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. 1991 Apr;57(4):1218–1222. doi: 10.1128/aem.57.4.1218-1222.1991

Development and application of new positively charged filters for recovery of bacteriophages from water.

J J Borrego 1, R Cornax 1, D R Preston 1, S R Farrah 1, B McElhaney 1, G Bitton 1
PMCID: PMC182871  PMID: 2059044

Abstract

Electronegative and electropositive filters were compared for the recovery of indigenous bacteriophages from water samples, using the VIRADEL technique. Fiber glass and diatomaceous earth filters displayed low adsorption and recovery, but an important increase of the adsorption percentage was observed when the filters were treated with cationic polymers (about 99% adsorption). A new methodology of virus elution was developed in this study, consisting of the slow passage of the eluent through the filter, thus increasing the contact time between eluent and virus adsorbed on the filters. The use of this technique allows a maximum recovery of 71.2% compared with 46.7% phage recovery obtained by the standard elution procedure. High percentages (over 83%) of phage adsorption were obtained with different filters from 1-liter aliquots of the samples, except for Virosorb 1-MDS filters (between 1.6 and 32% phage adsorption). Phage recovery by using the slow passing of the eluent depended on the filter type, with recovery ranging between 1.6% for Virosorb 1-MDS filters treated with polyethyleneimine and 103.2% for diatomaceous earth filters treated with 0.1% Nalco.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang L. T., Farrah S. R., Bitton G. Positively charged filters for virus recovery from wastewater treatment plant effluents. Appl Environ Microbiol. 1981 Nov;42(5):921–924. doi: 10.1128/aem.42.5.921-924.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Farrah S. R., Girard M. A., Toranzos G. A., Preston D. R. Adsorption of viruses to diatomaceous earth modified by in situ precipitation of metallic salts. Z Gesamte Hyg. 1988 Sep;34(9):520–521. [PubMed] [Google Scholar]
  3. Gerba C. P., Farrah S. R., Goyal S. M., Wallis C., Melnick J. L. Concentration of enteroviruses from large volumes of tap water, treated sewage, and seawater. Appl Environ Microbiol. 1978 Mar;35(3):540–548. doi: 10.1128/aem.35.3.540-548.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goyal S. M., Zerda K. S., Gerba C. P. Concentration of coliphages from large volumes of water and wastewater. Appl Environ Microbiol. 1980 Jan;39(1):85–91. doi: 10.1128/aem.39.1.85-91.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Havelaar A. H., Hogeboom W. M. Factors affecting the enumeration of coliphages in sewage and sewage-polluted waters. Antonie Van Leeuwenhoek. 1983 Nov;49(4-5):387–397. doi: 10.1007/BF00399318. [DOI] [PubMed] [Google Scholar]
  6. Rose J. B., Singh S. N., Gerba C. P., Kelley L. M. Comparison of microporous filters for concentration of viruses from wastewater. Appl Environ Microbiol. 1984 May;47(5):989–992. doi: 10.1128/aem.47.5.989-992.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sabatino C. M., Maier S. Differential inactivation of three bacteriophages by acid and alkaline pH used in the membrane adsorption-elution method of virus recovery. Can J Microbiol. 1980 Dec;26(12):1403–1407. doi: 10.1139/m80-233. [DOI] [PubMed] [Google Scholar]
  8. Seeley N. D., Primrose S. B. Concentration of bacteriophages from natural waters. J Appl Bacteriol. 1979 Feb;46(1):103–116. doi: 10.1111/j.1365-2672.1979.tb02587.x. [DOI] [PubMed] [Google Scholar]
  9. Seeley N. D., Primrose S. B. The isolation of bacteriophages from the environment. J Appl Bacteriol. 1982 Aug;53(1):1–17. doi: 10.1111/j.1365-2672.1982.tb04729.x. [DOI] [PubMed] [Google Scholar]
  10. Shields P. A., Farrah S. R. Concentration of viruses in beef extract by flocculation with ammonium sulfate. Appl Environ Microbiol. 1986 Jan;51(1):211–213. doi: 10.1128/aem.51.1.211-213.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Singh S. N., Gerba C. P. Concentration of coliphage from water and sewage with charge-modified filter aid. Appl Environ Microbiol. 1983 Jan;45(1):232–237. doi: 10.1128/aem.45.1.232-237.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sobsey M. D., Glass J. S. Influence of water quality on enteric virus concentration by microporous filter methods. Appl Environ Microbiol. 1984 May;47(5):956–960. doi: 10.1128/aem.47.5.956-960.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sobsey M. D., Glass J. S. Poliovirus concentration from tap water with electropositive adsorbent filters. Appl Environ Microbiol. 1980 Aug;40(2):201–210. doi: 10.1128/aem.40.2.201-210.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sobsey M. D., Jones B. L. Concentration of poliovirus from tap water using positively charged microporous filters. Appl Environ Microbiol. 1979 Mar;37(3):588–595. doi: 10.1128/aem.37.3.588-595.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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