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. 1982 Jul;22(1):120–127. doi: 10.1128/aac.22.1.120

Effects of sublethal concentrations of antimicrobial agents on the hemagglutination, adhesion, and ultrastructure of pyelonephritogenic Escherichia coli strains.

V Väisänen, K Lounatmaa, T K Korhonen
PMCID: PMC183684  PMID: 6751220

Abstract

The effects of trimethoprim, sulfadiazine, sulfamethoxazole, and sulfathiazole on the hemagglutination and adhesion by three Escherichia coli strains were studied. The strains were isolated from the urine of patients with acute pyelonephritis and carried P antigen-recognizing fimbriae (P fimbriae). At antimicrobial concentrations of 12.5 to 50% of the minimal inhibitory concentration, the ability of the bacteria to agglutinate human erythrocytes and to adhere to human buccal cells was markedly reduced. This reduction corresponded to a decrease in the number of P fimbriae per cell, suggesting that the antimicrobial agents decreased adhesion and hemagglutination by interfering with the formation of fimbriae. No major changes were observed in the outer membrane protein pattern of trimethoprim-treated cells, whereas freeze-fracture electron micrographs showed deorganization of both the cytoplasmic and outer membranes in bacteria opposed to sublethal concentrations of trimethoprim.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bamford D. H., Palva E. T., Lounatmaa K. Ultrastructure and life cycle of the lipid-containing bacteriophage phi 6. J Gen Virol. 1976 Aug;32(2):249–259. doi: 10.1099/0022-1317-32-2-249. [DOI] [PubMed] [Google Scholar]
  2. Eden C. S., Eriksson B., Hanson L. A. Adhesion of Escherichia coli to human uroepithelial cells in vitro. Infect Immun. 1977 Dec;18(3):767–774. doi: 10.1128/iai.18.3.767-774.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Evans D. G., Silver R. P., Evans D. J., Jr, Chase D. G., Gorbach S. L. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans. Infect Immun. 1975 Sep;12(3):656–667. doi: 10.1128/iai.12.3.656-667.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hagberg L., Jodal U., Korhonen T. K., Lidin-Janson G., Lindberg U., Svanborg Edén C. Adhesion, hemagglutination, and virulence of Escherichia coli causing urinary tract infections. Infect Immun. 1981 Feb;31(2):564–570. doi: 10.1128/iai.31.2.564-570.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kasanen A., Toivanen P., Sourander L., Kaarsalo E., Aantaa S. Trimethoprim in the treatment and long-term control of urinary tract infection. Scand J Infect Dis. 1974;6(1):91–96. doi: 10.3109/inf.1974.6.issue-1.15. [DOI] [PubMed] [Google Scholar]
  6. Korhonen T. K., Leffler H., Svanborg Edén C. Binding specificity of piliated strains of Escherichia coli and Salmonella typhimurium to epithelial cells, saccharomyces cerevisiae cells, and erythrocytes. Infect Immun. 1981 May;32(2):796–804. doi: 10.1128/iai.32.2.796-804.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Korhonen T. K., Väisänen V., Saxén H., Hultberg H., Svenson S. B. P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains. Infect Immun. 1982 Jul;37(1):286–291. doi: 10.1128/iai.37.1.286-291.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Källenius G., Svenson S., Möllby R., Cedergren B., Hultberg H., Winberg J. Structure of carbohydrate part of receptor on human uroepithelial cells for pyelonephritogenic Escherichia coli. Lancet. 1981 Sep 19;2(8247):604–606. doi: 10.1016/s0140-6736(81)92743-4. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Lounatmaa K., Mäkelä P. H., Sarvas M. Effect of polymyxin on the ultrastructure of the outer membrane of wild-type and polymyxin-resistant strain of Salmonella. J Bacteriol. 1976 Sep;127(3):1400–1407. doi: 10.1128/jb.127.3.1400-1407.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lounatmaa K., Nanninga N. Effect of polymyxin on the outer membrane of Salmonella typhimurium: freeze-fracture studies. J Bacteriol. 1976 Nov;128(2):665–667. doi: 10.1128/jb.128.2.665-667.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Müller H. E. Uber das unterschiedliche pathogenetische Verhalten in 171 Salmonella-Serotypen aufgrund ihres Vorkommens in Futtermitteln, Nutztieren und beim Menschen. Infection. 1978;6(3):121–129. doi: 10.1007/BF01642260. [DOI] [PubMed] [Google Scholar]
  13. Ofek I., Beachey E. H., Eisenstein B. I., Alkan M. L., Sharon N. Suppression of bacterial adherence by subminimal inhibitory concentrations of beta-lactam and aminoglycoside antibiotics. Rev Infect Dis. 1979 Sep-Oct;1(5):832–837. doi: 10.1093/clinids/1.5.832. [DOI] [PubMed] [Google Scholar]
  14. Palva E. T. Major outer membrane protein in Salmonella typhimurium induced by maltose. J Bacteriol. 1978 Oct;136(1):286–294. doi: 10.1128/jb.136.1.286-294.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sandberg T., Stenqvist K., Svanborg-Edén C. Effects of subminimal inhibitory concentrations of ampicillin, chloramphenicol, and nitrofurantoin on the attachment of Escherichia coli to human uroepithelial cells in vitro. Rev Infect Dis. 1979 Sep-Oct;1(5):838–844. doi: 10.1093/clinids/1.5.838. [DOI] [PubMed] [Google Scholar]
  16. Vosbeck K., Handschin H., Menge E. B., Zak O. Effects of subminimal inhibitory concentrations of antibiotics on adhesiveness of Escherichia coli in vitro. Rev Infect Dis. 1979 Sep-Oct;1(5):845–851. doi: 10.1093/clinids/1.5.845. [DOI] [PubMed] [Google Scholar]
  17. Väisänen V., Elo J., Tallgren L. G., Siitonen A., Mäkelä P. H., Svanborg-Edén C., Källenius G., Svenson S. B., Hultberg H., Korhonen T. Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet. 1981 Dec 19;2(8260-61):1366–1369. doi: 10.1016/s0140-6736(81)92796-3. [DOI] [PubMed] [Google Scholar]
  18. van Gool A. P., Nanninga N. Fracture faces in the cell envelope of Escherichia coli. J Bacteriol. 1971 Oct;108(1):474–481. doi: 10.1128/jb.108.1.474-481.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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