Menéndez S., Wong R. Decrease of blood cholesterol and stimulation of antioxidative response in cardiopathy patients treated with endovenous ozone therapy. Free Radic Biol Med. 1995 Jul;19(1):115–119. doi: 10.1016/0891-5849(94)00201-t. [DOI] [PubMed] [Google Scholar]
  9. Hotter G., Leon O. S., Roselló-Catafau J., López-Boado M. A., Parellada P. P., Henriques R. D., Fernández-Cruz L., Gelpí E. Tissular prostanoid release, phospholipase A2 activity, and lipid peroxidation in pancreas transplantation. Transplantation. 1991 May;51(5):987–990. doi: 10.1097/00007890-199105000-00011. [DOI] [PubMed] [Google Scholar]
  10. Johnson R. N., Metcalf P. A., Baker J. R. Fructosamine: a new approach to the estimation of serum glycosylprotein. An index of diabetic control. Clin Chim Acta. 1983 Jan 7;127(1):87–95. doi: 10.1016/0009-8981(83)90078-5. [DOI] [PubMed] [Google Scholar]
  11. Knight J. A. Diseases related to oxygen-derived free radicals. Ann Clin Lab Sci. 1995 Mar-Apr;25(2):111–121. [PubMed] [Google Scholar]
  12. León O. S., Menéndez S., Merino N., Castillo R., Sam S., Pérez L., Cruz E., Bocci V. Ozone oxidative preconditioning: a protection against cellular damage by free radicals. Mediators Inflamm. 1998;7(4):289–294. doi: 10.1080/09629359890983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Loeper J., Goy J., Rozensztajn L., Bedu O., Moisson P. Lipid peroxidation and protective enzymes during myocardial infarction. Clin Chim Acta. 1991 Feb 15;196(2-3):119–125. doi: 10.1016/0009-8981(91)90064-j. [DOI] [PubMed] [Google Scholar]
  14. McCord J. M. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med. 1985 Jan 17;312(3):159–163. doi: 10.1056/NEJM198501173120305. [DOI] [PubMed] [Google Scholar]
  15. McCoy R. N., Hill K. E., Ayon M. A., Stein J. H., Burk R. F. Oxidant stress following renal ischemia: changes in the glutathione redox ratio. Kidney Int. 1988 Apr;33(4):812–817. doi: 10.1038/ki.1988.72. [DOI] [PubMed] [Google Scholar]
  16. Murry C. E., Jennings R. B., Reimer K. A. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation. 1986 Nov;74(5):1124–1136. doi: 10.1161/01.cir.74.5.1124. [DOI] [PubMed] [Google Scholar]
  17. Nayak M. S., Kita M., Marmor M. F. Protection of rabbit retina from ischemic injury by superoxide dismutase and catalase. Invest Ophthalmol Vis Sci. 1993 May;34(6):2018–2022. [PubMed] [Google Scholar]
  18. Paller M. S., Hoidal J. R., Ferris T. F. Oxygen free radicals in ischemic acute renal failure in the rat. J Clin Invest. 1984 Oct;74(4):1156–1164. doi: 10.1172/JCI111524. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Parks D. A., Bulkley G. B., Granger D. N. Role of oxygen free radicals in shock, ischemia, and organ preservation. Surgery. 1983 Sep;94(3):428–432. [PubMed] [Google Scholar]
  20. Pryor W. A. Mechanisms of radical formation from reactions of ozone with target molecules in the lung. Free Radic Biol Med. 1994 Nov;17(5):451–465. doi: 10.1016/0891-5849(94)90172-4. [DOI] [PubMed] [Google Scholar]
  21. Sharma Y. K., Davis K. R. The effects of ozone on antioxidant responses in plants. Free Radic Biol Med. 1997;23(3):480–488. doi: 10.1016/s0891-5849(97)00108-1. [DOI] [PubMed] [Google Scholar]
  22. Smith H. W., Finkelstein N., Aliminosa L., Crawford B., Graber M. THE RENAL CLEARANCES OF SUBSTITUTED HIPPURIC ACID DERIVATIVES AND OTHER AROMATIC ACIDS IN DOG AND MAN. J Clin Invest. 1945 May;24(3):388–404. doi: 10.1172/JCI101618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Southard J. H., Marsh D. C., McAnulty J. F., Belzer F. O. Oxygen-derived free radical damage in organ preservation: activity of superoxide dismutase and xanthine oxidase. Surgery. 1987 May;101(5):566–570. [PubMed] [Google Scholar]
  24. Spector T. Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein. Anal Biochem. 1978 May;86(1):142–146. doi: 10.1016/0003-2697(78)90327-5. [DOI] [PubMed] [Google Scholar]
  25. Thome J., Münch G., Müller R., Schinzel R., Kornhuber J., Blum-Degen D., Sitzmann L., Rösler M., Heidland A., Riederer P. Advanced glycation endproducts-associated parameters in the peripheral blood of patients with Alzheimer's disease. Life Sci. 1996;59(8):679–685. doi: 10.1016/0024-3205(96)00349-9. [DOI] [PubMed] [Google Scholar]
  26. Wang P., Chen H., Qin H., Sankarapandi S., Becher M. W., Wong P. C., Zweier J. L. Overexpression of human copper, zinc-superoxide dismutase (SOD1) prevents postischemic injury. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4556–4560. doi: 10.1073/pnas.95.8.4556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Weller B. L., Crapo J. D., Slot J., Posthuma G., Plopper C. G., Pinkerton K. E. Site- and cell-specific alteration of lung copper/zinc and manganese superoxide dismutases by chronic ozone exposure. Am J Respir Cell Mol Biol. 1997 Nov;17(5):552–560. doi: 10.1165/ajrcmb.17.5.2753. [DOI] [PubMed] [Google Scholar]
  28. Yoshioka T., Bills T., Moore-Jarrett T., Greene H. L., Burr I. M., Ichikawa I. Role of intrinsic antioxidant enzymes in renal oxidant injury. Kidney Int. 1990 Aug;38(2):282–288. doi: 10.1038/ki.1990.197. [DOI] [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES