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. 1990 Jun;56(6):1974–1976. doi: 10.1128/aem.56.6.1974-1976.1990

Improved assay for surface hydrophobic avidity of Candida albicans cells.

K C Hazen 1, W G LeMelle 1
PMCID: PMC184542  PMID: 2200343

Abstract

A simple method that distinguishes among hydrophobic avidity levels of highly hydrophobic isolates of the pathogenic fungus Candida albicans is described. This method involves mixing polystyrene microspheres at different concentrations with a constant concentration of yeast cells and plotting the data in accordance with the Langmuir isotherm equation. A 10-fold difference between the C. albicans isolates with the lowest and highest avidity (KH) values was found. This method may also demonstrate that surface hydrophobic sites with different avidities are present within a yeast cell population.

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1976

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Absolom D. R. Measurement of surface properties of phagocytes, bacteria, and other particles. Methods Enzymol. 1986;132:16–95. doi: 10.1016/s0076-6879(86)32004-4. [DOI] [PubMed] [Google Scholar]
  2. Antley P. P., Hazen K. C. Role of yeast cell growth temperature on Candida albicans virulence in mice. Infect Immun. 1988 Nov;56(11):2884–2890. doi: 10.1128/iai.56.11.2884-2890.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark W. B., Bammann L. L., Gibbons R. J. Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infect Immun. 1978 Mar;19(3):846–853. doi: 10.1128/iai.19.3.846-853.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hazen B. W., Hazen K. C. Dynamic expression of cell surface hydrophobicity during initial yeast cell growth and before germ tube formation of Candida albicans. Infect Immun. 1988 Sep;56(9):2521–2525. doi: 10.1128/iai.56.9.2521-2525.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hazen B. W., Hazen K. C. Modification and application of a simple, surface hydrophobicity detection method to immune cells. J Immunol Methods. 1988 Mar 16;107(2):157–163. doi: 10.1016/0022-1759(88)90214-1. [DOI] [PubMed] [Google Scholar]
  6. Hazen K. C. Participation of yeast cell surface hydrophobicity in adherence of Candida albicans to human epithelial cells. Infect Immun. 1989 Jul;57(7):1894–1900. doi: 10.1128/iai.57.7.1894-1900.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hazen K. C., Plotkin B. J., Klimas D. M. Influence of growth conditions on cell surface hydrophobicity of Candida albicans and Candida glabrata. Infect Immun. 1986 Oct;54(1):269–271. doi: 10.1128/iai.54.1.269-271.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kennedy M. J. Adhesion and association mechanisms of Candida albicans. Curr Top Med Mycol. 1988;2:73–169. doi: 10.1007/978-1-4612-3730-3_4. [DOI] [PubMed] [Google Scholar]

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