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. 1993 Sep;59(9):2945–2950. doi: 10.1128/aem.59.9.2945-2950.1993

Metabolism of Linoleic Acid or Mevalonate and 6-Pentyl-α-Pyrone Biosynthesis by Trichoderma Species

L Serrano-Carreon 1, Y Hathout 1, M Bensoussan 1, J-M Belin 1,*
PMCID: PMC182390  PMID: 16349040

Abstract

The understanding of the biosynthetic pathway of 6-pentyl-α-pyrone in Trichoderma species was achieved by using labelled linoleic acid or mevalonate as a tracer. Incubation of growing cultures of Trichoderma harzianum and T. viride with [U-14C]linoleic acid or [5-14C]sodium mevalonate revealed that both fungal strains were able to incorporate these labelled compounds (50 and 15%, respectively). Most intracellular radioactivity was found in the neutral lipid fraction. At the initial time of incubation, the radioactivity from [14C]linoleic acid was incorporated into 6-pentyl-α-pyrone more rapidly than that from [14C]mevalonate. No radioactivity incorporation was detected in 6-pentyl-α-pyrone when fungal cultures were incubated with [1-14C]linoleic acid. These results suggested that β-oxidation of linoleic acid was a probable main step in the biosynthetic pathway of 6-pentyl-α-pyrone in Trichoderma species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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