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. 1990 Nov;56(11):3593–3594. doi: 10.1128/aem.56.11.3593-3594.1990

Specific plate assay for bacterial heparinase.

J J Zimmermann 1, R Langer 1, C L Cooney 1
PMCID: PMC185029  PMID: 2268165

Abstract

A procedure was developed for detecting heparinase activity on heparin agar plates. The method is based on the differential precipitation of heparin and heparinase-generated heparin fragments by protamine sulfate. Heparinase activity is detected by the presence of clear zones against a white background. This method can be used to screen for the expression of recombinant heparinase and to identify Flavobacterium heparinum mutants expressing heparinase constitutively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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