Abstract
Purothionins are basic polypeptides with antimicrobial properties that are present in the endosperm of wheat and other Gramineae. Susceptibility to crude and electrophoretically purified purothionins among brewing starters has been investigated. Seven yeast strains of Saccharomyces uvarum (syn. carlsbergensis), four strains of Saccharomyces cerevisiae, and four wild strains (Saccharomyces spp.) have been tested in three culture media. All the strains were susceptible to the crude preparation in a yeast extract-glucose medium. Determinations of minimal inhibitory and biocidal concentrations yielded double end points in about half of the assays. The highest sensitivity to purothionins was obtained in malt extract medium. Sensitivity to electrophoretically purified purothionins was of the same order or smaller than to the crude preparation. Possible explanations for this unexpected result are presented.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Carbonero P., García-Olmedo F. Purothionins in Aegilops-Triticum spp. Experientia. 1969 Oct 15;25(10):1110–1111. doi: 10.1007/BF01901464. [DOI] [PubMed] [Google Scholar]
- ERLNAGER B. F., GOODE L. Gramicidin S; relationship of cyclic structure to antibiotic activity. Nature. 1954 Oct 30;174(4435):840–841. doi: 10.1038/174840a0. [DOI] [PubMed] [Google Scholar]
- Fernandez de Caleya R., Gonzalez-Pascual B., García-Olmedo F., Carbonero P. Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro. Appl Microbiol. 1972 May;23(5):998–1000. doi: 10.1128/am.23.5.998-1000.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Popescu M., Lazarus L. H., Goldblum N. Simplified adaptor for electroelution. Anal Biochem. 1971 Apr;40(2):247–253. doi: 10.1016/0003-2697(71)90383-6. [DOI] [PubMed] [Google Scholar]