Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1983 Jan;23(1):142–150. doi: 10.1128/aac.23.1.142

Determination of minimum bactericidal concentrations of oxacillin for Staphylococcus aureus: influence and significance of technical factors.

P C Taylor, F D Schoenknecht, J C Sherris, E C Linner
PMCID: PMC184632  PMID: 6830204

Abstract

The minimum bactericidal concentration of oxacillin for Staphylococcus aureus was shown to be considerably influenced by technical and definitional factors, particularly by the survival of some organisms on the walls of test tubes and by the growth phase of the inoculum. Attention to technical detail greatly improved reproducibility, and log-phase cultures of all strains showed greater than 99.9% killing in 24 h, at or close to the minimum inhibitory concentration, including eight strains described as tolerant. Some strains showed the paradoxical phenomenon of having more survivors in higher concentrations above the minimum bactericidal concentration. An accepted reference minimum bactericidal concentration procedure is needed for establishing clinical correlates and for a review of endpoint criteria. Routine minimum bactericidal concentration tests on S. aureus should be interpreted with great caution.

Full text

PDF
142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry A. L., Lasner R. A. In-vitro methods for determining minimal lethal concentrations of antimicrobial agents. Am J Clin Pathol. 1979 Jan;71(1):88–92. doi: 10.1093/ajcp/71.1.88. [DOI] [PubMed] [Google Scholar]
  2. Best G. K., Best N. H., Koval A. V. Evidence for participation of autolysins in bactericidal action of oxacillin on Staphylococcus aureus. Antimicrob Agents Chemother. 1974 Dec;6(6):825–830. doi: 10.1128/aac.6.6.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Garrod L. P. Action of Penicillin on Bacteria. Br Med J. 1945 Jan 27;1(4386):107–110. doi: 10.1136/bmj.1.4386.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goessens W. H., Fontijne P., Michel M. F. Factors influencing detection of tolerance in Staphylococcus aureus. Antimicrob Agents Chemother. 1982 Sep;22(3):364–368. doi: 10.1128/aac.22.3.364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gwynn M. N., Webb T. L., Rolinson G. N. Regrowth of Pseudomonas aeruginosa and other bacteria after the bactericidal action of carbenicillin and other beta-lactam antibiotics. J Infect Dis. 1981 Sep;144(3):263–269. doi: 10.1093/infdis/144.3.263. [DOI] [PubMed] [Google Scholar]
  6. Ishida K., Guze P. A., Kalmanson G. M., Albrandt K., Guze L. B. Variables in demonstrating methicillin tolerance in Staphylococcus aureus strains. Antimicrob Agents Chemother. 1982 Apr;21(4):688–690. doi: 10.1128/aac.21.4.688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kim K. S., Anthony B. F. Importance of bacterial growth phase in determining minimal bactericidal concentrations of penicillin and methicillin. Antimicrob Agents Chemother. 1981 Jun;19(6):1075–1077. doi: 10.1128/aac.19.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mayhall C. G., Apollo E. Effect of storage and changes in bacterial growth phase and antibiotic concentrations on antimicrobial tolerance in Staphylococcus aureus. Antimicrob Agents Chemother. 1980 Nov;18(5):784–788. doi: 10.1128/aac.18.5.784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mayhall C. G., Medoff G., Marr J. J. Variation in the susceptibility of strains of Staphylococcus aureus to oxacillin, cephalothin, and gentamicin. Antimicrob Agents Chemother. 1976 Oct;10(4):707–712. doi: 10.1128/aac.10.4.707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murray P. R., Jorgensen J. H. Quantitative susceptibility test methods in major United States medical centers. Antimicrob Agents Chemother. 1981 Jul;20(1):66–70. doi: 10.1128/aac.20.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nelson R. E., Washington J. A., 2nd Paradoxic and tolerant effects of moxalactam on Staphylococcus aureus. J Infect Dis. 1981 Aug;144(2):178–178. doi: 10.1093/infdis/144.2.178. [DOI] [PubMed] [Google Scholar]
  12. Oakley C. Use of antibiotics. Endocarditis. Br Med J. 1978 Aug 12;2(6135):489–490. doi: 10.1136/bmj.2.6135.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pearson R. D., Steigbigel R. T., Davis H. T., Chapman S. W. Method of reliable determination of minimal lethal antibiotic concentrations. Antimicrob Agents Chemother. 1980 Nov;18(5):699–708. doi: 10.1128/aac.18.5.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Valdés-Dapena M. A., Nissim J. E., Arey J. B., Godleski J., Schaaf H. D., Haust M. D. Yellow pulmonary hyaline membranes. J Pediatr. 1976 Jul;89(1):128–132. doi: 10.1016/s0022-3476(76)80947-x. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES