Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1993 Jul;59(7):2320–2322. doi: 10.1128/aem.59.7.2320-2322.1993

Cell Density and Growth Phase as Factors in the Resistance of a Biofilm of Pseudomonas aeruginosa (ATCC 27853) to Iodine

Matthew L Brown 1,*, Joseph J Gauthier 1
PMCID: PMC182276  PMID: 16349001

Abstract

Previous studies have shown that biofilms exhibit enhanced resistance to iodine. Investigations were conducted to determine the relative importance of growth phase versus cell density on biofilm resistance of Pseudomonas aeruginosa (ATCC 27853) to iodine. Cell density is a contributing factor to resistance, whereas growth to the stationary phase is not sufficient to achieve resistance.

Full text

PDF
2322

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. L., Holland B. W., Carr J. K., Bond W. W., Favero M. S. Effect of disinfectants on pseudomonads colonized on the interior surface of PVC pipes. Am J Public Health. 1990 Jan;80(1):17–21. doi: 10.2105/ajph.80.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson R. L. Iodophor antiseptics: intrinsic microbial contamination with resistant bacteria. Infect Control Hosp Epidemiol. 1989 Oct;10(10):443–446. doi: 10.1086/645918. [DOI] [PubMed] [Google Scholar]
  3. Anderson R. L., Vess R. W., Carr J. H., Bond W. W., Panlilio A. L., Favero M. S. Investigations of intrinsic Pseudomonas cepacia contamination in commercially manufactured povidone-iodine. Infect Control Hosp Epidemiol. 1991 May;12(5):297–302. doi: 10.1086/646342. [DOI] [PubMed] [Google Scholar]
  4. Anwar H., Dasgupta M. K., Costerton J. W. Testing the susceptibility of bacteria in biofilms to antibacterial agents. Antimicrob Agents Chemother. 1990 Nov;34(11):2043–2046. doi: 10.1128/aac.34.11.2043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown M. R., Collier P. J., Gilbert P. Influence of growth rate on susceptibility to antimicrobial agents: modification of the cell envelope and batch and continuous culture studies. Antimicrob Agents Chemother. 1990 Sep;34(9):1623–1628. doi: 10.1128/aac.34.9.1623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carson L. A., Petersen N. J., Favero M. S., Aguero S. M. Growth characteristics of atypical mycobacteria in water and their comparative resistance to disinfectants. Appl Environ Microbiol. 1978 Dec;36(6):839–846. doi: 10.1128/aem.36.6.839-846.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chai T. J. Characteristics of Escherichia coli grown in bay water as compared with rich medium. Appl Environ Microbiol. 1983 Apr;45(4):1316–1323. doi: 10.1128/aem.45.4.1316-1323.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Costerton J. W., Cheng K. J., Geesey G. G., Ladd T. I., Nickel J. C., Dasgupta M., Marrie T. J. Bacterial biofilms in nature and disease. Annu Rev Microbiol. 1987;41:435–464. doi: 10.1146/annurev.mi.41.100187.002251. [DOI] [PubMed] [Google Scholar]
  9. Costerton J. W., Irvin R. T., Cheng K. J. The bacterial glycocalyx in nature and disease. Annu Rev Microbiol. 1981;35:299–324. doi: 10.1146/annurev.mi.35.100181.001503. [DOI] [PubMed] [Google Scholar]
  10. Favero M. S., Drake C. H. Factors influencing the occurrence of high numbers of iodine-resistant bacteria in iodinated swimming pools. Appl Microbiol. 1966 Jul;14(4):627–635. doi: 10.1128/am.14.4.627-635.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goetz A., Muder R. R. Pseudomonas aeruginosa infections associated with use of povidone-iodine in patients receiving continuous ambulatory peritoneal dialysis. Infect Control Hosp Epidemiol. 1989 Oct;10(10):447–450. doi: 10.1086/645919. [DOI] [PubMed] [Google Scholar]
  12. LeChevallier M. W., Cawthon C. D., Lee R. G. Factors promoting survival of bacteria in chlorinated water supplies. Appl Environ Microbiol. 1988 Mar;54(3):649–654. doi: 10.1128/aem.54.3.649-654.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LeChevallier M. W., Cawthon C. D., Lee R. G. Inactivation of biofilm bacteria. Appl Environ Microbiol. 1988 Oct;54(10):2492–2499. doi: 10.1128/aem.54.10.2492-2499.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nichols W. W., Evans M. J., Slack M. P., Walmsley H. L. The penetration of antibiotics into aggregates of mucoid and non-mucoid Pseudomonas aeruginosa. J Gen Microbiol. 1989 May;135(5):1291–1303. doi: 10.1099/00221287-135-5-1291. [DOI] [PubMed] [Google Scholar]
  15. Pyle B. H., McFeters G. A. Iodine susceptibility of pseudomonads grown attached to stainless steel surfaces. Biofouling. 1990;2:113–120. doi: 10.1080/08927019009378137. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES