Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Jun;56(6):1971–1973. doi: 10.1128/aem.56.6.1971-1973.1990

Purification and Properties of Catechol 1,2-Dioxygenase from Rhizobium leguminosarum biovar viceae USDA 2370

Yung Pin Chen 1, Charles R Lovell 1,*
PMCID: PMC184541  PMID: 16348234

Abstract

The catechol 1,2-dioxygenase of Rhizobium leguminosarum biovar viceae USDA 2370 was purified 296-fold, yielding a homogeneous preparation with a specific activity of 51.1 U mg of protein-1. The molecular weight of the native protein was 70,000, with two identical subunits of 34,500 and 1 g-atom of iron per mol. The optimum pH for catalytic activity was 9.0 to 9.5.

Full text

PDF
1972

Images in this article

1972Image
on p.1972

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown C. M., Dilworth M. J. Ammonia assimilation by rhizobium cultures and bacteroids. J Gen Microbiol. 1975 Jan;86(1):39–48. doi: 10.1099/00221287-86-1-39. [DOI] [PubMed] [Google Scholar]
  2. HAYAISHI O., KATAGIRI M., ROTHBERG S. Studies on oxygenases; pyrocatechase. J Biol Chem. 1957 Dec;229(2):905–920. [PubMed] [Google Scholar]
  3. Kojima Y., Fujisawa H., Nakazawa A., Nakazawa T., Kanetsuna F., Taniuchi H., Nozaki M., Hayaishi O. Studies on pyrocatechase. I. Purification and spectral properties. J Biol Chem. 1967 Jul 25;242(14):3270–3278. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. NAKAGAWA H., INOUE H., TAKEDA Y. CHARACTERISTICS OF CATECHOL OXYGENASE FROM BREVIBACTERIUM FUSCUM. J Biochem. 1963 Jul;54:65–74. doi: 10.1093/oxfordjournals.jbchem.a127748. [DOI] [PubMed] [Google Scholar]
  7. Nakai C., Nakazawa T., Nozaki M. Purification and properties of catechol 1,2-dioxygenase (pyrocatechase) from Pseudomonas putida mt-2 in comparison with that from Pseudomonas arvilla C-1. Arch Biochem Biophys. 1988 Dec;267(2):701–713. doi: 10.1016/0003-9861(88)90079-3. [DOI] [PubMed] [Google Scholar]
  8. Ono K., Nozaki M., Hayaishi O. Purification and some properties of protocatechuate 4,5-dioxygenase. Biochim Biophys Acta. 1970 Nov 11;220(2):224–238. doi: 10.1016/0005-2744(70)90008-2. [DOI] [PubMed] [Google Scholar]
  9. Stanier R. Y., Ornston L. N. The beta-ketoadipate pathway. Adv Microb Physiol. 1973;9(0):89–151. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES