Skip to main content
Archives of Disease in Childhood logoLink to Archives of Disease in Childhood
. 1989 Nov;64(11):1599–1603. doi: 10.1136/adc.64.11.1599

Pseudomonas aeruginosa antibodies in blood spots from patients with cystic fibrosis.

V Thanasekaraan 1, M S Wiseman 1, R J Rayner 1, E J Hiller 1, D J Shale 1
PMCID: PMC1792658  PMID: 2513779

Abstract

The formation of antibodies to Pseudomonas aeruginosa may be the earliest indicator of pulmonary infection in patients with cystic fibrosis. To enable easy sampling in babies and young children an enzyme linked immunosorbent assay (ELISA) based on a blood spot sample taken on to blotting paper was developed. A sample of approximately 20 microliters of blood was required. A high correlation and level of absolute agreement was shown between paired finger prick and venepuncture blood spots, and between blood spot, serum spot, and serum samples. Healthy controls and non-infected patients with cystic fibrosis had low titres of antibody compared with patients with intermittent and chronic infection. The latter groups had significantly greater antibody titres than normal controls. This assay permits serial measurement of antibodies to P aeruginosa in patients of all ages with cystic fibrosis and may provide a means of assessing the value of such measurements in the detection and management of early infection.

Full text

PDF
1602

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bland J. M., Altman D. G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986 Feb 8;1(8476):307–310. [PubMed] [Google Scholar]
  2. Brett M. M., Ghoneim A. T., Littlewood J. M., Losowsky M. S. Development of enzyme linked immunosorbent assay (ELISA) to detect antibodies to Pseudomonas aeruginosa cell surface antigens in sera of patients with cystic fibrosis. J Clin Pathol. 1986 Oct;39(10):1124–1129. doi: 10.1136/jcp.39.10.1124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brett M. M., Ghoneim A. T., Littlewood J. M. Serum IgG antibodies in patients with cystic fibrosis with early Pseudomonas aeruginosa infection. Arch Dis Child. 1987 Apr;62(4):357–361. doi: 10.1136/adc.62.4.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bruce M. C., Poncz L., Klinger J. D., Stern R. C., Tomashefski J. F., Jr, Dearborn D. G. Biochemical and pathologic evidence for proteolytic destruction of lung connective tissue in cystic fibrosis. Am Rev Respir Dis. 1985 Sep;132(3):529–535. doi: 10.1164/arrd.1985.132.3.529. [DOI] [PubMed] [Google Scholar]
  5. Cost K. M., West C. S., Brinson D., Polk H. C., Jr Measurement of human antibody activity against Escherichia coli and Pseudomonas aeruginosa using formalin treated whole organisms in an ELISA technique. J Immunoassay. 1985;6(1-2):23–43. doi: 10.1080/01971528508063019. [DOI] [PubMed] [Google Scholar]
  6. Dasgupta M. K., Zuberbuhler P., Abbi A., Harley F. L., Brown N. E., Lam K., Dossetor J. B., Costerton J. W. Combined evaluation of circulating immune complexes and antibodies to Pseudomonas aeruginosa as an immunologic profile in relation to pulmonary function in cystic fibrosis. J Clin Immunol. 1987 Jan;7(1):51–58. doi: 10.1007/BF00915425. [DOI] [PubMed] [Google Scholar]
  7. Dussault J. H., Parlow A., Letarte J., Guyda H., Laberge C. TSH measurements from blood spots on filter paper: a confirmatory screening test for neonatal hypothyroidism. J Pediatr. 1976 Oct;89(4):550–552. doi: 10.1016/s0022-3476(76)80384-8. [DOI] [PubMed] [Google Scholar]
  8. GUTHRIE R., SUSI A. A SIMPLE PHENYLALANINE METHOD FOR DETECTING PHENYLKETONURIA IN LARGE POPULATIONS OF NEWBORN INFANTS. Pediatrics. 1963 Sep;32:338–343. [PubMed] [Google Scholar]
  9. Guimarães M. C., Castilho E. A., Celeste B. J., Nakahara O. S., Netto V. A. Long-term storage of IgG and IgM on filter paper for use in parasitic disease seroepidemiology surveys. Bull Pan Am Health Organ. 1985;19(1):16–28. [PubMed] [Google Scholar]
  10. Høiby N., Döring G., Schiøtz P. O. The role of immune complexes in the pathogenesis of bacterial infections. Annu Rev Microbiol. 1986;40:29–53. doi: 10.1146/annurev.mi.40.100186.000333. [DOI] [PubMed] [Google Scholar]
  11. Irie M., Enomoto K., Naruse H. Measurement of thyroid-stimulating hormone in dried blood spot. Lancet. 1975 Dec 20;2(7947):1233–1234. doi: 10.1016/s0140-6736(75)92072-3. [DOI] [PubMed] [Google Scholar]
  12. Larsen P. R., Merker A., Parlow A. F. Immunoassay of human TSH using dried blood samples. J Clin Endocrinol Metab. 1976 May;42(5):987–990. doi: 10.1210/jcem-42-5-987. [DOI] [PubMed] [Google Scholar]
  13. Pedersen S. S., Espersen F., Høiby N. Diagnosis of chronic Pseudomonas aeruginosa infection in cystic fibrosis by enzyme-linked immunosorbent assay. J Clin Microbiol. 1987 Oct;25(10):1830–1836. doi: 10.1128/jcm.25.10.1830-1836.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pedersen S. S., Jensen T., Høiby N., Koch C., Flensborg E. W. Management of Pseudomonas aeruginosa lung infection in Danish cystic fibrosis patients. Acta Paediatr Scand. 1987 Nov;76(6):955–961. doi: 10.1111/j.1651-2227.1987.tb17271.x. [DOI] [PubMed] [Google Scholar]
  15. Pedersen S. How to use a rotahaler. Arch Dis Child. 1986 Jan;61(1):11–14. doi: 10.1136/adc.61.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pier G. B. Pulmonary disease associated with Pseudomonas aeruginosa in cystic fibrosis: current status of the host-bacterium interaction. J Infect Dis. 1985 Apr;151(4):575–580. doi: 10.1093/infdis/151.4.575. [DOI] [PubMed] [Google Scholar]
  17. Shale D. J., Faux J. A. The evaluation of a quantitative enzyme-linked immunosorbent assay (ELISA) for anti-Aspergillus fumigatus IgG. J Immunol Methods. 1985 Mar 18;77(2):197–205. doi: 10.1016/0022-1759(85)90032-8. [DOI] [PubMed] [Google Scholar]

Articles from Archives of Disease in Childhood are provided here courtesy of BMJ Publishing Group

RESOURCES