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. 1982 Aug;22(2):204–207. doi: 10.1128/aac.22.2.204

Conjugative transfer of R-plasmids from Streptococcus faecalis to Staphylococcus aureus.

D R Schaberg, D B Clewell, L Glatzer
PMCID: PMC183711  PMID: 6821454

Abstract

R-plasmids originally isolated from Streptococcus pyogenes(pAC1,pAM15346), Streptococcus agalactiae(pIP501), and Streptococcus faecalis(pAM beta 1) were shown to be self-transferable on filter membranes from S. faecalis JH2-2 to Staphylococcus aureus recipients. The nonconjugative plasmid pAM alpha 1 was mobilized into S. aureus by pAM beta 1. Once in S. aureus, conjugative R-plasmids could be transferred to a second S. aureus recipient or back into S. faecalis. Determinants for chloramphenicol, clindamycin, erythromycin, and tetracycline resistances present on these streptococcal plasmids were expressed in S. aureus. Agarose gel electrophoresis, dye-buoyant centrifugation, and restriction endonuclease digestion showed that the plasmids were maintained intact as self-replicating elements in S. aureus recipients.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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