Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1996 Nov;149(5):1651–1660.

Expression of the beta 7 integrin by human endothelial cells.

R I Brezinschek 1, H P Brezinschek 1, A I Lazarovits 1, P E Lipsky 1, N Oppenheimer-Marks 1
PMCID: PMC1865263  PMID: 8909254

Abstract

Integrin adhesion receptors mediate fundamental intercellular interactions of many cell types as well as cellular interactions with specific extracellular matrix molecules. To date, the beta 7 integrin has been shown to be expressed by leukocyte subsets and to mediate interactions of these cells with extracellular matrix molecules as well as with endothelial and epithelial cells. The data presented here indicate that human endothelial cells also express the beta 7 integrin both in vitro and in situ. Analysis of cDNA indicated that endothelial beta 7 was identical to that expressed by leukocytes. Cell surface expression of beta 7 was increased by exposure of the endothelium to the pro-inflammatory cytokines, tumor necrosis factor-alpha and interleukin-1 beta. In leukocytes, beta 7 complexes with alpha 4 or alpha E integrin chains. Endothelial cells also expressed a number of alpha-integrin chains, including alpha 4, but not alpha E. The expression and utilization of beta 7, presumably complexed with alpha 4, by endothelial cells may be instrumental in the maintenance of the function or phenotype of endothelial cells.

Full text

PDF
1654

Images in this article

1654Figure 2
on p.1654
1654Figure 4
on p.1654
1656Figure 6
on p.1656
1658Figure 7
on p.1658

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albelda S. M., Buck C. A. Integrins and other cell adhesion molecules. FASEB J. 1990 Aug;4(11):2868–2880. [PubMed] [Google Scholar]
  2. Andrew D. P., Berlin C., Honda S., Yoshino T., Hamann A., Holzmann B., Kilshaw P. J., Butcher E. C. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994 Nov 1;153(9):3847–3861. [PubMed] [Google Scholar]
  3. Bellón T., López-Rodríguez C., Rubio M. A., Jochems G., Bernabeu C., Corbi A. L. Regulated expression of p150,95 (CD11c/CD18; alpha X/beta 2) and VLA-4 (CD49d/CD29; alpha 4/beta 1) integrins during myeloid cell differentiation. Eur J Immunol. 1994 Jan;24(1):41–47. doi: 10.1002/eji.1830240107. [DOI] [PubMed] [Google Scholar]
  4. Brezinschek H. P., Brezinschek R. I., Lipsky P. E. Analysis of the heavy chain repertoire of human peripheral B cells using single-cell polymerase chain reaction. J Immunol. 1995 Jul 1;155(1):190–202. [PubMed] [Google Scholar]
  5. Brezinschek R. I., Lipsky P. E., Galea P., Vita R., Oppenheimer-Marks N. Phenotypic characterization of CD4+ T cells that exhibit a transendothelial migratory capacity. J Immunol. 1995 Apr 1;154(7):3062–3077. [PubMed] [Google Scholar]
  6. Cepek K. L., Shaw S. K., Parker C. M., Russell G. J., Morrow J. S., Rimm D. L., Brenner M. B. Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin. Nature. 1994 Nov 10;372(6502):190–193. doi: 10.1038/372190a0. [DOI] [PubMed] [Google Scholar]
  7. Dejana E., Raiteri M., Resnati M., Lampugnani M. G. Endothelial integrins and their role in maintaining the integrity of the vessel wall. Kidney Int. 1993 Jan;43(1):61–65. doi: 10.1038/ki.1993.11. [DOI] [PubMed] [Google Scholar]
  8. Dustin M. L., Springer T. A. Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells. J Cell Biol. 1988 Jul;107(1):321–331. doi: 10.1083/jcb.107.1.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Erle D. J., Briskin M. J., Butcher E. C., Garcia-Pardo A., Lazarovits A. I., Tidswell M. Expression and function of the MAdCAM-1 receptor, integrin alpha 4 beta 7, on human leukocytes. J Immunol. 1994 Jul 15;153(2):517–528. [PubMed] [Google Scholar]
  10. Favaloro E. J. Differential expression of surface antigens on activated endothelium. Immunol Cell Biol. 1993 Dec;71(Pt 6):571–581. doi: 10.1038/icb.1993.63. [DOI] [PubMed] [Google Scholar]
  11. Hynes R. O. Integrins: a family of cell surface receptors. Cell. 1987 Feb 27;48(4):549–554. doi: 10.1016/0092-8674(87)90233-9. [DOI] [PubMed] [Google Scholar]
  12. Lazarovits A. I., Moscicki R. A., Kurnick J. T., Camerini D., Bhan A. K., Baird L. G., Erikson M., Colvin R. B. Lymphocyte activation antigens. I. A monoclonal antibody, anti-Act I, defines a new late lymphocyte activation antigen. J Immunol. 1984 Oct;133(4):1857–1862. [PubMed] [Google Scholar]
  13. Matsubara T., Ziff M. Basement membrane thickening of postcapillary venules and capillaries in rheumatoid synovium. Immunoelectron microscopic and electron microscopic morphometric analysis. Arthritis Rheum. 1987 Jan;30(1):18–30. doi: 10.1002/art.1780300103. [DOI] [PubMed] [Google Scholar]
  14. Munro J. M., Pober J. S., Cotran R. S. Tumor necrosis factor and interferon-gamma induce distinct patterns of endothelial activation and associated leukocyte accumulation in skin of Papio anubis. Am J Pathol. 1989 Jul;135(1):121–133. [PMC free article] [PubMed] [Google Scholar]
  15. Oppenheimer-Marks N., Davis L. S., Lipsky P. E. Human T lymphocyte adhesion to endothelial cells and transendothelial migration. Alteration of receptor use relates to the activation status of both the T cell and the endothelial cell. J Immunol. 1990 Jul 1;145(1):140–148. [PubMed] [Google Scholar]
  16. Oppenheimer-Marks N., Ziff M. Binding of normal human mononuclear cells to blood vessels in rheumatoid arthritis synovial membrane. Arthritis Rheum. 1986 Jun;29(6):789–792. doi: 10.1002/art.1780290613. [DOI] [PubMed] [Google Scholar]
  17. Osborn L., Hession C., Tizard R., Vassallo C., Luhowskyj S., Chi-Rosso G., Lobb R. Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes. Cell. 1989 Dec 22;59(6):1203–1211. doi: 10.1016/0092-8674(89)90775-7. [DOI] [PubMed] [Google Scholar]
  18. Paraskeva C., Buckle B. G., Thorpe P. E. Selective killing of contaminating human fibroblasts in epithelial cultures derived from colorectal tumours using an anti Thy-1 antibody-ricin conjugate. Br J Cancer. 1985 Jan;51(1):131–134. doi: 10.1038/bjc.1985.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Parker C. M., Cepek K. L., Russell G. J., Shaw S. K., Posnett D. N., Schwarting R., Brenner M. B. A family of beta 7 integrins on human mucosal lymphocytes. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1924–1928. doi: 10.1073/pnas.89.5.1924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pober J. S., Bevilacqua M. P., Mendrick D. L., Lapierre L. A., Fiers W., Gimbrone M. A., Jr Two distinct monokines, interleukin 1 and tumor necrosis factor, each independently induce biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells. J Immunol. 1986 Mar 1;136(5):1680–1687. [PubMed] [Google Scholar]
  21. Pober J. S., Cotran R. S. The role of endothelial cells in inflammation. Transplantation. 1990 Oct;50(4):537–544. doi: 10.1097/00007890-199010000-00001. [DOI] [PubMed] [Google Scholar]
  22. Postigo A. A., Sánchez-Mateos P., Lazarovits A. I., Sánchez-Madrid F., de Landázuri M. O. Alpha 4 beta 7 integrin mediates B cell binding to fibronectin and vascular cell adhesion molecule-1. Expression and function of alpha 4 integrins on human B lymphocytes. J Immunol. 1993 Sep 1;151(5):2471–2483. [PubMed] [Google Scholar]
  23. Schweighoffer T., Tanaka Y., Tidswell M., Erle D. J., Horgan K. J., Luce G. E., Lazarovits A. I., Buck D., Shaw S. Selective expression of integrin alpha 4 beta 7 on a subset of human CD4+ memory T cells with Hallmarks of gut-trophism. J Immunol. 1993 Jul 15;151(2):717–729. [PubMed] [Google Scholar]
  24. Springer T. A. Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425–434. doi: 10.1038/346425a0. [DOI] [PubMed] [Google Scholar]
  25. Takada Y., Elices M. J., Crouse C., Hemler M. E. The primary structure of the alpha 4 subunit of VLA-4: homology to other integrins and a possible cell-cell adhesion function. EMBO J. 1989 May;8(5):1361–1368. doi: 10.1002/j.1460-2075.1989.tb03516.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tiisala S., Hakkarainen M., Majuri M. L., Mattila P. S., Mattila P., Renkonen R. Down-regulation of monocytic VLA-4 leads to a decreased adhesion to VCAM-1. FEBS Lett. 1993 Oct 11;332(1-2):19–23. doi: 10.1016/0014-5793(93)80473-8. [DOI] [PubMed] [Google Scholar]
  27. Tiisala S., Paavonen T., Renkonen R. Alpha E beta 7 and alpha 4 beta 7 integrins associated with intraepithelial and mucosal homing, are expressed on macrophages. Eur J Immunol. 1995 Feb;25(2):411–417. doi: 10.1002/eji.1830250216. [DOI] [PubMed] [Google Scholar]
  28. Yuan Q. A., Jiang W. M., Krissansen G. W., Watson J. D. Cloning and sequence analysis of a novel beta 2-related integrin transcript from T lymphocytes: homology of integrin cysteine-rich repeats to domain III of laminin B chains. Int Immunol. 1990;2(11):1097–1108. doi: 10.1093/intimm/2.11.1097. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES