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. 1994 Apr;62(4):1400–1405. doi: 10.1128/iai.62.4.1400-1405.1994

Production of antibodies to antigens of Candida albicans in CBA/H mice.

P J Costantino 1, K M Franklyn 1, N F Gare 1, J R Warmington 1
PMCID: PMC186293  PMID: 7907579

Abstract

Reported targets of the specific immune responses to Candida albicans in human candidiasis include a 47-kDa breakdown product of a 90-kDa heat shock protein (HSP 90) (R. Matthews and J. Burnie, FEMS Microbiol. Lett. 60:25-30, 1989) and the 48-kDa enolase (K.M. Franklyn, J.R. Warmington, A.K. Ott, and R.B. Ashman, Immunol. Cell Biol. 68:173-178, 1990). These proteins are immunodominant antigens of C. albicans. Western blotting (immunoblotting) and immunoprecipitation were used to investigate the humoral response in a mouse model of systemic candidiasis. Resolution of systemic candidiasis in CBA/H mice is associated with a high level of antibody reactivity to C. albicans antigens. A significant antibody response against a non-HSP antigen of 96 kDa which was distinct from the C. albicans HSP 90 antigen was detected. Significant antibody reactivity against an HSP of 75 kDa was also detected. We concluded that resolution of C. albicans infections in CBA/H mice was associated with antibodies to an HSP and a non-HSP of 75 and 96 kDa, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashman R. B., Papadimitriou J. M. Murine candidiasis: strain dependence of host responses after immunization. Immunol Cell Biol. 1988 Jan;66(Pt 3):231–237. doi: 10.1038/icb.1988.29. [DOI] [PubMed] [Google Scholar]
  2. Ashman R. B., Papadimitriou J. M., Ott A. K., Warmington J. R. Antigens and immune responses in Candida albicans infection. Immunol Cell Biol. 1990 Feb;68(Pt 1):1–13. doi: 10.1038/icb.1990.1. [DOI] [PubMed] [Google Scholar]
  3. Brunham R. C., Peeling R., Maclean I., McDowell J., Persson K., Osser S. Postabortal Chlamydia trachomatis salpingitis: correlating risk with antigen-specific serological responses and with neutralization. J Infect Dis. 1987 Apr;155(4):749–755. doi: 10.1093/infdis/155.4.749. [DOI] [PubMed] [Google Scholar]
  4. Danilition S. L., Maclean I. W., Peeling R., Winston S., Brunham R. C. The 75-kilodalton protein of Chlamydia trachomatis: a member of the heat shock protein 70 family? Infect Immun. 1990 Jan;58(1):189–196. doi: 10.1128/iai.58.1.189-196.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  6. Franklyn K. M., Warmington J. R., Ott A. K., Ashman R. B. An immunodominant antigen of Candida albicans shows homology to the enzyme enolase. Immunol Cell Biol. 1990 Jun;68(Pt 3):173–178. doi: 10.1038/icb.1990.24. [DOI] [PubMed] [Google Scholar]
  7. Greenfield R. A. Host defense system interactions with Candida. J Med Vet Mycol. 1992;30(2):89–104. [PubMed] [Google Scholar]
  8. Hector R. F., Domer J. E., Carrow E. W. Immune responses to Candida albicans in genetically distinct mice. Infect Immun. 1982 Dec;38(3):1020–1028. doi: 10.1128/iai.38.3.1020-1028.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lindquist S. The heat-shock response. Annu Rev Biochem. 1986;55:1151–1191. doi: 10.1146/annurev.bi.55.070186.005443. [DOI] [PubMed] [Google Scholar]
  10. Mamula M. J., Janeway C. A., Jr Do B cells drive the diversification of immune responses? Immunol Today. 1993 Apr;14(4):151–154. doi: 10.1016/0167-5699(93)90274-O. [DOI] [PubMed] [Google Scholar]
  11. Matthews R. C., Burnie J. P., Howat D., Rowland T., Walton F. Autoantibody to heat-shock protein 90 can mediate protection against systemic candidosis. Immunology. 1991 Sep;74(1):20–24. [PMC free article] [PubMed] [Google Scholar]
  12. Matthews R., Burnie J. Cloning of a DNA sequence encoding a major fragment of the 47 kilodalton stress protein homologue of Candida albicans. FEMS Microbiol Lett. 1989 Jul 1;51(1):25–30. doi: 10.1016/0378-1097(89)90071-2. [DOI] [PubMed] [Google Scholar]
  13. Matthews R., Burnie J., Smith D., Clark I., Midgley J., Conolly M., Gazzard B. Candida and AIDS: evidence for protective antibody. Lancet. 1988 Jul 30;2(8605):263–266. doi: 10.1016/s0140-6736(88)92547-0. [DOI] [PubMed] [Google Scholar]
  14. Mierendorf R. C., Percy C., Young R. A. Gene isolation by screening lambda gt11 libraries with antibodies. Methods Enzymol. 1987;152:458–469. doi: 10.1016/0076-6879(87)52054-7. [DOI] [PubMed] [Google Scholar]
  15. Strockbine N. A., Largen M. T., Zweibel S. M., Buckley H. R. Identification and molecular weight characterization of antigens from Candida albicans that are recognized by human sera. Infect Immun. 1984 Feb;43(2):715–721. doi: 10.1128/iai.43.2.715-721.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Young D., Lathigra R., Hendrix R., Sweetser D., Young R. A. Stress proteins are immune targets in leprosy and tuberculosis. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4267–4270. doi: 10.1073/pnas.85.12.4267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zhong G., Brunham R. C. Antibody responses to the chlamydial heat shock proteins hsp60 and hsp70 are H-2 linked. Infect Immun. 1992 Aug;60(8):3143–3149. doi: 10.1128/iai.60.8.3143-3149.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Zhong G., Brunham R. C. Antigenic analysis of the chlamydial 75-kilodalton protein. Infect Immun. 1992 Mar;60(3):1221–1224. doi: 10.1128/iai.60.3.1221-1224.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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