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. 1989 May;55(5):1117–1121. doi: 10.1128/aem.55.5.1117-1121.1989

Competitive Inhibition of Ferrous Iron Oxidation by Thiobacillus ferrooxidans by Increasing Concentrations of Cells

Isamu Suzuki 1,*, Hector M Lizama 1, Patrick D Tackaberry 1
PMCID: PMC184263  PMID: 16347904

Abstract

The oxidation of ferrous iron (Fe2+) to ferric iron (Fe3+) with dioxygen (O2) by various strains of Thiobacillus ferrooxidans was studied by measuring the rate of O2 consumption at various Fe2+ concentrations and cell concentrations. The apparent Km values for Fe2+ remained constant at different cell concentrations of laboratory strains ATCC 13661 and ATCC 19859 but increased with increasing cell concentrations of mine isolates SM-4 and SM-5. The latter results are explained by the competitive inhibition of the Fe2+-binding site of a cell by other cells in the reaction mixture. Possible mechanisms involving cell surface properties are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cobley J. G., Cox J. C. Energy conservation in acidophilic bacteria. Microbiol Rev. 1983 Dec;47(4):579–595. doi: 10.1128/mr.47.4.579-595.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Harrison A. P., Jr The acidophilic thiobacilli and other acidophilic bacteria that share their habitat. Annu Rev Microbiol. 1984;38:265–292. doi: 10.1146/annurev.mi.38.100184.001405. [DOI] [PubMed] [Google Scholar]
  3. Hooper A. B., DiSpirito A. A. In bacteria which grow on simple reductants, generation of a proton gradient involves extracytoplasmic oxidation of substrate. Microbiol Rev. 1985 Jun;49(2):140–157. doi: 10.1128/mr.49.2.140-157.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hutchins S. R., Davidson M. S., Brierley J. A., Brierley C. L. Microorganisms in reclamation of metals. Annu Rev Microbiol. 1986;40:311–336. doi: 10.1146/annurev.mi.40.100186.001523. [DOI] [PubMed] [Google Scholar]
  5. Ingledew W. J. Thiobacillus ferrooxidans. The bioenergetics of an acidophilic chemolithotroph. Biochim Biophys Acta. 1982 Nov 30;683(2):89–117. doi: 10.1016/0304-4173(82)90007-6. [DOI] [PubMed] [Google Scholar]
  6. Lundgren D. G., Silver M. Ore leaching by bacteria. Annu Rev Microbiol. 1980;34:263–283. doi: 10.1146/annurev.mi.34.100180.001403. [DOI] [PubMed] [Google Scholar]
  7. Suzuki I. Mechanisms of inorganic oxidation and energy coupling. Annu Rev Microbiol. 1974;28(0):85–101. doi: 10.1146/annurev.mi.28.100174.000505. [DOI] [PubMed] [Google Scholar]

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