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. 1990 May;56(5):1413–1419. doi: 10.1128/aem.56.5.1413-1419.1990

Growth and Survival of Streptomycete Inoculants and Extent of Plasmid Transfer in Sterile and Nonsterile Soil

Elizabeth M H Wellington 1,*, Neil Cresswell 1, Venetia A Saunders 1
PMCID: PMC184420  PMID: 16348192

Abstract

The growth and survival of strains of Streptomyces lividans and S. violaceolatus in sterile and nonsterile soil was investigated by using inoculated soil microcosms run as batch systems. It was evident that, after an initial short mycelial growth phase of 2 to 3 days, sporulation occurred and inoculants survived as spores. The transfer of a high-copy-number, self-transmissible plasmid, pIJ673, was detected by using intra- and interspecific crosses. The initial detection of transconjugants correlated with the development of the mycelial state of the inoculants (as confirmed by scanning electron microscopy) after 2 days of incubation. Subsequent spread of the plasmid was attributed to spread within existing mycelium followed by sporulation. In natural soil, inoculant numbers remained constant or declined, but plasmid transfer was readily detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coughter J. P., Stewart G. J. Genetic exchange in the environment. Antonie Van Leeuwenhoek. 1989;55(1):15–22. doi: 10.1007/BF02309615. [DOI] [PubMed] [Google Scholar]
  2. Herron P. R., Wellington E. M. New method for extraction of streptomycete spores from soil and application to the study of lysogeny in sterile amended and nonsterile soil. Appl Environ Microbiol. 1990 May;56(5):1406–1412. doi: 10.1128/aem.56.5.1406-1412.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kendall K. J., Cohen S. N. Plasmid transfer in Streptomyces lividans: identification of a kil-kor system associated with the transfer region of pIJ101. J Bacteriol. 1987 Sep;169(9):4177–4183. doi: 10.1128/jb.169.9.4177-4183.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kieser T., Hopwood D. A., Wright H. M., Thompson C. J. pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet. 1982;185(2):223–228. doi: 10.1007/BF00330791. [DOI] [PubMed] [Google Scholar]
  5. Mazodier P., Petter R., Thompson C. Intergeneric conjugation between Escherichia coli and Streptomyces species. J Bacteriol. 1989 Jun;171(6):3583–3585. doi: 10.1128/jb.171.6.3583-3585.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rafii F., Crawford D. L. Transfer of conjugative plasmids and mobilization of a nonconjugative plasmid between Streptomyces strains on agar and in soil. Appl Environ Microbiol. 1988 Jun;54(6):1334–1340. doi: 10.1128/aem.54.6.1334-1340.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Stotzky G., Babich H. Survival of, and genetic transfer by, genetically engineered bacteria in natural environments. Adv Appl Microbiol. 1986;31:93–138. doi: 10.1016/s0065-2164(08)70440-4. [DOI] [PubMed] [Google Scholar]
  8. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J. Numerical classification of Streptomyces and related genera. J Gen Microbiol. 1983 Jun;129(6):1743–1813. doi: 10.1099/00221287-129-6-1743. [DOI] [PubMed] [Google Scholar]

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