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. 1994 Jun;62(6):2605–2608. doi: 10.1128/iai.62.6.2605-2608.1994

Protection of gerbils from amebic liver abscess by immunization with a recombinant protein derived from the 170-kilodalton surface adhesin of Entamoeba histolytica.

T Zhang 1, S L Stanley Jr 1
PMCID: PMC186551  PMID: 8188384

Abstract

The protozoan parasite Entamoeba histolytica causes extensive morbidity and mortality worldwide through intestinal infection and amebic liver abscess. Here we show that vaccination of gerbils, a standard model for amebic liver abscess, with recombinant proteins derived from the 170-kDa galactose-binding adhesin of E. histolytica and the serine-rich E. histolytica protein or a combination of the two recombinant antigens provides excellent protection against subsequent hepatic challenge with virulent E. histolytica trophozoites.

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Selected References

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  1. Braga L. L., Ninomiya H., McCoy J. J., Eacker S., Wiedmer T., Pham C., Wood S., Sims P. J., Petri W. A., Jr Inhibition of the complement membrane attack complex by the galactose-specific adhesion of Entamoeba histolytica. J Clin Invest. 1992 Sep;90(3):1131–1137. doi: 10.1172/JCI115931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cieslak P. R., Virgin H. W., 4th, Stanley S. L., Jr A severe combined immunodeficient (SCID) mouse model for infection with Entamoeba histolytica. J Exp Med. 1992 Dec 1;176(6):1605–1609. doi: 10.1084/jem.176.6.1605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ghadirian E., Meerovitch E., Hartmann D. P. Protection against amebic liver abscess in hamsters by means of immunization with amebic antigen and some of its fractions. Am J Trop Med Hyg. 1980 Sep;29(5):779–784. doi: 10.4269/ajtmh.1980.29.779. [DOI] [PubMed] [Google Scholar]
  4. Ghadirian E., Meerovitch E. Vaccination against hepatic amebiasis in hamsters. J Parasitol. 1978 Aug;64(4):742–743. [PubMed] [Google Scholar]
  5. Guan K. L., Dixon J. E. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem. 1991 Feb 1;192(2):262–267. doi: 10.1016/0003-2697(91)90534-z. [DOI] [PubMed] [Google Scholar]
  6. Jiménez Cardoso J. M., Jiménez E., de Jesús Bernal M., Kumate J. Inducción de inmunidad protectora antiamibiana en hamsters con antígenos heterólogos. Rev Invest Clin. 1989 Apr-Jun;41(2):133–138. [PubMed] [Google Scholar]
  7. Myung K., Burch D., Jackson T. F., Stanley S. L., Jr Serodiagnosis of invasive amebiasis using a recombinant Entamoeba histolytica antigen-based ELISA. Arch Med Res. 1992;23(2):285–288. [PubMed] [Google Scholar]
  8. Nain P. C., Vinayak V. K. Elicitation of protective immunity to Entamoeba histolytica--an experimental study. Immunol Cell Biol. 1987 Jun;65(Pt 3):217–222. doi: 10.1038/icb.1987.24. [DOI] [PubMed] [Google Scholar]
  9. Petri W. A., Jr, Ravdin J. I. Protection of gerbils from amebic liver abscess by immunization with the galactose-specific adherence lectin of Entamoeba histolytica. Infect Immun. 1991 Jan;59(1):97–101. doi: 10.1128/iai.59.1.97-101.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Petri W. A., Jr, Smith R. D., Schlesinger P. H., Murphy C. F., Ravdin J. I. Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. J Clin Invest. 1987 Nov;80(5):1238–1244. doi: 10.1172/JCI113198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Purdy J. E., Mann B. J., Shugart E. C., Petri W. A., Jr Analysis of the gene family encoding the Entamoeba histolytica galactose-specific adhesin 170-kDa subunit. Mol Biochem Parasitol. 1993 Nov;62(1):53–59. doi: 10.1016/0166-6851(93)90177-y. [DOI] [PubMed] [Google Scholar]
  12. Ravdin J. I. Entamoeba histolytica: from adherence to enteropathy. J Infect Dis. 1989 Mar;159(3):420–429. doi: 10.1093/infdis/159.3.420. [DOI] [PubMed] [Google Scholar]
  13. Schain D. C., Salata R. A., Ravdin J. I. Human T-lymphocyte proliferation, lymphokine production, and amebicidal activity elicited by the galactose-inhibitable adherence protein of Entamoeba histolytica. Infect Immun. 1992 May;60(5):2143–2146. doi: 10.1128/iai.60.5.2143-2146.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sharma A., Haq A., Ahmad S., Lederer E. Vaccination of rabbits against Entamoeba histolytica with aqueous suspensions of trehalose-dimycolate as the adjuvant. Infect Immun. 1985 Jun;48(3):634–637. doi: 10.1128/iai.48.3.634-637.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zhang T., Cieslak P. R., Stanley S. L., Jr Protection of gerbils from amebic liver abscess by immunization with a recombinant Entamoeba histolytica antigen. Infect Immun. 1994 Apr;62(4):1166–1170. doi: 10.1128/iai.62.4.1166-1170.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zhang Y., Li E., Jackson T. F., Zhang T., Gathiram V., Stanley S. L., Jr Use of a recombinant 170-kilodalton surface antigen of Entamoeba histolytica for serodiagnosis of amebiasis and identification of immunodominant domains of the native molecule. J Clin Microbiol. 1992 Nov;30(11):2788–2792. doi: 10.1128/jcm.30.11.2788-2792.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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