Abstract
A micro-enzyme-linked immunosorbent assay (micro-ELISA) using the Salmonella-TEK screen kit was tested for the detection of Salmonella spp. in pure cultures as well as in 30 artificially contaminated food samples and in 45 naturally contaminated food samples. Different raw, fleshy foods and processed foods were used as test products. The artificially contaminated minced meat samples were preenriched in buffered peptone water, and after incubation, different selective enrichment broths were tested. The micro-ELISA optical density values after enrichment and isolation of the different broths were very analogous. The quickest method to detect Salmonella spp. in different foods is to enrich them with Salmosyst broth, which reduces the total analysis time to 31 h. The Salmonella-TEK kit for Salmonella spp. provides a promising test for the detection of Salmonella antigens in food even when they are present at a low concentration (1 to 5 CFU/25 g). The cross-reaction of the anti-Salmonella antibodies, especially to other gram-negative bacteria, is nil.
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Selected References
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- Anderson J. M., Hartman P. A. Direct immunoassay for detection of salmonellae in foods and feeds. Appl Environ Microbiol. 1985 May;49(5):1124–1127. doi: 10.1128/aem.49.5.1124-1127.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Araj G. F., Chugh T. D. Detection of Salmonella spp. in clinical specimens by capture enzyme-linked immunosorbent assay. J Clin Microbiol. 1987 Nov;25(11):2150–2153. doi: 10.1128/jcm.25.11.2150-2153.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Flowers R. S., Klatt M. J., Keelan S. L., Swaminathan B., Gehle W. D., Chandonnet H. E. Fluorescent enzyme immunoassay for rapid screening of Salmonella in foods: collaborative study. J Assoc Off Anal Chem. 1989 Mar-Apr;72(2):318–325. [PubMed] [Google Scholar]
- Flowers R. S., Klatt M. J., Robison B. J., Mattingly J. A., Gabis D. A., Silliker J. H. Enzyme immunoassay for detection of Salmonella in low-moisture foods: collaborative study. J Assoc Off Anal Chem. 1987 May-Jun;70(3):530–535. [PubMed] [Google Scholar]
- Ibrahim G. F., Lyons M. J., Walker R. A., Fleet G. H. Rapid detection of salmonellae by immunoassays with titanous hydroxide as the solid phase. Appl Environ Microbiol. 1985 Sep;50(3):670–675. doi: 10.1128/aem.50.3.670-675.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krysinski E. P., Heimsch R. C. Use of enzyme-labeled antibodies to detect Salmonella in foods. Appl Environ Microbiol. 1977 Apr;33(4):947–954. doi: 10.1128/aem.33.4.947-954.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Minnich S. A., Hartman P. A., Heimsch R. C. Enzyme immunoassay for detection of Salmonellae in foods. Appl Environ Microbiol. 1982 Apr;43(4):877–893. doi: 10.1128/aem.43.4.877-893.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robison B. J., Pretzman C. I., Mattingly J. A. Enzyme immunoassay in which a myeloma protein is used for detection of salmonellae. Appl Environ Microbiol. 1983 Jun;45(6):1816–1821. doi: 10.1128/aem.45.6.1816-1821.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sperber W. H., Deibel R. H. Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. Appl Microbiol. 1969 Apr;17(4):533–539. doi: 10.1128/am.17.4.533-539.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]