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. 1982 Dec;22(6):1051–1057. doi: 10.1128/aac.22.6.1051

Lysis of Trypanosoma cruzi by Pseudomonas fluorescens.

T I Mercado, A Colón-Whitt
PMCID: PMC185719  PMID: 6818898

Abstract

Trypomastigotes of Trypanosoma cruzi isolated from the blood of infected mice were lysed within 24 h by an extracellular substance produced by Pseudomonas fluorescens. Isolation of the anti-trypanosomal factor (ATF) was accomplished by growth of the organisms in a defined medium, extracellular secretion by the sedimented cells, sterilization by filtration, lyophilization, dialysis, and gel filtration. Chromatographic separation with Sephadex G-25 and G-200 disclosed the occurrence of three active fractions. ATF-I(1) exhibited a molecular weight higher than 440,000. ATF-II and ATF-III were considerably smaller (molecular weights approximately 1,355 and 1,060, respectively). The lytic substance contained protein and lipopolysaccharide, was resistant to heat and freezing, was not proteolytic or hemolytic, and was not inhibited by trypsin but was suppressed by pronase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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