Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1988 Sep;95(1):300–308. doi: 10.1111/j.1476-5381.1988.tb16577.x

Evidence that the histamine sensitivity and responsiveness of guinea-pig isolated trachea are modulated by epithelial prostaglandin E2 production.

G Braunstein 1, C Labat 1, S Brunelleschi 1, J Benveniste 1, J Marsac 1, C Brink 1
PMCID: PMC1854118  PMID: 3146396

Abstract

1. Guinea-pig isolated tracheal preparations in which the epithelium had been removed exhibited a greater contractile response to histamine (intact: 1.91 +/- 0.12 g; n = 6 and rubbed: 2.76 +/- 0.15 g; n = 11; P less than 0.001). The histamine sensitivity (pD2 value) of these preparations was also significantly greater (intact: 4.80 +/- 0.04 and rubbed: 5.40 +/- 0.08; P less than 0.01). 2. Indomethacin suppressed the basal tone of both intact and rubbed preparations but was more effective in the former tissues (intact: -0.70 +/- 0.14 g; n = 22 and rubbed: -0.17 +/- 0.05 g; n = 12; P less than 0.02). 3. Arachidonic acid (AA; 10 microM) suppressed the basal tone of intact tissues but contracted such preparations following indomethacin treatment (1.7 microM; 30 min). However, in rubbed tissues AA (10 microM) induced a contraction which was attenuated following indomethacin treatment. 4. Prostaglandin E2 (PGE2; 0.01 and 0.1 microM) suppressed the basal tone of intact preparations and always evoked contraction of rubbed tissues. Following indomethacin treatment PGE2 (0.01 and 0.1 microM) generally evoked spasm of intact and rubbed tissues while at higher concentrations (1 microM) relaxant effects were observed. 5. Removal of the epithelium did not alter the relaxant effect of PGE2 (pD2 value) on histamine (50 microM)-contracted tissues (intact: 6.86 +/- 0.08 and rubbed: 7.10 +/- 0.3; n = 4; P greater than 0.1). 6. In rubbed preparations treated with indomethacin, PGE2 (0.01 and 0.1 microM) evoked spasm. However, when added to preparations contracted with 5 microM histamine, PGE2 always caused relaxation.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
303

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adcock J. J., Garland L. G. A possible role for lipoxygenase products as regulators of airway smooth muscle reactivity. Br J Pharmacol. 1980 Jun;69(2):167–169. doi: 10.1111/j.1476-5381.1980.tb07886.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnes P. J., Cuss F. M., Palmer J. B. The effect of airway epithelium on smooth muscle contractility in bovine trachea. Br J Pharmacol. 1985 Nov;86(3):685–691. doi: 10.1111/j.1476-5381.1985.tb08946.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Butler G. B., Adler K. B., Evans J. N., Morgan D. W., Szarek J. L. Modulation of rabbit airway smooth muscle responsiveness by respiratory epithelium. Involvement of an inhibitory metabolite of arachidonic acid. Am Rev Respir Dis. 1987 May;135(5):1099–1104. doi: 10.1164/arrd.1987.135.5.1099. [DOI] [PubMed] [Google Scholar]
  4. Coleman R. A., Kennedy I. Characterisation of the prostanoid receptors mediating contraction of guinea-pig isolated trachea. Prostaglandins. 1985 Mar;29(3):363–375. doi: 10.1016/0090-6980(85)90096-6. [DOI] [PubMed] [Google Scholar]
  5. Farmer S. G., Fedan J. S., Hay D. W., Raeburn D. The effects of epithelium removal on the sensitivity of guinea-pig isolated trachealis to bronchodilator drugs. Br J Pharmacol. 1986 Oct;89(2):407–414. doi: 10.1111/j.1476-5381.1986.tb10274.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farmer S. G., Hay D. W., Raeburn D., Fedan J. S. Relaxation of guinea-pig tracheal smooth muscle to arachidonate is converted to contraction following epithelium removal. Br J Pharmacol. 1987 Sep;92(1):231–236. doi: 10.1111/j.1476-5381.1987.tb11316.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Flavahan N. A., Aarhus L. L., Rimele T. J., Vanhoutte P. M. Respiratory epithelium inhibits bronchial smooth muscle tone. J Appl Physiol (1985) 1985 Mar;58(3):834–838. doi: 10.1152/jappl.1985.58.3.834. [DOI] [PubMed] [Google Scholar]
  8. Gardiner P. J. Characterization of prostanoid relaxant/inhibitory receptors (psi) using a highly selective agonist, TR4979. Br J Pharmacol. 1986 Jan;87(1):45–56. doi: 10.1111/j.1476-5381.1986.tb10155.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gardiner P. J., Collier H. O. Specific receptors for prostaglandins in airways. Prostaglandins. 1980 Jun;19(6):819–841. doi: 10.1016/0090-6980(80)90116-1. [DOI] [PubMed] [Google Scholar]
  10. Goldie R. G., Papadimitriou J. M., Paterson J. W., Rigby P. J., Self H. M., Spina D. Influence of the epithelium on responsiveness of guinea-pig isolated trachea to contractile and relaxant agonists. Br J Pharmacol. 1986 Jan;87(1):5–14. doi: 10.1111/j.1476-5381.1986.tb10150.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grodzinska L., Panczenko B., Gryglewski R. J. Generation of prostaglandin E-like material by the guinea-pig trachea contracted by histamine. J Pharm Pharmacol. 1975 Feb;27(2):88–91. doi: 10.1111/j.2042-7158.1975.tb09414.x. [DOI] [PubMed] [Google Scholar]
  12. Hay D. W., Farmer S. G., Raeburn D., Robinson V. A., Fleming W. W., Fedan J. S. Airway epithelium modulates the reactivity of guinea-pig respiratory smooth muscle. Eur J Pharmacol. 1986 Sep 23;129(1-2):11–18. doi: 10.1016/0014-2999(86)90330-4. [DOI] [PubMed] [Google Scholar]
  13. Haye-Legrand I., Cerrina J., Raffestin B., Labat C., Boullet C., Bayol A., Benveniste J., Brink C. Histamine contraction of isolated human airway muscle preparations: role of prostaglandins. J Pharmacol Exp Ther. 1986 Nov;239(2):536–541. [PubMed] [Google Scholar]
  14. Holroyde M. C. The influence of epithelium on the responsiveness of guinea-pig isolated trachea. Br J Pharmacol. 1986 Mar;87(3):501–507. doi: 10.1111/j.1476-5381.1986.tb10192.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ilhan M., Sahin I. Tracheal epithelium releases a vascular smooth muscle relaxant factor: demonstration by bioassay. Eur J Pharmacol. 1986 Nov 19;131(2-3):293–296. doi: 10.1016/0014-2999(86)90586-8. [DOI] [PubMed] [Google Scholar]
  16. Mitchell H. W., Denborough M. A. The metabolism of arachidonic acid in the isolated tracheal and lung strip preparations of guinea-pigs. Lung. 1980;158(3):121–129. doi: 10.1007/BF02713714. [DOI] [PubMed] [Google Scholar]
  17. Nijkamp F. P., Folkerts G. Reversal of arachidonic acid-induced guinea-pig tracheal relaxation into contraction after epithelium removal. Eur J Pharmacol. 1986 Nov 19;131(2-3):315–316. doi: 10.1016/0014-2999(86)90591-1. [DOI] [PubMed] [Google Scholar]
  18. Orehek J., Douglas J. S., Bouhuys A. Contractile responses of the guinea-pig trachea in vitro: modification by prostaglandin synthesis-inhibiting drugs. J Pharmacol Exp Ther. 1975 Sep;194(3):554–564. [PubMed] [Google Scholar]
  19. Orehek J., Douglas J. S., Lewis A. J., Bouhuys A. Prostaglandin regulation of airway smooth muscle tone. Nat New Biol. 1973 Sep 19;245(142):84–85. doi: 10.1038/newbio245084a0. [DOI] [PubMed] [Google Scholar]
  20. Tschirhart E., Frossard N., Bertrand C., Landry Y. Arachidonic acid metabolites and airway epithelium-dependent relaxant factor. J Pharmacol Exp Ther. 1987 Oct;243(1):310–316. [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES