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. 1994 Feb;62(2):757–758. doi: 10.1128/iai.62.2.757-758.1994

Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis.

J Hill 1, P W Andrew 1, T J Mitchell 1
PMCID: PMC186175  PMID: 8300240

Abstract

The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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