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. 1993 Oct;59(10):3502–3504. doi: 10.1128/aem.59.10.3502-3504.1993

Adaptation of Pseudomonas putida S12 to high concentrations of styrene and other organic solvents.

F J Weber 1, L P Ooijkaas 1, R M Schemen 1, S Hartmans 1, J A de Bont 1
PMCID: PMC182484  PMID: 8250572

Abstract

Pseudomonas putida S12 could adapt to grow on styrene in a two-phase styrene-water system. Acetate was toxic for P. putida S12, but cells were similarly able to adapt to higher acetate concentrations. Only by using these acetate-adapted cells was growth observed in the presence of supersaturating concentrations of toxic nonmetabolizable solvents such as toluene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cruden D. L., Wolfram J. H., Rogers R. D., Gibson D. T. Physiological properties of a Pseudomonas strain which grows with p-xylene in a two-phase (organic-aqueous) medium. Appl Environ Microbiol. 1992 Sep;58(9):2723–2729. doi: 10.1128/aem.58.9.2723-2729.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hartmans S., Smits J. P., van der Werf M. J., Volkering F., de Bont J. A. Metabolism of Styrene Oxide and 2-Phenylethanol in the Styrene-Degrading Xanthobacter Strain 124X. Appl Environ Microbiol. 1989 Nov;55(11):2850–2855. doi: 10.1128/aem.55.11.2850-2855.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hartmans S., van der Werf M. J., de Bont J. A. Bacterial degradation of styrene involving a novel flavin adenine dinucleotide-dependent styrene monooxygenase. Appl Environ Microbiol. 1990 May;56(5):1347–1351. doi: 10.1128/aem.56.5.1347-1351.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heipieper H. J., Diefenbach R., Keweloh H. Conversion of cis unsaturated fatty acids to trans, a possible mechanism for the protection of phenol-degrading Pseudomonas putida P8 from substrate toxicity. Appl Environ Microbiol. 1992 Jun;58(6):1847–1852. doi: 10.1128/aem.58.6.1847-1852.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ingram L. O. Changes in lipid composition of Escherichia coli resulting from growth with organic solvents and with food additives. Appl Environ Microbiol. 1977 May;33(5):1233–1236. doi: 10.1128/aem.33.5.1233-1236.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sheu C. W., Freese E. Effects of fatty acids on growth and envelope proteins of Bacillus subtilis. J Bacteriol. 1972 Aug;111(2):516–524. doi: 10.1128/jb.111.2.516-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sikkema J., Poolman B., Konings W. N., de Bont J. A. Effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes. J Bacteriol. 1992 May;174(9):2986–2992. doi: 10.1128/jb.174.9.2986-2992.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. de Smet M. J., Kingma J., Witholt B. The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli. Biochim Biophys Acta. 1978 Jan 4;506(1):64–80. doi: 10.1016/0005-2736(78)90435-2. [DOI] [PubMed] [Google Scholar]

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