Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1983 Dec;24(6):925–929. doi: 10.1128/aac.24.6.925

Properties of novel beta-lactamase produced by Bacteroides fragilis.

A Yotsuji, S Minami, M Inoue, S Mitsuhashi
PMCID: PMC185409  PMID: 6607033

Abstract

Bacteroides fragilis strains were isolated from clinical specimens. B. fragilis G-237 was highly resistant to beta-lactam antibiotics due to beta-lactamase production. The purified enzyme from this strain gave a single protein band on polyacrylamide gel electrophoresis. The isoelectric point was 4.8, and the molecular weight was estimated to be 26,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme activity was inhibited by p-chloromercuribenzoate and iodine but not by clavulanic acid or sulbactam. The purified enzyme showed a unique substrate profile by hydrolyzing at a high rate most of the cephalosporins, including cephamycin derivatives, penicillins, and imipenem (formerly imipemide, N-formimidoyl thienamycin, or MK 0787).

Full text

PDF
929

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. D., Sykes R. B. Characterisation of a -lactamase obtained from a strain of Bacteroides fragilis resistant to -lactam antibiotics. J Med Microbiol. 1973 May;6(2):201–206. doi: 10.1099/00222615-6-2-201. [DOI] [PubMed] [Google Scholar]
  2. Kuwabara S. Purification and properties of two extracellular beta-lactamases from Bacillus cereus 569-H. Biochem J. 1970 Jul;118(3):457–465. doi: 10.1042/bj1180457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  4. Minami S., Yotsuji A., Inoue M., Mitsuhashi S. Induction of beta-lactamase by various beta-lactam antibiotics in Enterobacter cloacae. Antimicrob Agents Chemother. 1980 Sep;18(3):382–385. doi: 10.1128/aac.18.3.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Murray P. R., Rosenblatt J. E. Penicillin resistance and penicillinase production in clinical isolates of Bacteroides melaninogenicus. Antimicrob Agents Chemother. 1977 Apr;11(4):605–608. doi: 10.1128/aac.11.4.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Novick R. P., Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. doi: 10.1111/j.1749-6632.1971.tb30664.x. [DOI] [PubMed] [Google Scholar]
  7. Olsson-Liljequist B., Dornbusch K., Nord C. E. Characterization of three different beta-lactamases from the Bacteroides fragilis group. Antimicrob Agents Chemother. 1980 Aug;18(2):220–225. doi: 10.1128/aac.18.2.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Olsson B., Nord C. E., Wadström T. Formation of beta-lactamase in Bacteroides fragilis: cell-bound and extracellular activity. Antimicrob Agents Chemother. 1976 May;9(5):727–735. doi: 10.1128/aac.9.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pinkus G., Veo G., Braude A. I. Bacteroides penicillinase. J Bacteriol. 1968 Oct;96(4):1437–1438. doi: 10.1128/jb.96.4.1437-1438.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Richmond M. H., Sykes R. B. The beta-lactamases of gram-negative bacteria and their possible physiological role. Adv Microb Physiol. 1973;9:31–88. doi: 10.1016/s0065-2911(08)60376-8. [DOI] [PubMed] [Google Scholar]
  11. Saino Y., Kobayashi F., Inoue M., Mitsuhashi S. Purification and properties of inducible penicillin beta-lactamase isolated from Pseudomonas maltophilia. Antimicrob Agents Chemother. 1982 Oct;22(4):564–570. doi: 10.1128/aac.22.4.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sato K., Inoue M., Mitsuhashi S. Activity of beta-lactamase produced by Bacteroides fragilis against newly introduced cephalosporins. Antimicrob Agents Chemother. 1980 Apr;17(4):736–737. doi: 10.1128/aac.17.4.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sato K., Matsuura Y., Inoue M., Mitsuhashi S. Properties of a new penicillinase type produced by Bacteroides fragilis. Antimicrob Agents Chemother. 1982 Oct;22(4):579–584. doi: 10.1128/aac.22.4.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sato K., Matsuura Y., Miyata K., Inoue M., Mitsuhashi S. Characterization of cephalosporinases from Bacteroides fragilis, Bacteroides thetaiotaomicron and Bacteroides vulgatus. J Antibiot (Tokyo) 1983 Jan;36(1):76–85. doi: 10.7164/antibiotics.36.76. [DOI] [PubMed] [Google Scholar]
  15. Sutter V. L., Finegold S. M. Susceptibility of anaerobic bacteria to 23 antimicrobial agents. Antimicrob Agents Chemother. 1976 Oct;10(4):736–752. doi: 10.1128/aac.10.4.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sykes R. B., Matthew M. The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics. J Antimicrob Chemother. 1976 Jun;2(2):115–157. doi: 10.1093/jac/2.2.115. [DOI] [PubMed] [Google Scholar]
  17. Tai M., Fukuoka Y., Yotsuji A., Kumano K., Takahata M., Mikami H., Yasuda T., Saikawa I., Mitsuhashi S. In vitro and in vivo antibacterial activity of T-1982, a new semisynthetic cephamycin antibiotic. Antimicrob Agents Chemother. 1982 Nov;22(5):728–734. doi: 10.1128/aac.22.5.728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Toda M., Sato K., Nakazawa H., Inoue M., Mitsuhashi S. Effect of N-formimidoyl thienamycin (MK0787) on beta-lactamases and activity against beta-lactamase-producing strains. Antimicrob Agents Chemother. 1980 Nov;18(5):837–838. doi: 10.1128/aac.18.5.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yaginuma S., Terakado N., Mitsuhashi S. Biochemical properties of a penicillin beta-lactamase mediated by R factor from Bordetella bronchiseptica. Antimicrob Agents Chemother. 1975 Sep;8(3):238–242. doi: 10.1128/aac.8.3.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yoshida T., Matsuura S., Mayama M., Kameda Y., Kuwahara S. Moxalactam (6059-S), a novel 1-oxa-beta-lactam with an expanded antibacterial spectrum: laboratory evaluation. Antimicrob Agents Chemother. 1980 Mar;17(3):302–312. doi: 10.1128/aac.17.3.302. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES