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. 1990 Jun;56(6):1967–1970. doi: 10.1128/aem.56.6.1967-1970.1990

DNA Probe for Lactobacillus delbrueckii

Michèle Delley 1, Beat Mollet 1,*, Herbert Hottinger 1
PMCID: PMC184540  PMID: 16348233

Abstract

From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognizes L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an α-32P-labeled DNA probe.

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Selected References

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