Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1989 May;97(1):133–138. doi: 10.1111/j.1476-5381.1989.tb11933.x

Evidence for a dihydropyridine-sensitive and conotoxin-insensitive release of noradrenaline and uptake of calcium in adrenal chromaffin cells.

P J Owen 1, D B Marriott 1, M R Boarder 1
PMCID: PMC1854474  PMID: 2470457

Abstract

1. It has been suggested that neuronal voltage-sensitive calcium channels (VSCC) may be divided into dihydropyridine (DHP)-sensitive (L) and DHP-insensitive (N and T), and that both the L and the N type channels are attenuated by the peptide blocker omega-conotoxin. Here the effects of omega-conotoxin on release of noradrenaline and uptake of calcium in bovine adrenal chromaffin cells were investigated. 2. Release of noradrenaline in response to 25 mM K+, 65 mM K+, 10 nM bradykinin or 10 microM prostaglandin E1 was not affected by omega-conotoxin in the range 10 nM-1 microM. 3. 45Ca2+ uptake stimulated by high K+ and prostaglandin was attenuated by 1 microM nitrendipine and enhanced by 1 microM Bay K 8644; these calcium fluxes were not modified by 20 nM omega-conotoxin. 4. With superfused rat brain striatal slices in the same medium as the above cell studies, release of dopamine in response to 25 mM K+ was attenuated by 20 nM omega-conotoxin. 5. These results show that in these neurone-like cells, release may be effected by calcium influx through DHP-sensitive but omega-conotoxin-insensitive VSCC, a result inconsistent with the suggestion that omega-conotoxin blocks both L-type and N-type neuronal calcium channels.

Full text

PDF
134

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson A. J., Harvey A. L. Omega-conotoxin does not block the verapamil-sensitive calcium channels at mouse motor nerve terminals. Neurosci Lett. 1987 Nov 23;82(2):177–180. doi: 10.1016/0304-3940(87)90125-x. [DOI] [PubMed] [Google Scholar]
  2. Barhanin J., Schmid A., Lazdunski M. Properties of structure and interaction of the receptor for omega-conotoxin, a polypeptide active on Ca2+ channels. Biochem Biophys Res Commun. 1988 Feb 15;150(3):1051–1062. doi: 10.1016/0006-291x(88)90736-x. [DOI] [PubMed] [Google Scholar]
  3. Boarder M. R., Marriott D., Adams M. Stimulus secretion coupling in cultured chromaffin cells. Dependency on external sodium and on dihydropyridine-sensitive calcium channels. Biochem Pharmacol. 1987 Jan 1;36(1):163–167. doi: 10.1016/0006-2952(87)90394-7. [DOI] [PubMed] [Google Scholar]
  4. Cruz L. J., Johnson D. S., Olivera B. M. Characterization of the omega-conotoxin target. Evidence for tissue-specific heterogeneity in calcium channel types. Biochemistry. 1987 Feb 10;26(3):820–824. doi: 10.1021/bi00377a024. [DOI] [PubMed] [Google Scholar]
  5. Dooley D. J., Lupp A., Hertting G. Inhibition of central neurotransmitter release by omega-conotoxin GVIA, a peptide modulator of the N-type voltage-sensitive calcium channel. Naunyn Schmiedebergs Arch Pharmacol. 1987 Oct;336(4):467–470. doi: 10.1007/BF00164885. [DOI] [PubMed] [Google Scholar]
  6. Fonteriz R. I., Gandia L., Lopez M. G., Artalejo C. R., García A. G. Dihydropyridine chirality at the chromaffin cell calcium channel. Brain Res. 1987 Apr 7;408(1-2):359–362. doi: 10.1016/0006-8993(87)90405-7. [DOI] [PubMed] [Google Scholar]
  7. Holz G. G., 4th, Dunlap K., Kream R. M. Characterization of the electrically evoked release of substance P from dorsal root ganglion neurons: methods and dihydropyridine sensitivity. J Neurosci. 1988 Feb;8(2):463–471. doi: 10.1523/JNEUROSCI.08-02-00463.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kerr L. M., Yoshikami D. A venom peptide with a novel presynaptic blocking action. Nature. 1984 Mar 15;308(5956):282–284. doi: 10.1038/308282a0. [DOI] [PubMed] [Google Scholar]
  9. Koyama Y., Kitayama S., Dohi T., Tsujimoto A. Evidence that prostaglandins activate calcium channels to enhance basal and stimulation-evoked catecholamine release from bovine adrenal chromaffin cells in culture. Biochem Pharmacol. 1988 May 1;37(9):1725–1730. doi: 10.1016/0006-2952(88)90435-2. [DOI] [PubMed] [Google Scholar]
  10. Maggi C. A., Patacchini R., Santicioli P., Lippe I. T., Giuliani S., Geppetti P., Del Bianco E., Selleri S., Meli A. The effect of omega conotoxin GVIA, a peptide modulator of the N-type voltage sensitive calcium channels, on motor responses produced by activation of efferent and sensory nerves in mammalian smooth muscle. Naunyn Schmiedebergs Arch Pharmacol. 1988 Aug;338(2):107–113. doi: 10.1007/BF00174856. [DOI] [PubMed] [Google Scholar]
  11. Marriott D., Adams M., Boarder M. R. Effect of forskolin and prostaglandin E1 on stimulus secretion coupling in cultured bovine adrenal chromaffin cells. J Neurochem. 1988 Feb;50(2):616–623. doi: 10.1111/j.1471-4159.1988.tb02955.x. [DOI] [PubMed] [Google Scholar]
  12. McCleskey E. W., Fox A. P., Feldman D. H., Cruz L. J., Olivera B. M., Tsien R. W., Yoshikami D. Omega-conotoxin: direct and persistent blockade of specific types of calcium channels in neurons but not muscle. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4327–4331. doi: 10.1073/pnas.84.12.4327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mefford I. N. Application of high performance liquid chromatography with electrochemical detection to neurochemical analysis: measurement of catecholamines, serotonin and metabolites in rat brain. J Neurosci Methods. 1981 Feb;3(3):207–224. doi: 10.1016/0165-0270(81)90056-x. [DOI] [PubMed] [Google Scholar]
  14. Miller R. J. Multiple calcium channels and neuronal function. Science. 1987 Jan 2;235(4784):46–52. doi: 10.1126/science.2432656. [DOI] [PubMed] [Google Scholar]
  15. Nachshen D. A., Blaustein M. P. The effects of some organic "calcium antagonists" on calcium influx in presynaptic nerve terminals. Mol Pharmacol. 1979 Sep;16(2):576–586. [PubMed] [Google Scholar]
  16. Nowycky M. C., Fox A. P., Tsien R. W. Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 1985 Aug 1;316(6027):440–443. doi: 10.1038/316440a0. [DOI] [PubMed] [Google Scholar]
  17. Olivera B. M., McIntosh J. M., Cruz L. J., Luque F. A., Gray W. R. Purification and sequence of a presynaptic peptide toxin from Conus geographus venom. Biochemistry. 1984 Oct 23;23(22):5087–5090. doi: 10.1021/bi00317a001. [DOI] [PubMed] [Google Scholar]
  18. Perney T. M., Hirning L. D., Leeman S. E., Miller R. J. Multiple calcium channels mediate neurotransmitter release from peripheral neurons. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6656–6659. doi: 10.1073/pnas.83.17.6656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rampe D., Janis R. A., Triggle D. J. BAY K 8644, a 1,4-dihydropyridine Ca2+ channel activator: dissociation of binding and functional effects in brain synaptosomes. J Neurochem. 1984 Dec;43(6):1688–1692. doi: 10.1111/j.1471-4159.1984.tb06096.x. [DOI] [PubMed] [Google Scholar]
  20. Rane S. G., Holz G. G., 4th, Dunlap K. Dihydropyridine inhibition of neuronal calcium current and substance P release. Pflugers Arch. 1987 Aug;409(4-5):361–366. doi: 10.1007/BF00583789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reynolds I. J., Wagner J. A., Snyder S. H., Thayer S. A., Olivera B. M., Miller R. J. Brain voltage-sensitive calcium channel subtypes differentiated by omega-conotoxin fraction GVIA. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8804–8807. doi: 10.1073/pnas.83.22.8804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rivier J., Galyean R., Gray W. R., Azimi-Zonooz A., McIntosh J. M., Cruz L. J., Olivera B. M. Neuronal calcium channel inhibitors. Synthesis of omega-conotoxin GVIA and effects on 45Ca uptake by synaptosomes. J Biol Chem. 1987 Jan 25;262(3):1194–1198. [PubMed] [Google Scholar]
  23. Sano K., Enomoto K., Maeno T. Effects of synthetic omega-conotoxin, a new type Ca2+ antagonist, on frog and mouse neuromuscular transmission. Eur J Pharmacol. 1987 Sep 11;141(2):235–241. doi: 10.1016/0014-2999(87)90268-8. [DOI] [PubMed] [Google Scholar]
  24. Sher E., Pandiella A., Clementi F. Omega-conotoxin binding and effects on calcium channel function in human neuroblastoma and rat pheochromocytoma cell lines. FEBS Lett. 1988 Aug 1;235(1-2):178–182. doi: 10.1016/0014-5793(88)81258-4. [DOI] [PubMed] [Google Scholar]
  25. Turner T. J., Goldin S. M. Calcium channels in rat brain synaptosomes: identification and pharmacological characterization. High affinity blockade by organic Ca2+ channel blockers. J Neurosci. 1985 Mar;5(3):841–849. doi: 10.1523/JNEUROSCI.05-03-00841.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Waymire J. C., Bennett W. F., Boehme R., Hankins L., Gilmer-Waymire K., Haycock J. W. Bovine adrenal chromaffin cells: high-yield purification and viability in suspension culture. J Neurosci Methods. 1983 Apr;7(4):329–351. doi: 10.1016/0165-0270(83)90026-2. [DOI] [PubMed] [Google Scholar]
  27. Yeager R. E., Yoshikami D., Rivier J., Cruz L. J., Miljanich G. P. Transmitter release from presynaptic terminals of electric organ: inhibition by the calcium channel antagonist omega Conus toxin. J Neurosci. 1987 Aug;7(8):2390–2396. [PMC free article] [PubMed] [Google Scholar]
  28. el-Din M. M., Malik K. U. Differential effect of omega-conotoxin on release of the adrenergic transmitter and the vasoconstrictor response to noradrenaline in the rat isolated kidney. Br J Pharmacol. 1988 Jun;94(2):355–362. doi: 10.1111/j.1476-5381.1988.tb11537.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES