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. 1990 Aug;56(8):2511–2516. doi: 10.1128/aem.56.8.2511-2516.1990

Tetrachloroethene transformation to trichloroethene and cis-1,2-dichloroethene by sulfate-reducing enrichment cultures.

D M Bagley 1, J M Gossett 1
PMCID: PMC184757  PMID: 2403257

Abstract

Tetrachloroethene, also known as perchloroethylene, was reductively dechlorinated to trichloroethene and cis-1,2-dichloroethene by laboratory sulfate-reducing enrichment cultures. The causative organism or group was not identified. However, tetrachloroethene was dechlorinated to trichloroethene in 50 mM bromoethane-sulfonate-inhibited enrichments and to trichloroethene and cis-1,2-dichloroethene in 3 mM fluoroacetate-inhibited enrichments. Overall transformation varied from 92% tetrachloroethene removal in 13 days to 22% removal in 65 days, depending on conditions of the inoculum, inhibitor used, and auxilliary substrate used. Neither lactate, acetate, methanol, isobutyric acid, valeric acid, isovaleric acid, hexanoic acid, succinic acid, nor hydrogen appeared directly to support tetrachloroethene dechlorination, although lactate-fed inocula demonstrated longer-term dechlorinating capability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bouwer E. J., McCarty P. L. Transformations of 1- and 2-carbon halogenated aliphatic organic compounds under methanogenic conditions. Appl Environ Microbiol. 1983 Apr;45(4):1286–1294. doi: 10.1128/aem.45.4.1286-1294.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ehrlich G. G., Goerlitz D. F., Bourell J. H., Eisen G. V., Godsy E. M. Liquid chromatographic procedure for fermentation product analysis in the identification of anaerobic bacteria. Appl Environ Microbiol. 1981 Nov;42(5):878–885. doi: 10.1128/aem.42.5.878-885.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fathepure B. Z., Boyd S. A. Dependence of tetrachloroethylene dechlorination on methanogenic substrate consumption by Methanosarcina sp. strain DCM. Appl Environ Microbiol. 1988 Dec;54(12):2976–2980. doi: 10.1128/aem.54.12.2976-2980.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fathepure B. Z., Nengu J. P., Boyd S. A. Anaerobic bacteria that dechlorinate perchloroethene. Appl Environ Microbiol. 1987 Nov;53(11):2671–2674. doi: 10.1128/aem.53.11.2671-2674.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Freedman D. L., Gossett J. M. Biological reductive dechlorination of tetrachloroethylene and trichloroethylene to ethylene under methanogenic conditions. Appl Environ Microbiol. 1989 Sep;55(9):2144–2151. doi: 10.1128/aem.55.9.2144-2151.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibson S. A., Suflita J. M. Extrapolation of biodegradation results to groundwater aquifers: reductive dehalogenation of aromatic compounds. Appl Environ Microbiol. 1986 Oct;52(4):681–688. doi: 10.1128/aem.52.4.681-688.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gunsalus R. P., Romesser J. A., Wolfe R. S. Preparation of coenzyme M analogues and their activity in the methyl coenzyme M reductase system of Methanobacterium thermoautotrophicum. Biochemistry. 1978 Jun 13;17(12):2374–2377. doi: 10.1021/bi00605a019. [DOI] [PubMed] [Google Scholar]
  8. Ingvorsen K., Zehnder A. J., Jørgensen B. B. Kinetics of Sulfate and Acetate Uptake by Desulfobacter postgatei. Appl Environ Microbiol. 1984 Feb;47(2):403–408. doi: 10.1128/aem.47.2.403-408.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lovley D. R., Klug M. J. Sulfate reducers can outcompete methanogens at freshwater sulfate concentrations. Appl Environ Microbiol. 1983 Jan;45(1):187–192. doi: 10.1128/aem.45.1.187-192.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shelton D. R., Tiedje J. M. Isolation and partial characterization of bacteria in an anaerobic consortium that mineralizes 3-chlorobenzoic Acid. Appl Environ Microbiol. 1984 Oct;48(4):840–848. doi: 10.1128/aem.48.4.840-848.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stevens T. O., Linkfield T. G., Tiedje J. M. Physiological characterization of strain DCB-1, a unique dehalogenating sulfidogenic bacterium. Appl Environ Microbiol. 1988 Dec;54(12):2938–2943. doi: 10.1128/aem.54.12.2938-2943.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vogel T. M., McCarty P. L. Biotransformation of tetrachloroethylene to trichloroethylene, dichloroethylene, vinyl chloride, and carbon dioxide under methanogenic conditions. Appl Environ Microbiol. 1985 May;49(5):1080–1083. doi: 10.1128/aem.49.5.1080-1083.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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