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. 1993 Sep;59(9):3130–3133. doi: 10.1128/aem.59.9.3130-3133.1993

Genotypic Diversity among Strains of Bradyrhizobium japonicum Belonging to Serogroup 110

P van Berkum 1,*, S I Kotob 1, H Abdel Basit 1, S Salem 1, E M Gewaily 1, J S Angle 1
PMCID: PMC182418  PMID: 16349053

Abstract

Thirty-three strains of Bradyrhizobium japonicum within serogroup 110 were examined for genotypic diversity by using DNA-DNA hybridization analyses. The analysis of the DNA from 15 hydrogen-uptake-negative strains with the bradyrhizobial uptake hydrogenase probe pHU52 showed variation in degree of homology and restriction fragment length polymorphism of EcoRI-restricted DNA. Clustering analysis of the 33 strains on the basis of DNA-DNA hybridization analysis with four restriction enzymes and with the bradyrhizobial nodulation locus, pRJUT10, as probe indicated the existence of four groups of strains, which were less than 70% similar. Restriction digestion of genomic DNA with BamHI and DNA-DNA hybridization with pRJUT10 permitted classification of each of the strains according to a specific fingerprint pattern.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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