Abstract
Rhizobitoxine is a phytotoxin synthesized by some strains of the legume symbiont genus Bradyrhizobium and the plant pathogen Pseudomonas andropogonis. We demonstrate here a new enzymatic assay which is 100-fold more sensitive than previous assays and can detect as little as 1.0 pmol of rhizobitoxine. The assay is based on the inhibition of Salmonella typhimurium beta-cystathionase by rhizobitoxine. Interestingly, beta-cystathionase from Bradyrhizobium japonicum is insensitive to rhizobitoxine at concentrations lower than 75 microM.
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- Fuhrmann J. Symbiotic effectiveness of indigenous soybean bradyrhizobia as related to serological, morphological, rhizobitoxine, and hydrogenase phenotypes. Appl Environ Microbiol. 1990 Jan;56(1):224–229. doi: 10.1128/aem.56.1.224-229.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Giovanelli J., Owens L. D., Mudd S. H. Mechanism of inhibition of spinach beta-cystathionase by rhizobitoxine. Biochim Biophys Acta. 1971 Mar 10;227(3):671–684. doi: 10.1016/0005-2744(71)90016-7. [DOI] [PubMed] [Google Scholar]
- Kaplan M. M., Flavin M. Cystathionine gamma-synthetase of Salmonella. Structural properties of a new enzyme in bacterial methionine biosynthesis. J Biol Chem. 1966 Dec 25;241(24):5781–5789. [PubMed] [Google Scholar]
- Owens L. D., Guggenheim S., Hilton J. L. Rhizobium-synthesized phytototoxin: an inhibitor of beta-cystathionase in Salmonella typhimurium. Biochim Biophys Acta. 1968 May;158(2):219–225. doi: 10.1016/0304-4165(68)90134-7. [DOI] [PubMed] [Google Scholar]
- Owens L. D., Wright D. A. Production of the Soybean-Chlorosis Toxin by Rhizobium japonicum in Pure Culture. Plant Physiol. 1965 Sep;40(5):931–933. doi: 10.1104/pp.40.5.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Owens L. D., Wright D. A. Rhizobial-Induced Chlorosis in Soybeans: Isolation, Production in Nodules, and Varietal Specificity of the Toxin. Plant Physiol. 1965 Sep;40(5):927–930. doi: 10.1104/pp.40.5.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peters N. K., Crist-Estes D. K. Nodule formation is stimulated by the ethylene inhibitor aminoethoxyvinylglycine. Plant Physiol. 1989 Oct;91(2):690–693. doi: 10.1104/pp.91.2.690. [DOI] [PMC free article] [PubMed] [Google Scholar]