Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1986 Mar;29(3):456–460. doi: 10.1128/aac.29.3.456

Isolation, characterization, and mode of action on Escherichia coli strains of microcin D93.

J L Martínez, J C Pérez-Díaz
PMCID: PMC180413  PMID: 3521486

Abstract

Microcin D93 is an antibiotic substance produced by Escherichia coli strains which harbor the 5.5-kilobase plasmid pMccD93. Its production is unaffected by the use of different carbon and ammonia sources, different phosphate concentrations, or mitomycin C. We developed a method for purifying this microcin based on gel permeation chromatography and reverse-phase high-pressure liquid chromatography. The antibiotic appears to be a small, hydrophilic, basic peptide, active on E. coli and Proteus, Citrobacter, and Pseudomonas species and much more active on recA strains than on their isogenic wild type. Diminution of the rate of DNA biosynthesis without any apparent effect on other macromolecules appears to be a primary effect in the action of microcin D93.

Full text

PDF
457

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguilar A., Baquero F., Martínez J. L., Asensio C. Microcin 15n: a second antibiotic from Escherichia coli LP15. J Antibiot (Tokyo) 1983 Mar;36(3):325–327. doi: 10.7164/antibiotics.36.325. [DOI] [PubMed] [Google Scholar]
  2. Aguilar A., Pérez-Díaz J. C., Baquero F., Asensio C. Microcin 15m from Escherichia coli: mechanism of antibiotic action. Antimicrob Agents Chemother. 1982 Mar;21(3):381–386. doi: 10.1128/aac.21.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Asensio C., Pérez-Díaz J. C. A new family of low molecular weight antibiotics from enterobacteria. Biochem Biophys Res Commun. 1976 Mar 8;69(1):7–14. doi: 10.1016/s0006-291x(76)80264-1. [DOI] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  5. Duro A. F., Serrano R., Asensio C. Effect of the antibiotic microcin 140 on the ATP level and amino acid transport of Escherichia coli. Biochem Biophys Res Commun. 1979 May 14;88(1):297–304. doi: 10.1016/0006-291x(79)91729-7. [DOI] [PubMed] [Google Scholar]
  6. Fry D. E., Garrison R. N., Trachtenberg L., Polk H. C., Jr Bacterial inoculum and the activity of antimicrobial agents. Surg Gynecol Obstet. 1985 Feb;160(2):105–108. [PubMed] [Google Scholar]
  7. Garcia-Bustos J. F., Pezzi N., Mendez E. Structure and mode of action of microcin 7, an antibacterial peptide produced by Escherichia coli. Antimicrob Agents Chemother. 1985 May;27(5):791–797. doi: 10.1128/aac.27.5.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. García-Bustos J. F., Pezzi N., Asensio C. Microcin 7: purification and properties. Biochem Biophys Res Commun. 1984 Mar 15;119(2):779–785. doi: 10.1016/s0006-291x(84)80318-6. [DOI] [PubMed] [Google Scholar]
  9. Hollstein M., McCann J., Angelosanto F. A., Nichols W. W. Short-term tests for carcinogens and mutagens. Mutat Res. 1979 Sep;65(3):133–226. doi: 10.1016/0165-1110(79)90014-9. [DOI] [PubMed] [Google Scholar]
  10. Kreuzer K., Pratt C., Torriani A. Genetic analysis of regulatory mutants of alkaline phosphatase of E. coli. Genetics. 1975 Nov;81(3):459–468. doi: 10.1093/genetics/81.3.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yang C. C., Konisky J. Colicin V-treated Escherichia coli does not generate membrane potential. J Bacteriol. 1984 May;158(2):757–759. doi: 10.1128/jb.158.2.757-759.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. de Lorenzo V. Isolation and characterization of microcin E492 from Klebsiella pneumoniae. Arch Microbiol. 1984 Sep;139(1):72–75. doi: 10.1007/BF00692715. [DOI] [PubMed] [Google Scholar]
  14. de Lorenzo V., Pugsley A. P. Microcin E492, a low-molecular-weight peptide antibiotic which causes depolarization of the Escherichia coli cytoplasmic membrane. Antimicrob Agents Chemother. 1985 Apr;27(4):666–669. doi: 10.1128/aac.27.4.666. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES