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. 1993 May;59(5):1627–1634. doi: 10.1128/aem.59.5.1627-1634.1993

Similarity between Copper Resistance Genes from Xanthomonas campestris and Pseudomonas syringae

Andreas E Voloudakis 1, Carol L Bender 1, Donald A Cooksey 1,*
PMCID: PMC182129  PMID: 16348942

Abstract

Plasmid-borne copper resistance genes from copper-resistant strains of Xanthomonas campestris pv. vesicatoria from California, Florida, and Oklahoma shared structural similarities. A strain of X. campestris pv. campestris also contained plasmid-borne copper resistance genes similar to the resistance genes from X. campestris pv. vesicatoria. Furthermore, a region of the copper resistance genes from X. campestris pv. vesicatoria 07882 hybridized with copA, the first gene of the copper resistance operon (cop) of Pseudomonas syringae pv. tomato. A copper-inducible protein of similar size to CopA was detected by Western blot (immunoblot) analysis from the wild-type strain 07882 and from the cloned copper resistance genes of 07882 introduced into a copper-sensitive strain of X. campestris pv. vesicatoria. A low level of hybridization was observed with chromosomal DNA from other xanthomonads when the copper resistance genes from strain 07882 were used as probes.

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Selected References

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