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. 1990 Apr;56(4):1128–1134. doi: 10.1128/aem.56.4.1128-1134.1990

Expression of Bacillus thuringiensis delta-endotoxin genes during vegetative growth.

A M Mettus 1, A Macaluso 1
PMCID: PMC184353  PMID: 2160219

Abstract

Bacillus thuringiensis delta-endotoxin (crystal protein) genes are normally expressed only during sporulation. It is possible to produce crystal protein during vegetative growth by placing B. thuringiensis crystal protein genes downstream of a strong vegetative promoter. By removing a possible transcriptional terminator of the tetracycline resistance gene of pBC16 and inserting a multiple cloning site, delta-endotoxin genes can be cloned downstream from the tetracycline resistance gene promoter. This construct allows for readthrough transcription from the strong vegetative promoter. Crystal protein is then produced during vegetative growth as well as during sporulation in both B. thuringiensis and Bacillus megaterium. This construct also allows for production of delta-endotoxin in B. thuringiensis strains that do not normally produce delta-endotoxin because of a defect in sporulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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