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. 1982 Dec;22(6):1051–1057. doi: 10.1128/aac.22.6.1051

Lysis of Trypanosoma cruzi by Pseudomonas fluorescens.

T I Mercado, A Colón-Whitt
PMCID: PMC185719  PMID: 6818898

Abstract

Trypomastigotes of Trypanosoma cruzi isolated from the blood of infected mice were lysed within 24 h by an extracellular substance produced by Pseudomonas fluorescens. Isolation of the anti-trypanosomal factor (ATF) was accomplished by growth of the organisms in a defined medium, extracellular secretion by the sedimented cells, sterilization by filtration, lyophilization, dialysis, and gel filtration. Chromatographic separation with Sephadex G-25 and G-200 disclosed the occurrence of three active fractions. ATF-I(1) exhibited a molecular weight higher than 440,000. ATF-II and ATF-III were considerably smaller (molecular weights approximately 1,355 and 1,060, respectively). The lytic substance contained protein and lipopolysaccharide, was resistant to heat and freezing, was not proteolytic or hemolytic, and was not inhibited by trypsin but was suppressed by pronase.

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Selected References

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  1. ALFORD J. A., PIERCE D. A. PRODUCTION OF LIPASE BY PSEUDOMONAS FRAGI IN A SYNTHETIC MEDIUM. J Bacteriol. 1963 Jul;86:24–29. doi: 10.1128/jb.86.1.24-29.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Callahan L. T., 3rd Pseudomonas aeruginosa exotoxin: purification by preparative polyacrylamide gel electrophoresis and the development of a highly specific antitoxin serum. Infect Immun. 1976 Jul;14(1):55–61. doi: 10.1128/iai.14.1.55-61.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cáceres O., Salgueiro M. C., Kimura E., Moraes G., Machado T. A. Aço do Aspergillus sp. sobre o Trypanosoma cruzi "in vitro". Rev Inst Med Trop Sao Paulo. 1979 Jan-Feb;21(1):5–12. [PubMed] [Google Scholar]
  4. Doi O., Nojima S. Phospholipase C from Pseudomonas fluorescens. Biochim Biophys Acta. 1971 Nov 5;248(2):234–244. doi: 10.1016/0005-2760(71)90011-7. [DOI] [PubMed] [Google Scholar]
  5. GRAEF W., BICKEL H. [Antibiotic properties and toxicity of a substance isolated from Pseudomonas fluorescens]. Arch Hyg Bakteriol. 1961 Feb;145:21–31. [PubMed] [Google Scholar]
  6. Hochstein H. D., Elfin R. J., Cooper J. F., Seligmann E. B., Jr, Wolff S. M. Further developments of Limulus Amebocyte Lysate test. Bull Parenter Drug Assoc. 1973 May-Jun;27(3):139–148. [PubMed] [Google Scholar]
  7. Kiprianova E. A., Smirnov V. V. Pseudomonas fluorescens--produtsent antibioticheskikh veshchestv. Antibiotiki. 1981 Feb;26(2):135–143. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Leisinger T., Margraff R. Secondary metabolites of the fluorescent pseudomonads. Microbiol Rev. 1979 Sep;43(3):422–442. doi: 10.1128/mr.43.3.422-442.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mayerhofer H. J., Marshall R. T., White C. H., Lu M. Characterization of a heat-stable protease of Pseudomonas fluorescens P26. Appl Microbiol. 1973 Jan;25(1):44–48. doi: 10.1128/am.25.1.44-48.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meinke G., Berk R. S. In vivo studies with a toxic fraction of Pseudomonas aeruginosa. Proc Soc Exp Biol Med. 1970 Nov;135(2):360–363. doi: 10.3181/00379727-135-35051. [DOI] [PubMed] [Google Scholar]
  12. Mencher J. R., Ng H., Alford J. A. The extracellular nature of the lipase of Pseudomonas fragi. Biochim Biophys Acta. 1965 Dec 2;106(3):628–630. doi: 10.1016/0005-2760(65)90079-2. [DOI] [PubMed] [Google Scholar]
  13. Mercado T. I., Katusha K. Isolation of Trypanosoma cruzi from the blood of infected mice by column chromatography. Prep Biochem. 1979;9(1):97–106. doi: 10.1080/00327487908061675. [DOI] [PubMed] [Google Scholar]
  14. Mercado T. I. Trypanosoma cruzi: lactate dehydrogenase isoenzymes and infections in mice. Exp Parasitol. 1976 Dec;40(3):411–420. doi: 10.1016/0014-4894(76)90108-9. [DOI] [PubMed] [Google Scholar]
  15. PINHEIRO A. J., LISKA B. J., PARMELEE C. E. HEAT STABILITY OF LIPASES OF SELECTED PSYCHROPHILIC BACTERIA IN MILK AND PURDUE SWISS-TYPE CHEESE. J Dairy Sci. 1965 Jul;48:983–984. doi: 10.3168/jds.s0022-0302(65)88372-2. [DOI] [PubMed] [Google Scholar]
  16. Rastogi S. C., Seligmann E. B., Jr, Hochstein H. D., Dawson J. H., Farag L. G., Marquina R. E. Statistical procedure for evaluating the sensitivity of Limulus amoebocyte lysate by using a reference lysate. Appl Environ Microbiol. 1979 Nov;38(5):911–915. doi: 10.1128/aem.38.5.911-915.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reynolds M. T., Falkiner F. R., Hardy R., Keane C. T. Differentiation of fluorescent pseudomonads by their effect on milk agar. J Med Microbiol. 1979 Aug;12(3):379–382. doi: 10.1099/00222615-12-3-379. [DOI] [PubMed] [Google Scholar]
  18. Severina L. O., Bashkatova N. A. Vydelenie i svoistva lipazy nz Pseudomon as fluorescens 533-5b. Biokhimiia. 1979 Jan;44(1):116–124. [PubMed] [Google Scholar]
  19. Speck M. L., Adams D. M. Heat resistant proteolytic enzymes from bacterial sources. J Dairy Sci. 1976 Apr;59(4):786–789. doi: 10.3168/jds.S0022-0302(76)84276-2. [DOI] [PubMed] [Google Scholar]
  20. Stinson M. W., Hayden C. Secretion of phospholipase C by Pseudomonas aeruginosa. Infect Immun. 1979 Aug;25(2):558–564. doi: 10.1128/iai.25.2.558-564.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Villalta F., de Souza W., Leon W. The effect of lampit on Trypanosoma cruzi in mice organs and in the bloodstream. Z Parasitenkd. 1979;61(1):21–27. doi: 10.1007/BF00927084. [DOI] [PubMed] [Google Scholar]
  22. Whiteside T. L., Corpe W. A. Extracellular enzymes produced by a Pseudomonas sp. and their effect on cell envelopes of Chromobacterium violaceum. Can J Microbiol. 1969 Jan;15(1):81–92. doi: 10.1139/m69-013. [DOI] [PubMed] [Google Scholar]
  23. ZYSKIND J. W., PATTEE P. A., LACHE M. STAPHYLOLYTIC SUBSTANCE FROM A SPECIES OF PSEUDOMONAS. Science. 1965 Mar 19;147(3664):1458–1459. doi: 10.1126/science.147.3664.1458. [DOI] [PubMed] [Google Scholar]

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