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. 1994 Jun;62(6):2639–2643. doi: 10.1128/iai.62.6.2639-2643.1994

Diversity of the P2 protein among nontypeable Haemophilus influenzae isolates.

J Bell 1, S Grass 1, D Jeanteur 1, R S Munson Jr 1
PMCID: PMC186559  PMID: 8188390

Abstract

The genes for outer membrane protein P2 of four nontypeable Haemophilus influenzae strains were cloned and sequenced. The derived amino acid sequences were compared with the outer membrane protein P2 sequence from H. influenzae type b MinnA and the sequences of P2 from three additional nontypeable H. influenzae strains. The sequences were 76 to 94% identical. The sequences had regions with considerable variability separated by regions which were highly conserved. The variable regions mapped to putative surface-exposed loops of the protein.

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Selected References

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  1. Barenkamp S. J., Leininger E. Cloning, expression, and DNA sequence analysis of genes encoding nontypeable Haemophilus influenzae high-molecular-weight surface-exposed proteins related to filamentous hemagglutinin of Bordetella pertussis. Infect Immun. 1992 Apr;60(4):1302–1313. doi: 10.1128/iai.60.4.1302-1313.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barenkamp S. J., Munson R. S., Jr, Granoff D. M. Outer membrane protein and biotype analysis of pathogenic nontypable Haemophilus influenzae. Infect Immun. 1982 May;36(2):535–540. doi: 10.1128/iai.36.2.535-540.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barnes W. M. Sequencing DNA with dideoxyribonucleotides as chain terminators: hints and strategies for big projects. Methods Enzymol. 1987;152:538–556. doi: 10.1016/0076-6879(87)52060-2. [DOI] [PubMed] [Google Scholar]
  4. Chong P., Yang Y. P., Fahim R., McVerry P., Sia C., Klein M. Immunogenicity of overlapping synthetic peptides covering the entire sequence of Haemophilus influenzae type b outer membrane protein P2. Infect Immun. 1993 Jun;61(6):2653–2661. doi: 10.1128/iai.61.6.2653-2661.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cowan S. W., Schirmer T., Rummel G., Steiert M., Ghosh R., Pauptit R. A., Jansonius J. N., Rosenbusch J. P. Crystal structures explain functional properties of two E. coli porins. Nature. 1992 Aug 27;358(6389):727–733. doi: 10.1038/358727a0. [DOI] [PubMed] [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Forbes K. J., Bruce K. D., Ball A., Pennington T. H. Variation in length and sequence of porin (ompP2) alleles of non-capsulate Haemophilus influenzae. Mol Microbiol. 1992 Aug;6(15):2107–2112. doi: 10.1111/j.1365-2958.1992.tb01384.x. [DOI] [PubMed] [Google Scholar]
  8. Giebink G. S. The microbiology of otitis media. Pediatr Infect Dis J. 1989 Jan;8(1 Suppl):S18–S20. [PubMed] [Google Scholar]
  9. Groeneveld K., van Alphen L., Voorter C., Eijk P. P., Jansen H. M., Zanen H. C. Antigenic drift of Haemophilus influenzae in patients with chronic obstructive pulmonary disease. Infect Immun. 1989 Oct;57(10):3038–3044. doi: 10.1128/iai.57.10.3038-3044.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haase E. M., Campagnari A. A., Sarwar J., Shero M., Wirth M., Cumming C. U., Murphy T. F. Strain-specific and immunodominant surface epitopes of the P2 porin protein of nontypeable Haemophilus influenzae. Infect Immun. 1991 Apr;59(4):1278–1284. doi: 10.1128/iai.59.4.1278-1284.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jeanteur D., Lakey J. H., Pattus F. The bacterial porin superfamily: sequence alignment and structure prediction. Mol Microbiol. 1991 Sep;5(9):2153–2164. doi: 10.1111/j.1365-2958.1991.tb02145.x. [DOI] [PubMed] [Google Scholar]
  12. Joshi A. K., Baichwal V., Ames G. F. Rapid polymerase chain reaction amplification using intact bacterial cells. Biotechniques. 1991 Jan;10(1):42, 44-5. [PubMed] [Google Scholar]
  13. Martin D., Munson R., Jr, Grass S., Chong P., Hamel J., Zobrist G., Klein M., Brodeur B. R. Mapping of B-cell epitopes on the outer membrane P2 porin protein of Haemophilus influenzae by using recombinant proteins and synthetic peptides. Infect Immun. 1991 Apr;59(4):1457–1464. doi: 10.1128/iai.59.4.1457-1464.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Munson R. S., Jr, Kabeer M. H., Lenoir A. A., Granoff D. M. Epidemiology and prospects for prevention of disease due to Haemophilus influenzae in developing countries. Rev Infect Dis. 1989 May-Jun;11 (Suppl 3):S588–S597. doi: 10.1093/clinids/11.supplement_3.s588. [DOI] [PubMed] [Google Scholar]
  15. Munson R. S., Jr, Shenep J. L., Barenkamp S. J., Granoff D. M. Purification and comparison of outer membrane protein P2 from Haemophilus influenzae type b isolates. J Clin Invest. 1983 Aug;72(2):677–684. doi: 10.1172/JCI111017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Munson R., Jr, Bailey C., Grass S. Diversity of the outer membrane protein P2 gene from major clones of Haemophilus influenzae type b. Mol Microbiol. 1989 Dec;3(12):1797–1803. doi: 10.1111/j.1365-2958.1989.tb00165.x. [DOI] [PubMed] [Google Scholar]
  17. Munson R., Jr, Tolan R. W., Jr Molecular cloning, expression, and primary sequence of outer membrane protein P2 of Haemophilus influenzae type b. Infect Immun. 1989 Jan;57(1):88–94. doi: 10.1128/iai.57.1.88-94.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Murphy T. F., Apicella M. A. Nontypable Haemophilus influenzae: a review of clinical aspects, surface antigens, and the human immune response to infection. Rev Infect Dis. 1987 Jan-Feb;9(1):1–15. doi: 10.1093/clinids/9.1.1. [DOI] [PubMed] [Google Scholar]
  19. Murphy T. F., Bartos L. C. Human bactericidal antibody response to outer membrane protein P2 of nontypeable Haemophilus influenzae. Infect Immun. 1988 Oct;56(10):2673–2679. doi: 10.1128/iai.56.10.2673-2679.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Murphy T. F., Dudas K. C., Mylotte J. M., Apicella M. A. A subtyping system for nontypable Haemophilus influenzae based on outer-membrane proteins. J Infect Dis. 1983 May;147(5):838–846. doi: 10.1093/infdis/147.5.838. [DOI] [PubMed] [Google Scholar]
  21. Musser J. M., Barenkamp S. J., Granoff D. M., Selander R. K. Genetic relationships of serologically nontypable and serotype b strains of Haemophilus influenzae. Infect Immun. 1986 Apr;52(1):183–191. doi: 10.1128/iai.52.1.183-191.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pauptit R. A., Schirmer T., Jansonius J. N., Rosenbusch J. P., Parker M. W., Tucker A. D., Tsernoglou D., Weiss M. S., Schultz G. E. A common channel-forming motif in evolutionarily distant porins. J Struct Biol. 1991 Oct;107(2):136–145. doi: 10.1016/1047-8477(91)90017-q. [DOI] [PubMed] [Google Scholar]
  23. Sikkema D. J., Murphy T. F. Molecular analysis of the P2 porin protein of nontypeable Haemophilus influenzae. Infect Immun. 1992 Dec;60(12):5204–5211. doi: 10.1128/iai.60.12.5204-5211.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Srikumar R., Dahan D., Gras M. F., Ratcliffe M. J., van Alphen L., Coulton J. W. Antigenic sites on porin of Haemophilus influenzae type b: mapping with synthetic peptides and evaluation of structure predictions. J Bacteriol. 1992 Jun;174(12):4007–4016. doi: 10.1128/jb.174.12.4007-4016.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Srikumar R., Dahan D., Gras M. F., Saarinen L., Käyhty H., Sarvas M., Vogel L., Coulton J. W. Immunological properties of recombinant porin of Haemophilus influenzae type b expressed in Bacillus subtilis. Infect Immun. 1993 Aug;61(8):3334–3341. doi: 10.1128/iai.61.8.3334-3341.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Struyvé M., Visser J., Adriaanse H., Benz R., Tommassen J. Topology of PhoE porin: the 'eyelet' region. Mol Microbiol. 1993 Jan;7(1):131–140. doi: 10.1111/j.1365-2958.1993.tb01104.x. [DOI] [PubMed] [Google Scholar]
  27. Vachon V., Lyew D. J., Coulton J. W. Transmembrane permeability channels across the outer membrane of Haemophilus influenzae type b. J Bacteriol. 1985 Jun;162(3):918–924. doi: 10.1128/jb.162.3.918-924.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wald E. R. Haemophilus influenzae as a cause of acute otitis media. Pediatr Infect Dis J. 1989 Jan;8(1 Suppl):S28–S30. [PubMed] [Google Scholar]
  29. Weinberg G. A., Ghafoor A., Ishaq Z., Nomani N. K., Kabeer M., Anwar F., Burney M. I., Qureshi A. W., Musser J. M., Selander R. K. Clonal analysis of Hemophilus influenzae isolated from children from Pakistan with lower respiratory tract infections. J Infect Dis. 1989 Oct;160(4):634–643. doi: 10.1093/infdis/160.4.634. [DOI] [PubMed] [Google Scholar]
  30. Weiss M. S., Kreusch A., Schiltz E., Nestel U., Welte W., Weckesser J., Schulz G. E. The structure of porin from Rhodobacter capsulatus at 1.8 A resolution. FEBS Lett. 1991 Mar 25;280(2):379–382. doi: 10.1016/0014-5793(91)80336-2. [DOI] [PubMed] [Google Scholar]
  31. van Alphen L., Eijk P., Geelen-van den Broek L., Dankert J. Immunochemical characterization of variable epitopes of outer membrane protein P2 of nontypeable Haemophilus influenzae. Infect Immun. 1991 Jan;59(1):247–252. doi: 10.1128/iai.59.1.247-252.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. van Alphen L. Epidemiology and prevention of respiratory tract infections due to nonencapsulated Haemophilus influenzae. J Infect Dis. 1992 Jun;165 (Suppl 1):S177–S180. doi: 10.1093/infdis/165-supplement_1-s177. [DOI] [PubMed] [Google Scholar]

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