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. 1991 Dec;57(12):3405–3409. doi: 10.1128/aem.57.12.3405-3409.1991

Construction of a Bacteriophage-Resistant Derivative of Lactococcus lactis subsp. lactis 425A by Using the Conjugal Plasmid pNP40

Aidan Harrington 1, Colin Hill 1,*
PMCID: PMC183987  PMID: 16348595

Abstract

Lactococcus lactis subsp. lactis 425A is an atypical strain which excretes a high concentration of α-acetolactate when grown in milk. The conjugative lactococcal plasmid pNP40, which encodes phage and nisin resistance, was introduced to strain 425A by conjugation, using resistance to phage and nisin as a selection. No phage-nisin resistance mutants were encountered. Transconjugants display complete resistance at both 21 and 39°C to those phage previously identified as lytic for 425A. Transconjugants lose their resistance characteristics when spontaneously cured of pNP40. The commercially important property of 425A—production of high levels of α-acetolactic acid—is unaffected by the presence of pNP40.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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