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. 1993 Jun;59(6):1972–1974. doi: 10.1128/aem.59.6.1972-1974.1993

Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils

Charles C Young 1,†, Robert L Burghoff 1,‡, Lois G Keim 1, Vera Minak-Bernero 1, James R Lute 1, Stephen M Hinton 1,*
PMCID: PMC182198  PMID: 16348974

Abstract

This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards the electrophoretic mobility of denaturing phenolic compounds so that they do not comigrate with nucleic acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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