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. 1997 Dec;151(6):1785–1790.

Chronic obstructive pulmonary disease: role of bronchiolar mast cells and macrophages.

W F Grashoff 1, J K Sont 1, P J Sterk 1, P S Hiemstra 1, W I de Boer 1, J Stolk 1, J Han 1, J M van Krieken 1
PMCID: PMC1858350  PMID: 9403729

Abstract

Chronic obstructive pulmonary disease (COPD) is considered to be caused in part by smoking-induced inflammation, but it is unknown which inflammatory cells within the small airways are associated with the obstruction. We investigated the inflammatory infiltrate in the small airways of 16 current or ex-smokers with COPD (FEV1 < or = 75% predicted) and 15 without COPD (FEV1 > or = 85% predicted) in pneumectomy specimens that were removed for lung cancer. Mast cells, macrophages, neutrophils, eosinophils, T cells, and B cells were identified using immunohistochemistry on formalin-fixed, paraffin-embedded specimens. These cells were quantified in the epithelium and the remainder of the airway wall. The number of mast cells and macrophages in the epithelium, but not in the remainder of the airway wall, was significantly increased in patients with COPD. Neutrophil and T cell numbers did not differ between the groups. Only few B cells and eosinophils were present in both groups. Smoking history, perioperative steroid usage, tumor localization, or reversibility in the FEV1 to salbutamol could not account for the observed differences. We conclude that the number of epithelial mast cells and macrophages is increased in the bronchioli in smokers with airflow limitation, suggesting a role in development of COPD.

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Selected References

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  1. Bosken C. H., Hards J., Gatter K., Hogg J. C. Characterization of the inflammatory reaction in the peripheral airways of cigarette smokers using immunocytochemistry. Am Rev Respir Dis. 1992 Apr;145(4 Pt 1):911–917. doi: 10.1164/ajrccm/145.4_Pt_1.911. [DOI] [PubMed] [Google Scholar]
  2. Bradding P., Roberts J. A., Britten K. M., Montefort S., Djukanovic R., Mueller R., Heusser C. H., Howarth P. H., Holgate S. T. Interleukin-4, -5, and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines. Am J Respir Cell Mol Biol. 1994 May;10(5):471–480. doi: 10.1165/ajrcmb.10.5.8179909. [DOI] [PubMed] [Google Scholar]
  3. Cosio M., Ghezzo H., Hogg J. C., Corbin R., Loveland M., Dosman J., Macklem P. T. The relations between structural changes in small airways and pulmonary-function tests. N Engl J Med. 1978 Jun 8;298(23):1277–1281. doi: 10.1056/NEJM197806082982303. [DOI] [PubMed] [Google Scholar]
  4. Di Stefano A., Turato G., Maestrelli P., Mapp C. E., Ruggieri M. P., Roggeri A., Boschetto P., Fabbri L. M., Saetta M. Airflow limitation in chronic bronchitis is associated with T-lymphocyte and macrophage infiltration of the bronchial mucosa. Am J Respir Crit Care Med. 1996 Feb;153(2):629–632. doi: 10.1164/ajrccm.153.2.8564109. [DOI] [PubMed] [Google Scholar]
  5. Djukanović R., Roche W. R., Wilson J. W., Beasley C. R., Twentyman O. P., Howarth R. H., Holgate S. T. Mucosal inflammation in asthma. Am Rev Respir Dis. 1990 Aug;142(2):434–457. doi: 10.1164/ajrccm/142.2.434. [DOI] [PubMed] [Google Scholar]
  6. Eidelman D., Saetta M. P., Ghezzo H., Wang N. S., Hoidal J. R., King M., Cosio M. G. Cellularity of the alveolar walls in smokers and its relation to alveolar destruction. Functional implications. Am Rev Respir Dis. 1990 Jun;141(6):1547–1552. doi: 10.1164/ajrccm/141.6.1547. [DOI] [PubMed] [Google Scholar]
  7. Finkelstein R., Fraser R. S., Ghezzo H., Cosio M. G. Alveolar inflammation and its relation to emphysema in smokers. Am J Respir Crit Care Med. 1995 Nov;152(5 Pt 1):1666–1672. doi: 10.1164/ajrccm.152.5.7582312. [DOI] [PubMed] [Google Scholar]
  8. Fletcher C., Peto R. The natural history of chronic airflow obstruction. Br Med J. 1977 Jun 25;1(6077):1645–1648. doi: 10.1136/bmj.1.6077.1645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fournier M., Lebargy F., Le Roy Ladurie F., Lenormand E., Pariente R. Intraepithelial T-lymphocyte subsets in the airways of normal subjects and of patients with chronic bronchitis. Am Rev Respir Dis. 1989 Sep;140(3):737–742. doi: 10.1164/ajrccm/140.3.737. [DOI] [PubMed] [Google Scholar]
  10. Hobson J. E., Wright J. L., Wiggs B. R., Hogg J. C. Comparison of the cell content of lung lavage fluid with the presence of emphysema and peripheral airways inflammation in resected lungs. Respiration. 1986;50(1):1–8. doi: 10.1159/000194900. [DOI] [PubMed] [Google Scholar]
  11. Hogg J. C., Macklem P. T., Thurlbeck W. M. Site and nature of airway obstruction in chronic obstructive lung disease. N Engl J Med. 1968 Jun 20;278(25):1355–1360. doi: 10.1056/NEJM196806202782501. [DOI] [PubMed] [Google Scholar]
  12. Hunninghake G. W., Crystal R. G. Cigarette smoking and lung destruction. Accumulation of neutrophils in the lungs of cigarette smokers. Am Rev Respir Dis. 1983 Nov;128(5):833–838. doi: 10.1164/arrd.1983.128.5.833. [DOI] [PubMed] [Google Scholar]
  13. Kalenderian R., Raju L., Roth W., Schwartz L. B., Gruber B., Janoff A. Elevated histamine and tryptase levels in smokers' bronchoalveolar lavage fluid. Do lung mast cells contribute to smokers' emphysema? Chest. 1988 Jul;94(1):119–123. doi: 10.1378/chest.94.1.119. [DOI] [PubMed] [Google Scholar]
  14. Lacoste J. Y., Bousquet J., Chanez P., Van Vyve T., Simony-Lafontaine J., Lequeu N., Vic P., Enander I., Godard P., Michel F. B. Eosinophilic and neutrophilic inflammation in asthma, chronic bronchitis, and chronic obstructive pulmonary disease. J Allergy Clin Immunol. 1993 Oct;92(4):537–548. doi: 10.1016/0091-6749(93)90078-t. [DOI] [PubMed] [Google Scholar]
  15. Lamb D., Lumsden A. Intra-epithelial mast cells in human airway epithelium: evidence for smoking-induced changes in their frequency. Thorax. 1982 May;37(5):334–342. doi: 10.1136/thx.37.5.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Linden M., Rasmussen J. B., Piitulainen E., Tunek A., Larson M., Tegner H., Venge P., Laitinen L. A., Brattsand R. Airway inflammation in smokers with nonobstructive and obstructive chronic bronchitis. Am Rev Respir Dis. 1993 Nov;148(5):1226–1232. doi: 10.1164/ajrccm/148.5.1226. [DOI] [PubMed] [Google Scholar]
  17. Nagai A., West W. W., Thurlbeck W. M. The National Institutes of Health Intermittent Positive-Pressure Breathing trial: pathology studies. II. Correlation between morphologic findings, clinical findings, and evidence of expiratory air-flow obstruction. Am Rev Respir Dis. 1985 Nov;132(5):946–953. doi: 10.1164/arrd.1985.132.5.946. [DOI] [PubMed] [Google Scholar]
  18. Niewoehner D. E., Kleinerman J., Rice D. B. Pathologic changes in the peripheral airways of young cigarette smokers. N Engl J Med. 1974 Oct 10;291(15):755–758. doi: 10.1056/NEJM197410102911503. [DOI] [PubMed] [Google Scholar]
  19. Ollerenshaw S. L., Woolcock A. J. Characteristics of the inflammation in biopsies from large airways of subjects with asthma and subjects with chronic airflow limitation. Am Rev Respir Dis. 1992 Apr;145(4 Pt 1):922–927. doi: 10.1164/ajrccm/145.4_Pt_1.922. [DOI] [PubMed] [Google Scholar]
  20. Pesci A., Rossi G. A., Bertorelli G., Aufiero A., Zanon P., Olivieri D. Mast cells in the airway lumen and bronchial mucosa of patients with chronic bronchitis. Am J Respir Crit Care Med. 1994 May;149(5):1311–1316. doi: 10.1164/ajrccm.149.5.8173772. [DOI] [PubMed] [Google Scholar]
  21. Saetta M., Di Stefano A., Maestrelli P., Ferraresso A., Drigo R., Potena A., Ciaccia A., Fabbri L. M. Activated T-lymphocytes and macrophages in bronchial mucosa of subjects with chronic bronchitis. Am Rev Respir Dis. 1993 Feb;147(2):301–306. doi: 10.1164/ajrccm/147.2.301. [DOI] [PubMed] [Google Scholar]
  22. Thompson A. B., Daughton D., Robbins R. A., Ghafouri M. A., Oehlerking M., Rennard S. I. Intraluminal airway inflammation in chronic bronchitis. Characterization and correlation with clinical parameters. Am Rev Respir Dis. 1989 Dec;140(6):1527–1537. doi: 10.1164/ajrccm/140.6.1527. [DOI] [PubMed] [Google Scholar]
  23. Thurlbeck W. M. Pathophysiology of chronic obstructive pulmonary disease. Clin Chest Med. 1990 Sep;11(3):389–403. [PubMed] [Google Scholar]
  24. Willems L. N., Kramps J. A., Stijnen T., Sterk P. J., Weening J. J., Dijkman J. H. Antileukoprotease-containing bronchiolar cells. Relationship with morphologic disease of small airways and parenchyma. Am Rev Respir Dis. 1989 May;139(5):1244–1250. doi: 10.1164/ajrccm/139.5.1244. [DOI] [PubMed] [Google Scholar]

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