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. 1997 Sep;179(18):5963–5966. doi: 10.1128/jb.179.18.5963-5966.1997

Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase.

L Zheng 1, R H White 1, D R Dean 1
PMCID: PMC179493  PMID: 9294461

Abstract

The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at high levels in Escherichia coli and purified. NifV is a homodimer that catalyzes the condensation of acetyl coenzyme A (acetyl-CoA) and alpha-ketoglutarate. Although alpha-ketoglutarate supports the highest level of activity, NifV will also catalyze the condensation of acetyl-CoA and certain other keto acids. E. coli cells in which a high level of nifV expression is induced excrete homocitrate into the growth medium.

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Selected References

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