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. 1993 Sep;59(9):3123–3125. doi: 10.1128/aem.59.9.3123-3125.1993

Direct Quantification of Unadsorbed Viruses in Suspensions of Adsorbing Colloids with the Enzyme-Linked Immunosorbent Assay

D Dornai 1,*, U Mingelgrin 1, H Frenkel 1, M Bar-Joseph 1
PMCID: PMC182416  PMID: 16349051

Abstract

Quantification of two plant viruses in suspensions of homoionic Ca-bentonite was conducted by applying to the enzyme-linked immunosorbent assay plates either the virus-clay mixture (direct method) or the supernatant obtained after the clay was allowed to settle (classic method). Both methods showed a similar dependence of free virus content on clay concentration. A higher content at equilibrium was measured for both viruses by the direct method. The advantage of using the direct over the classic method is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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