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. 1981 May;19(5):766–772. doi: 10.1128/aac.19.5.766

Aminocyclitol-modifying enzymes specified by chromosomal genes in Staphylococcus aureus.

F H Kayser, F Homberger, M Devaud
PMCID: PMC181519  PMID: 6271046

Abstract

A genetic analysis of aminocyclitol resistance in two strains of Staphylococcus aureus was performed. Resistance of strain FK170, isolated in Zurich, was due to the production of a 3'-phosphotransferase [APH(3')]. Strain 5532, isolated in London, produced a 2"-phosphotransferase [APH(2")] and a 6'-N-acetyltransferase [AAC(6')]. Plasmid deoxyribonucleic acid (DNA) was isolated by isopycnic centrifugation from the two parent strains, as well as from susceptible variants and from resistant transductants of both strains. Comparative analysis of plasmid and from resistant transductants of both strains. Comparative analysis of plasmid DNA by centrifugation in sucrose gradients revealed that strain FK170 harbored a 2.7-megadalton tetracycline R-plasmid and a 36-megadalton cryptic plasmid. Strain 5532 contained an 18.5-megadalton penicillinase plasmid. No evidence for plasmid location of the markers for aminocyclitol resistance could be obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asheshov E. H. Chromosomal location of the genetic elements controlling penicillinase production in a strain of Staphylococcus aureus. Nature. 1966 May 21;210(5038):804–806. doi: 10.1038/210804a0. [DOI] [PubMed] [Google Scholar]
  2. Brown D. F., Kayser F. H., Biber J. Gentamicin resistance in Staphylococcus aureus. Lancet. 1976 Aug 21;2(7982):419–419. doi: 10.1016/s0140-6736(76)92435-1. [DOI] [PubMed] [Google Scholar]
  3. Chopra I., Bennett P. M., Lacey R. W. A variety of Staphylococcal plasmids present as multiple copies. J Gen Microbiol. 1973 Dec;79(2):343–345. doi: 10.1099/00221287-79-2-343. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  5. Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies J., Brzezinska M., Benveniste R. The problems of drug-resistant pathogenic bacteria. R factors: biochemical mechanisms of resistance to aminoglycoside antibiotics. Ann N Y Acad Sci. 1971 Jun 11;182:226–233. doi: 10.1111/j.1749-6632.1971.tb30659.x. [DOI] [PubMed] [Google Scholar]
  7. Dowding J. E. Mechanisms of gentamicin resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1977 Jan;11(1):47–50. doi: 10.1128/aac.11.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eckhardt T. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978 Sep;1(4):584–588. doi: 10.1016/0147-619x(78)90016-1. [DOI] [PubMed] [Google Scholar]
  9. Ericsson H. M., Sherris J. C. Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;217(Suppl):1+–1+. [PubMed] [Google Scholar]
  10. Falkow S., Tompkins L. S., Silver R. P., Guerry P., Le Blanc D. J. The problems of drug-resistant pathogenic bacteria. The replication of R-factor DNA in Escherichia coli K-12 following conjugation. Ann N Y Acad Sci. 1971 Jun 11;182:153–171. doi: 10.1111/j.1749-6632.1971.tb30654.x. [DOI] [PubMed] [Google Scholar]
  11. Hudson B., Clayton D. A., Vinograd J. Complex mitochondrial DNA. Cold Spring Harb Symp Quant Biol. 1968;33:435–442. doi: 10.1101/sqb.1968.033.01.050. [DOI] [PubMed] [Google Scholar]
  12. Jaffe H. W., Sweeney H. M., Nathan C., Weinstein R. A., Kabins S. A., Cohen S. Identity and interspecific transfer of gentamicin-resistance plasmids in Staphylococcus aureus and Staphylococcus epidermidis. J Infect Dis. 1980 Jun;141(6):738–747. doi: 10.1093/infdis/141.6.738. [DOI] [PubMed] [Google Scholar]
  13. Kayser F. H., Wüst J., Corrodi P. Transduction and elimination of resistance determinants in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1972 Sep;2(3):217–223. doi: 10.1128/aac.2.3.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kayser F. H., Wüst J., Santanam P. Genetic and molecular characterisation of resistance determinants in methicillin-resistant Staphylococcus-aureus. J Med Microbiol. 1976 May;9(2):137–148. doi: 10.1099/00222615-9-2-137. [DOI] [PubMed] [Google Scholar]
  15. Lacey R. W., Grinsted J. Genetic analysis of methicillin-resistant strains of Staphylococcus aureus; evidence for their evolution from a single clone. J Med Microbiol. 1973 Nov;6(4):511–526. doi: 10.1099/00222615-6-4-511. [DOI] [PubMed] [Google Scholar]
  16. Le Goffic F., Martel A., Capmau M. L., Baca B., Goebel P., Chardon H., Soussy C. J., Duval J., Bouanchaud D. H. New plasmid-mediated nucleotidylation of aminoglycoside antibiotics in Staphlococcus aureus. Antimicrob Agents Chemother. 1976 Aug;10(2):258–264. doi: 10.1128/aac.10.2.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lindberg M., Novick R. P. Plasmid-specific transformation in Staphylococcus aureus. J Bacteriol. 1973 Jul;115(1):139–145. doi: 10.1128/jb.115.1.139-145.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Martel A., Moreau N., Capmau M. L., Soussy C. J., Duval J. 2"-O-phosphorylation of gentamicin components by a Staphylococcus aureus strain carrying a plasmid. Antimicrob Agents Chemother. 1977 Jul;12(1):26–30. doi: 10.1128/aac.12.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  20. NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Novick R. P., Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. doi: 10.1111/j.1749-6632.1971.tb30664.x. [DOI] [PubMed] [Google Scholar]
  22. Novick R. P., Edelman I., Schwesinger M. D., Gruss A. D., Swanson E. C., Pattee P. A. Genetic translocation in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1979 Jan;76(1):400–404. doi: 10.1073/pnas.76.1.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Novick R. P. Penicillinase plasmids of Staphylococcus aureus. Fed Proc. 1967 Jan-Feb;26(1):29–38. [PubMed] [Google Scholar]
  24. Pattee P. A., Thompson N. E., Haubrich D., Novick R. P. Chromosomal map locations of integrated plasmids and related elements in Staphylococcus aureus. Plasmid. 1977 Nov;1(1):38–51. doi: 10.1016/0147-619x(77)90007-5. [DOI] [PubMed] [Google Scholar]
  25. Porthouse A., Brown D. F., Smith R. G., Rogers T. Gentamicin resistance in Staphylococcus aureus. Lancet. 1976 Jan 3;1(7949):20–21. doi: 10.1016/s0140-6736(76)92912-3. [DOI] [PubMed] [Google Scholar]
  26. Poston S. M. Cellular location of the genes controlling penicillinase production and resistance to streptomycin and tetracycline in a strain of Staphylococcus aureus. Nature. 1966 May 21;210(5038):802–804. doi: 10.1038/210802a0. [DOI] [PubMed] [Google Scholar]
  27. Rosendorf L. L., Kayser F. H. Transduction and plasmid deoxyribonucleic acid analysis in a multiply antibiotic-resistant strain of Staphylococcus epidermidis. J Bacteriol. 1974 Nov;120(2):679–686. doi: 10.1128/jb.120.2.679-686.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Santanam P., Kayser F. H. Tobramycin adenylyltransferase: a new aminoglycoside-inactivating enzyme from Staphylococcus epidermidis. J Infect Dis. 1976 Aug;134 (Suppl):S33–S39. doi: 10.1093/infdis/134.supplement_1.s33. [DOI] [PubMed] [Google Scholar]
  29. Soussy C. J., Bouanchaud D. H., Fouace J., Dublanchet A., Duval J. A gentamycin resistance plasmid in Staphylococcus aureus. Ann Microbiol (Paris) 1975 Jul-Aug;126B(1):91–94. [PubMed] [Google Scholar]
  30. Wood D. O., Carter M. J., Best G. K. Plasmid-mediated resistance to gentamicin in Staphylococcus aureus. Antimicrob Agents Chemother. 1977 Oct;12(4):513–517. doi: 10.1128/aac.12.4.513. [DOI] [PMC free article] [PubMed] [Google Scholar]

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